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SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues

100%

Babol-Pokora K., Berent J.

Acta Biochimica Polonica

2008

tom 55

nr 4

815-819

SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism®377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.

Ethanol-, methanol, and ethylene glycol-induced oxidative stress in selected rat organs

61%

Jurczyk A., Jankowska B., Barzdo M., Meissner E., Antoszczyk L., Berent J., Szram S.

Acta Biochimica Polonica

2003

tom 50

nr Supplement 1

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Berent J.

1 Antoszczyk L.

1 Babol-Pokora K.

1 Barzdo M.

1 Jankowska B.

1 Jurczyk A.

1 Meissner E.

1 Szram S.

1 2008

1 2003

Wyniki wyszukiwania

SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues

Ethanol-, methanol, and ethylene glycol-induced oxidative stress in selected rat organs