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Between 1991 and 1995 fresh fecal samples from Iberian lynx (Lynx pardinus), wildcats (Felis silvestris), red foxes (Vulpes vulpes), and other carnivore species were collected in two areas of central Spain for isolation of parasite eggs and larvae. Twenty-three gastrointestinal coccidia, cestoda and nematoda species were identified. Common (≥20% prevalence) species were Isospora felis, I. rivolta, Ancylostoma spp., Toxascaris leonina, Toxocara cati, Aelurostrongylus spp., and Physaloptera spp. for the wildcat, I. felis, Taenia spp., Ancylostoma spp., T. leonina, and Toxocara canis for the Iberian lynx, and I. canis, I. vulpis, and Physaloptera spp. for the red fox. In contrast to the pattern found in most similar studies, the distribution of parasitic forms among individual hosts was not overdispersed. Differences in prevalence between host populations were only detected for Physaloptera spp. in the wildcat. Sexual differences in occurrence, prevalence and intensity were not found in any host. The number of parasite species per individual was significantly higher in adult than in subadult hosts, and negatively correlated with a rough index of host body condition. The consistence of parasite species across samples of the same individual host taken at different times was low. In this paper we provide the first data on intestinal parasites for the rare Iberian lynx.
Perumytilus purpuratus is an abundant bivalve located in the intertidal rocky zone of South America that has been considered as a key species of the ecosystem. There are few studies of the host-parasite relationship of this bivalve; thus, this research aims to analyse the spatial and temporal variation in the prevalence of trematodes in P. purpuratus. Bivalves were collected from three localities (El Tabo, Las Cruces and Montemar) of central Chile (33°S, 71°W) during different seasons of 2010. The bivalves were also collected every metre, from the lowest to the highest level of the intertidal rocky zone, to determine the parasite distribution within the localities. Three species of trematodes as sporocyst stages were found: Prosorhynchoides carvajali, Proctoeces sp. and an undetermined fellodistomid species. Of the 37,692 bivalve specimens collected, 2.68% were parasitised. The undetermined fellodistomid species was the most prevalent parasite observed (1.69%). There were little detected differences in the prevalence of some trematode species between seasons. The prevalence of P. carvajali varied between localities, being most prevalent at Montemar. The distribution of trematodes along the rocky zone within the localities was variable, with P. carvajali being more prevalent in the mid-lowest level of the intertidal zone and the undetermined fellodistomid species being more prevalent in the mid-highest level. Both the abundance of definitive hosts and the environmental conditions likely result in different levels of infection by trematodes in P. purpuratus between and within the localities.
Activity, spatial ecology, and pattern of sett use of a mediterranean population of badgers Meles meles Linnaeus, 1758 are described. Data come from Doñana National Park (SW Spain) where a 1-year radio-tracking study and a 9-year capture-recapture study were carried out. Badger home ranges were spatially structured, territories having an average size that is the largest reported in the literature. There was a correlation between territory size and ecological variables reflecting food patch dispersion. It is suggested that badgers might set territory limits to include enough patches of rabbit burrows in the critical summer period of low rabbit abundance. Badgers were not strictly nocturnal, and showed decreased activity levels in winter. Activity levels were higher in badgers living in dry habitats, and in all badgers during the dry season. Both photoperiod and rabbit availability were suggested as factors influencing activity parameters like activity length or resting intervals during activity periods. Differences in the pattern of use of the main and secondary setts were found between reproductive females and other individuals.
Hypothyroidism affects neuron population dynamics in the hippocampus of the adult rat, with neuronal damage as the main feature of its effect. This effect is prevented by the blockade of NMDA receptors, which suggests that glutamatergic activity mediates cell death in this condition. Glutamate can also stimulate cell proliferation and survival of newborn neurons, indicating that it can affect different stages of the cell cycle. In this work we measured the expression of specific proteins that control cell proliferation (cycline-Dl), cell arrest (p21), damage (p53) or apoptosis (Bax and Bcl2) in the hippocampus of hypothyroid rats treated with the NMDA receptor (NMDAR) blocker MK-801 during the induction of hypothyroidism. The results show that hypothyroidism increases the expression of markers of DNA damage, cell arrest, and apoptosis, but does not affect the marker of cell proliferation. NMDAR blockade prevents the increase on markers of DNA damage and apoptosis, but does not influence cell arrest or cell proliferation. This suggests that hypothyroidism promotes cell death mainly by an excitotoxic effect of glutamate.
The aim of the present work was to explore physiological changes provoked by somaclonal variation in response to salinity. Two parental cultivars (La Candelaria and Yerua) and their derived somaclones were used as a source for breeding new rice lines with improved salt tolerance. We studied the effect of NaCl salt stress on chlorophyll fluorescence-related parameters, such as the maximum quantum yield of primary PSII photochemistry (Fv/Fm) and the performance index for energy conservation from photon absorbed by PSII antenna (PIABS). In addition malondialdehyde (MDA) content and leaf temperature (LT) responses were also measured. In somaclonal lines, Fv/Fm, PIABS, MDA and LT showed coefficients of variation of 13.7, 39.3, 25.5, and 3 %, respectively, for La Candelaria and 1.4, 17.6, 34.4 and 3 % for Yerua. However, the fragrant character did not differ in the aromatic somaclonal lines with respect to their parentals. Our results suggest that the Fv/Fm ratio would not be as good marker of PSII vitality as PIABS for salinized rice somaclones, unless they are highly susceptible to salinity. On other hand, the MDA content showed a strong negative correlation with the PIABS content in somaclones of both rice cultivars, suggesting that MDA levels could also be used as an oxidative damage index in rice somaclones.
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