Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 16

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Badanie dzialania cytotoksycznego substancji chemicznych

100%
Skierski J.
Postępy Biologii Komórki. Suplement
|
2008
|
nr 24
147-163
2

Racjonalizacja zuzycia energii przez uklady napedowe potrzeb wlasnych elektrowni

63%
Skierski J., Buchta J.
Gospodarka Paliwami i Energią
|
2003
|
tom 51
|
nr 05-06
23-26
3

Effects of inhibitor of Src kinases, SU6656, on differentiation of megakaryocytic progenitors and activity of alpha1,6-fucosyltransferase

51%
Kaminska J., Klimczak-Jajor E., Skierski J., Bany-Laszewicz U.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 3
499-506
α1,6-fucosyltransferase (FUT8) attaches fucose residues viaan α1,6 linkage to the innermost N-acetylglucosamine residue of N-linked glycans. Glycans with this type of structure are present in GpIIb/GpIIIa complex (CD41a) which is present on megakaryocytes (Mks) and platelets. CD41a is the earliest marker of megakaryocytopoiesis. The aim of this study was to analyse the morphology, phenotype, ploidy level and activity of FUT8 during induced differentiation/maturation of Mk progenitor cells in ex vivoculture. We used SU6656, a selective inhibitor of Src tyrosine kinases, as differentiation-inducing agent for Mks. The addition of SU6656 to the culture system of megakaryocytic progenitors from cord blood CD34+cells and Meg-01 cell line induced their maturation towards later stages of Mk differentiation with increased activity of FUT8. We suggest FUT8 as a candidate for an early marker of differentiation and possibly of the ploidy level of Mks. We confirm a special status of FUT8 in megakaryocytopoiesis.
4

Proliferation and apoptosis of human T cells during replicative senescence - a critical approach

51%
Jaruga E., Skierski J., Radziszewska E., Sikora E.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 2
Normal human T lymphocytes growing in culture undergo replicative senescence. Previously, we have shown that in our conditions polyclonal T cells cease proliferation after about three weeks (Radziszewska et al., 1999, Cell Biol. Int. 23, 97-103). Now we present results of a more detailed analysis of in vitro growth as well as phenotypic changes of T cells. Cell cycle analysis showed that about 20% of cells were in the S phase untill the 17th day of culture (young cells). The highest number of mitotic cells (phase G2/M; 10%) was observed during the first week of culture. All not dividing senescent cells were stopped in the G1 phase (after the 30th day of culture). The sub-G1 fraction which represents apoptotic cells did not exceed 8% during the whole period until the 30th day of culture. During in vitro T-cell growth, a rather rapid selection to CD3+CD8+ cells occurs. In the presenescent (between the 17th and 30th day) and senescent populations the majority of cells (above 90%) were CD8 positive. We also have checked the expression of alpha-chain interleukin-2 (IL-2) receptor (CD25). In young and presenescent cells about one third of cells was CD25 positive, but only 15% in the pool of senescent cells. Immunoblotting analysis of p16 protein recognized previously as a marker of senescent T cells, showed its highest and transient expression in presenescent cells. A critical review of the polyclonal T cell replicative senescence model is presented.
5

Synergistic interactions of nitroxides and doxorubicin in the induction of apoptosis: the proapoptotic effect of pirolin and tempace in immortalized rodent fibroblasts

51%
Koceva-Chyla A., Gwozdzinski K., Skierski J., Jozwiak Z.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
6

Somatic embryogenesis of Gentiana genus. III. Characterization of three-year-old embryogenic suspensions of G.pannonica originated from various seedling explants

51%
Mikula A., Skierski J., Rybczynski J. J.
Acta Physiologiae Plantarum
|
2002
|
tom 24
|
nr 3
7

Stability of 2-chloro-2'-deoxyadenosine at various pH and temperature

51%
Tarasiuk A., Skierski J., Kazimierczuk Z.
Archivum Immunologiae et Therapiae Experimentalis
|
1994
|
tom 42
|
nr 1
8

Effect of aging on UVC-induced apoptosis of rat splenocytes

45%
Radziszewska E., Piwocka K., Bielak-Zmijewska A., Skierski J., Sikora E.
Acta Biochimica Polonica
|
2000
|
tom 47
|
nr 2
UVC-induced apoptotic symptoms such as morphological changes, DNA fragmentation, Bcl-2 and Bax protein expression were examined in primary splenocyte cultures from young (3 months) and old (24 months) rats. The activities of AP-1 and CRE transcription factors in UVC-irradiated splenocytes were also assessed. At 24 h after UVC irradiation 40% of cells derived from young rats were found to be apoptotic, which was twice as much as in splenocytes from old rats. Apoptosis in cells from old rats did not give typical symptoms like a DNA ladder and Bcl-2 protein downregulation, in contrast to splenocytes from young rats. No AP-1 transcription factor activity was found in UVC-irradiated splenocytes from old animals and only a trace activity in splenocytes from young animals. This indicates that, UVC-induced apoptosis in rat splenocytes is practically AP-1 independent and that cells from old rats are less sensitive to UVC irradiation than splenocytes from young rats.
9

