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Creep response of a generalized Maxwell model

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An electrolysis process method for free-form blade surface finishing is proposed for a free-form surface impeller, and a stepwise method is used to process the inter-blade channel of the overall impeller. The forming cathode is then used to finish the blade to meet the blade processing requirements. In the design, the forming cathode structure was improved by using motion simulation software, and the flow field simulation software was used to simulate and analyze the cathode flow channel. The cathode shape and the electrolyte flow rate between the electrodes meet the processing requirements. In the process of processing experiments, the motion path of the cathode was analyzed and optimized. The effect of the feed direction on the uneven distribution of the blade machining gap was reduced through optimization, and high-frequency pulse power processing was used to reduce the machining gap and improve the machining accuracy of the blade. The experimental results show that the process scheme is feasible and the precision of the processed impeller free-form surface is significantly improved. The material is a monolithic turbine disk of high-temperature alloys, and its large twisted blade processing has always been a problem in the manufacturing industry
In this paper, a new exponential formed vertical distribution of nearshore flow velocity is constructed, which is simpler in form and more suitable for engineering application. The physical meaning of the new formula is more specific than that of Soulsby. Compared with those logarithmic formed ones, the new one does not need the maximum velocity and only needs the mean velocity in vertical, which gives it better engineering practicability. Apply the new formula to Jiangsu coastal area and compare the results with that of Soulsby whose results show the new formula agrees better with the measured flow velocity, which reasonably reflects the basic principles of vertical distribution of flow velocity
Endoxifen, an active metabolite of tamoxifen, has been shown to be an effective anti-estrogenic agent in estrogen receptor-positive breast cancer patients. In melanoma, estrogen receptor expression is shown to be associated with disease progression. However, the therapeutic benefit of endoxifen in melanoma has not yet been evaluated. Here, we present the first demonstration of the anti-melanogenic activity of endoxifen in vitro and in vivo. The in vitro cytotoxic effect of endoxifen was tested using a cell viability assay. The in vivo anti-melanogenic activity was evaluated in B16F10 cell-bearing C57BL/6 mice, a mouse melanoma model. The general toxicity was tested in Swiss albino mice. Endoxifen exhibited greater activity against melanoma cell lines. Treatment of B16F10 mouse and SK-MEL-5 human melanoma cell lines with 10 μM of endoxifen for 48 h respectively resulted in 93.6 and 92.5% cell death. Orally administered endoxifen, at dose levels of 4 and 8 mg/kg body weight/day for 20 consecutive days, respectively reduced metastatic melanoma nodules in the lungs by 26.7 and 82.7%. Endoxifen was found to be a safe and effective anti-melanogenic agent in animal studies.
There is large area of saline abandoned and lowyielding land distributed in coastal zone in the world. Soil salinity which inhibits plant growth and decreases crop yield is a serious and chronic problem for agricultural production. Improving plant salt tolerance is a feasible way to solve this problem. Plant physiological and biochemical responses under salinity stress become a hot issue at present, because it can provide insights into how plants may be modified to become more tolerant. It is generally known that the negative effects of soil salinity on plants are ascribed to ion toxicity, oxidative stress and osmotic stress, and great progress has been made in the study on molecular and physiological mechanisms of plant salinity tolerance in recent years. However, the present knowledge is not easily applied in the agronomy research under field environment. In this review, we simplified the physiological adaptive mechanisms in plants grown in saline soil and put forward a practical procedure for discerning physiological status and responses. In our opinion, this procedure consists of two steps. First, negative effects of salt stress are evaluated by the changes in biomass, crop yield and photosynthesis. Second, the underlying reasons are analyzed from osmotic regulation, antioxidant response and ion homeostasis. Photosynthesis is a good indicator of the harmful effects of saline soil on plants because of its close relation with crop yield and high sensitivity to environmental stress. Particularly, chlorophyll a fluorescence transient has been accepted as a reliable, sensitive and convenient tool in photosynthesis research in recent years, and it can facilitate and enrich photosynthetic research under field environment.
