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1

Effects of repeated lipopolysaccharide treatment on growth performance, immune organ index, and blood parameters of Sprague-Dawley rats

100%
Zhong Y., Zhang X., Hu X., Li Y.
Journal of Veterinary Research
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2018
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tom 62
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nr 3
2

Cloning, expression and identification by immunohistochemistry of humanized single-chain variable fragment antibody against hepatitis C virus core protein

76%
Lun Y., Cheng J., Zhong Y., Yu Z., Wang Q., Wang F., Feng J.
Polish Journal of Microbiology
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2011
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tom 60
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nr 1
Expression of single-chain variable fragment (scFv) antibodies on the surface of bacteriophage is widely used to prepare antibodies with pre-defined specificities. A phage antibody library containing the gene for scFv antibody against Hepatitis C virus core protein was panned with core protein immobilized on microtiter plate wells. After five rounds of panning 60 phage clones specific to core protein were obtained and one selected clone was sequenced. It was found that the specifically detected antigen consists of 774bp and is capable of encoding 257 amino acids in the patients but not in healthy persons.
3

Influence of Br24 and Gr24 on the accumulation and uptake of Cd and As by rice seedlings grown in nutrient solution

76%
Xu B., Yu J., Zhong Y., Guo Y., Ding J., Chen Y., Wang G.
Polish Journal of Environmental Studies
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2019
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tom 28
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nr 5
Plant hormones are trace organic molecules that play important roles in plant growth, development and stress resistance. 24-epibrassinolide (Br24) and strigolactones (Gr24) were reported to alleviate the toxicity of heavy metals and restrict their translocation into plants. In this study, the effects of Br24 and Gr24 with or without iron plaque (IP) induction on the uptake and accumulation of cadmium (Cd) and arsenic (As) were examined in rice seedlings exposed to 6-day treatments of 2 mg L⁻¹ Cd or 5 mg L⁻¹ As in nutrient solution. The results showed that the growth of rice seedlings was decreased by Cd or As treatment, but Br24 or Gr24 with or without IP alleviated this adverse effect. Br24 or Gr24 can improve root length, average diameter, surface area, and volume, and tip the number of roots of rice plants with or without IP. Cadmium contents in rice shoots were significantly reduced by Br24 with IP or Gr24, but Cd accumulation and translocation from root to shoot were not decreased. The interaction of Br24 or Gr24 and IP dramatically increased As contents in rice root, and reduced As translocation from root to shoot. Br24 or Gr24 with IP induction significantly increased Fe content, but decreased the contents of mineral elements (Mn, Cu and Zn) in rice plants. Our results suggested that the exogenous application of Br24 or Gr24 could be advantageous against the toxicity of Cd or As, and a potential way to effectively inhibit Cd or As uptake by rice plants.
4
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Potential risk of Mesodinium rubrum bloom in the aquaculture area of Dapeng’ao cove, China: diurnal changes in the ciliate community structure in the surface water

76%
Liu H., Song X., Huang L., Tan Y., Zhong Y., Huang J. R.
Oceanologia
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2012
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tom 54
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nr 1
Diurnal changes in the structure of the ciliate community in surface waters were studied in the aquaculture area of Dapeng’ao cove, China. Two periods of heavy rainfall occurred during the study period, intensifying water column stratification and influencing the water’s properties. A total of 21 ciliate taxa from 15 genera were identified; the dominant species was Mesodinium rubrum. The maximum abundance of M. rubrum reached 3.92×104 indiv. dm−3, contributing 95.1% (mean value) to the total ciliate abundance. Diurnal changes in M. rubrum abundance were highly variable, the driving force probably being irradiance and food availability. The results suggest that M. rubrum may form blooms in aquaculture areas when there is a suitable physical regime with enriched nutrients, which is potentially harmful to the fish-farming industry.
5

Combined action of an antioxidant defence system and osmolytes on drought tolerance and post-drought recovery of Phoebe zhennan S. Lee saplings

64%
Hu Y., Wang B., Hu T., Chen H., Li H., Zhang W., Zhong Y., Hu H.
Acta Physiologiae Plantarum
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2015
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tom 37
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nr 04
Nanmu (Phoebe zhennan S. Lee) is a wellknown rare tree species in China that is valued as an ornamental garden plant and for its high timber quality. Recently, the cultivation of nanmu has gained attention for use in tree resource conservation and ecological restoration projects. Drought is a major environmental factor that affects the growth and development of plants. In this study, the drought tolerance and post-drought recovery of nanmu, which is associated with antioxidative enzymes and osmotic adjustment, were examined by exposing nanmu saplings to drought for 30 days followed by 10 days of rewatering in a greenhouse. Drought stress resulted in increased levels of osmolytes, sugars and proteins in nanmu leaves compared with the well-watered controls as well as higher concentrations of superoxide radicals and hydrogen peroxide, leading to lipid peroxidation and significantly higher activities of superoxide dismutase, catalase and guaiacol peroxidase and higher levels of ascorbic acid. After re-watering for 5 days, most of the antioxidant enzymes and ascorbic acid were restored to their original levels, whereas the activity of guaiacol peroxidase and the levels of soluble sugar and soluble protein remained markedly high. Moreover, nanmu saplings maintained normal turgor pressure under mild and moderate drought conditions, indicating the presence of a mechanism that affects osmotic adjustment and growth restriction, thus enabling the plant to adjust to drought stress. These results suggested that an antioxidant defence system and osmolytes play important roles in nanmu during drought stress and recovery.
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