Src kinases inhibitor, SU6656, induces differentiation and maturation of megakaryocyte progenitor cells

39%
Kaminska J., Bany-Laszewicz U., Klimczak-Jajor E., Skierski J., Zdebska E., Koscielak J.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
10
Content available remote

Transcriptomic profile of two canine mammary cancer cell lines with different proliferative and anti-apoptotic potential

39%
Krol M., Pawlowski K. M., Skierski J., Rao N. A. S., Hellmen E., Mol J. A., Motyl T.
Journal of Physiology and Pharmacology. Supplement
|
2009
|
tom 60
|
nr 1
95-106
The aim of the study was to identify the genes responsible for the high growth rate and anti-apoptotic potential in selected canine mammary cancer cells. cDNA canine microarrays were used to compare the transcriptome in simple carcinoma CMT-U27 and spindle-cell tumor CMT-U309 cell lines. In CMT-U27 cell line the growth rate (shorter cell cycle), anti-apoptotic potential (higher expression of Bcl-2) was higher and spontaneous and induced apoptosis was lower. Comparison of transcriptomes revealed 333 genes which expression differed similarly. We focused on genes involved in cell proliferation, adhesion and apoptosis, and selected 29 of them. The high growth rate and anti-apoptotic potential in CMT-U27 cells was associated with enhanced expression of genes (at the level of transcripts) involved in Ca2+ signaling pathway (Calmodulin 1, 2, 3 and SPSB2) and growth hormone cellular pathway. The low-proliferative and pro-apoptotic phenotype of CMT-U309 cells was more dependent on TGFß, neuregulin 1 pathways and adhesion-related molecules.
11

Tetrabromobenzotriazole [TBBt] and tetrabromobenzimidazole [TBBz]: selective inhibitors of protein kinase CK2, and their influence on activities of different forms of human CK2, and on the cells

39%
Zien P., Litchfield D. W., Duncan J., Skierski J., Bretner M., Shugar D.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr Suppl.2
12

Effect of adriamycin and gamma-radiation on cultured mammalian cells measured by flow cytometry

39%
Koceva-Chyla A., Distel L., Zaborowski A., Skierski J., Rozga B., Schussler H., Jozwiak Z.
Current Topics in Biophysics
|
1994
|
tom 18
|
nr 2
13

Apoptosis induced by serum deprivation and apoptogenic factors of cellular origin is dependent on bcl-2 and bax expression in L1210 leukaemic cells

39%
Zimowska W., Motyl T., Wareski P., Skierski J., Ploszaj T., Orzechowski A.
Polish Journal of Veterinary Sciences
|
2000
|
tom 03
|
nr 1
Since serum is a source of hormones, growth factors, cytokines and antioxidants being a survival factor for cell culture its withdrawal from the culture medium induces apoptosis in a variety of cell types. In the present study the effect of FCS - deprivation on Bcl-2 protein level, Bax transcript and apoptosis of L1210 leukaemic cells was examined. The possibility of the existence of cellular origin "death factors" was also investigated. A simultaneous flow cytometric analysis of Bcl-2 protein using FITC-conjugated Mo anti-Bcl-2 antibody and the percentage of apoptotic cells by measurement of sub-Gl region in the DNA (stained with DAPI) histogram was performed. Bax transcript was measured using the RT-PCR method with GAPDH as a reference gene. ROS generation was assessed by flow cytometry using the oxidation - sensitive fluorescent marker C-DCDHF-DA. FCS deprivation induced a massive apoptosis of leukaemic cells, reaching 40% of the cell population after 24h. Typical features of apoptosis such as cell shrinkage, condensation of chromatin, pyknosis and fragmentation of nuclei, followed by secondary necrosis (putrosis) were observed using fluorescence microscopy. An increase in intracellular ROS generation by 100% after 24h of serum deprivation was also observed. The apoptotic effect of FCS - deficiency was accompanied by a 32.7% decrease in the number of cells containing Bcl-2 protein (cells with specific anti-Bcl-2 fluorescence), thereby a negative relationship (r=0.98, P<0.01) between the extent of apoptosis and bcl-2 expression was calculated. Since serum deficiency eliminated primarily the cells with the lowest Bcl-2 level, the average Bcl-2 level in the population of surviving cells was even higher than in the control cultures, growing in optimal conditions (10% FCS/RPMI). The conditioned medium (CM), obtained from 24h FCS - deprived L1210 leukaemic cells, contained >10 kDa molecules, which were able to down- regulate Bcl-2, up-regulate Bax, and induce apoptosis. This effect was more visible when >10 kDa CM filtrate was administered to poorly-fed (2% FCS/RPMI) than better-fed (10% FCS/RPMI) cultures. It is concluded that: 1) the susceptibility of L1210 leukaemic cells to serum deprivation - induced apoptosis is dependent on Bcl-2 protein level, and 2) serum - deprived leukaemic cells release >10 kDa molecule/s being inductor/s of programmed cell death, acting through the regulation of Bax/Bcl-2 rheostat.
14

