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Sand and soil were experimentally contaminated with eggs of Ascaris suum and oncosphercs of Hymenolepis diminuta in three doses: 500, 3000 and 5000 eggs (oncospheres) per 50 g of the ground. Numbers of recovered eggs were compared with the use of the two following methods: the method of flotation and Spindler's method, modified by Vasilkova and Ivančuk. The ground samples were investigated the following day and on the 14th day after the contamination. The samples were stored in 20°C and in 4°C. The Spindler's modified method turned out to be better for the studies of outdoor samples, because it enabled us to detect eggs in slightly infected samples, and in different temperatures.
At the first experiment 20 Swiss male mice were infected with 100 larvae and 20 mice with 500 larvae T. spiralis per mouse. Two days after infection (d.a.i.) mice orally received 1,5 mg Cd (water solution CdCl₂) each. 40 mice were infected only T. spiralis as conrol. At the second experiment muscle larvae used were isolated from mice (from the first experiment) which received Cd. 20 mice were infected with 100 larvae and 20 - with 500 larvae per mouse. Two d.a.i. mice received 1,5 mg Cd. Mice fom both experiments were killed at 5, 10, 20 and 42 d.a.i. Total number of adult T. spiralis worms present in the small intestine and muscle larvae were recovered by conventional technique. Results of the first experiment: the number of adult worms and muscle larvae recovered from mice received Cd. were statistically significant lower as in control. Results of the second experiment the mean number of adult worms in experimental group and in control were the same but the mean number of larvae per gram of mice muscle were significant higher as in control.
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