Glutamine synthetase (GS) plays fundamental roles in nitrogen metabolism in higher plants. Ramie (Boehmeria nivea L. Gaud) has excellent forage and rapid growth characteristics, and isolated BnGS1-2 is closely related to that of legumes, potentially providing gene resources for nitrogen uptake and assimilation. Consequentially, transgenic studies involving the over-expression of BnGS1-2 in Nicotiana tabacum were performed to investigate the potential function and application of BnGS1-2 in higher plants. Comparative analysis of nitrogen use efficiency (NUE) was performed during wild type and independent transgenic lines with various in GS activity at the mRNA and protein levels. Our results indicate that the BnGS1-2-over-expressing transgenic lines significantly enhanced the fresh weight, dry weight and plant height. Simultaneously, the transgenic lines had higher soluble protein content and higher total nitrogen content than the control plants under normal planting condition. The range of enhancement positively associated with BnGS1-2 activities in different transgenic lines. The over-expression of BnGS1-2 in tobacco resulted in a significant decrease in leaf-free NH4+ content. Simultaneously, the transgenic lines increased the NO3- uptake and NH4+ assimilation with a remarkable increase in the NR activity and no change in the free NO3- content. These results indicate that BnGS1-2would be an excellent gene resource for improvement of plants biomass and NUE.
Gas exchange, chlorophyll a fluorescence and modulated 820 nm reflection were investigated to explore the development of photosynthesis in Jerusalem artichoke (Helianthus tuberosus L.) leaves from initiation to full expansion. During leaf expansion, photosynthetic rate (Pn) increased and reached the maximal level when leaves were fully expanded. The same change pattern was also found in the stomatal conductance and chlorophyll content. Lower Pn could not be ascribed to the higher stomatal resistance in developing leaves, as intercellular CO₂ concentration was not significantly lower in these leaves. Lower Pn partly resulted from the lower actual photochemical efficiency of PSII in developing leaves, as more excited energy was dissipated through non-photochemical quenching. The development of primary photochemical reaction and electron transport in the donor side of PSII was completed in the initiating leaves. However, the development of electron transport in the acceptor side of PSII was not accomplished until leaves were fully expanded, indicated by the change in probability that an electron moves further than primary quinone (ψo). PSI activity changed in parallel with ψo suggesting that PSI cooperated well with PSII during leaf expansion. It should be stressed that the development of carbon fixation process was later than primary photochemical reaction but earlier than photosynthetic electron transport during leaf expansion. The later development of photosynthetic electron transport may reduce the production of reactive oxygen species from Mehler reaction, particularly under low carbon fixation.
Phytoplasmas are plant pathogenic bacteria that inhabit the phloem sieve elements in infected plants and lack a cell wall. Infection of the woody perennial Ziziphus jujuba with phytoplasma causes jujube witches’ broom (JWB), a disease that causes heavy crop losses. The TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors (TFs) are plant-specific regulators of plant morphogenesis, development, and defense after phytoplasma infection. While the TCP gene family has been studied in many plants, there is no report on TCPs in jujube. In this study, 21 ZjTCP TFs were identified and bioinformatically analyzed based on the genome of the Dongzao cultivar. Another 2 ZjTCPs were found in the genome of the Junzao cultivar. Physicochemical properties of the ZjTCP proteins were quite varied, indicating possible versatility of functions. ZjTCP proteins ranged from 172 to 590 amino acids (aa), had isoelectric points (pIs) between 5.53 (ZjTCP1) and 9.81 (ZjTCP11), and molecular weights ranging from 19,279.98 (ZjTCP11) to 61,784.17 kDa (ZjTCP19). Interaction network mapping predicted several hubs, such as ZjTCP6, ZjTCP7, ZjTCP8, ZjTCP15, ZjTCP16, and ZjTCP19, among which ZjTCP6 and ZjTCP16 were predicted to function in plant defense and morphogenesis. Spatiotemporal expression analysis of the ZjTCPs showed that ten of the ZjTCP genes were detected after infection with ‘Candidatus Phytoplasma ziziphi’. ZjTCP6, ZjTCP7, ZjTCP16, and ZjTCP19 were up-regulated after phytoplasma infection. ZjTCP16 showed the most significant increase in transcript levels, after the emergence of disease symptoms. ZjTCP12, ZjTCP15, and ZjTCP18 were down-regulated after phytoplasma infection. We concluded that ZjTCP6 and ZjTCP16 were most likely regulatory factors with roles during the plant response to jujube witches’ broom.
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