Comparative studies of nuclear DNA content in benign and malignant thyroid lesions

39%
Konarska L., Skierski J., Ellert A., Steinbrich J., Wozniak A., Kalczak M.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 3
Currently available data suggest that DNA aneuploidy is associated with aggressive behavior of and unfavorable prognosis in several malignant human tumors as com­pared with diploid malignancies. However, the diagnostic and prognostic importance of flow cytometric DNA measurements in the case of thyroid neoplasms remains con­troversial. Therefore, the aim of our study was to evaluate utility of DNA index (DI) and proliferative index (PI) in distinguishing benign from malignant thyroid lesions taking into account the possible influence of intra-tumor heterogeneity and tissue preparation mode on DNA flow-cytometry measurements. A retrospective study was performed on 71 paraffin-embedded specimens from 57 patients with benign and malignant thyroid pathologies: 13 colloid goitres, 12 parenchymatous goitres, 19 adenomas and 13 carcinomas. In 14 of 57 cases two sepa­rate specimens taken from different areas of the same lesion were analysed and DNA parameters were compared. Additionally, flow cytometry DNA analysis was parallelly performed on 3 adjacent but differently processed tissue sections (fresh, forma­lin-fixed and paraffin-embedded) taken from each of 26 surgically excised thyroid le­sions. DNA content was also analysed in both fresh and formalin-fixed twin specimens of normal pig thyroid glands (N = 6). We demonstrated that all tumors diagnosed as thyroid carcinomas were associated with abnormal nuclear DNA content although aneuploidy was not found specific to malignant thyroid tumors. Aneuploid samples of benign thyroid lesions exhibited higher proliferative activity, expressed as mean PI values, than diploid ones. In carci­nomas the mean PI values were significantly higher than in benign lesions, independ­ently whether they concerned aneuploid or diploid tissues. Considering intra-tumor heterogeneity, the flow cytometric DNA parameters can be assumed as reproducible despite differences in the mode of tissue fixation and preparation for analysis.
15

Transcriptomic signature of cell lines isolated from canine mammary adenocarcinoma metastases to lungs

33%
Krol M., Polanska J., Pawlowski K. M., Turowski P., Skierski J., Majewska A., Ugorski M., Morty R. E., Motyl T.
Journal of Applied Genetics
|
2010
|
tom 51
|
nr 1
37-50
Metastasis is a final step in the progression of mammary gland cancer, usually leading to death. Potentially, a molecular signature of metastasis can be defined via comparison of primary tumors with their metastases. Currently, there is no data in the literature regarding the molecular portrait of metastases in dogs and only few reports regarding human cancer. This is the first report describing the transcriptomic signature of canine cancer metastatic cells. Two adenocarcinoma cell lines isolated from the canine mammary gland (CMT-W1 and CMT-W2) were compared with cell lines isolated from their lung metastases (CMT-W1M and CMT-W2M) with regards to the following cytometric parameters: cell cycle, ploidy, Bcl-2 expression, susceptibility to induced apoptosis, and transcriptomic profile. Cytometric analyses revealed significant differences in cell cycle and antiapoptotic potential between the examined cells. Using oligonucleotide microarrays, we found 104 up-regulated genes in the metastatic cell line CMT - W1M and 21 up-regulated genes in the primary CMT -W1 cell line. We also found 83 up-regulated genes in the CMT-W2M cell line and only 21 up-regulated genes in the CMT-W2 cell line. Among the up-regulated genes in both metastatic cell lines, we found 15 common genes. These differently expressed genes are involved mainly in signal transduction, cell structure and motility, nucleic acid metabolism, developmental processing, and apoptosis (GHSR, RASSF1, ARF1GAP, WDR74, SMOC2, SFRP4, DIAPH1, FSCN1, ALX4, SNX15, PLD2, WNT7B, POU6F2, NKG7, and POLR2F). Seven of them are involved in a cellular pathway dependent on ghrelin via growth hormone secretagogue receptor (GHSR). Our results suggest that this pathway may be essential for mammary cancer cells to have a metastatic potential.
16

Transcriptomic 'portraits' of canine mammary cancer cell lines with various phenotypes

33%
Krol M., Pawlowski K. M., Skierski J., Turowski P., Majewska A., Polanska J., Ugorski M., Morty R. E., Motyl T.
Journal of Applied Genetics
|
2010
|
tom 51
|
nr 2
169-183
In light of the high incidence of mammary cancer in dogs and completion of the canine genome sequencing, the new possibilities of gene profiling by using DNA microarrays give hope to veterinary oncology. The cell lines isolated from mammary tumors are a valuable tool in developing and testing new pathway-specific cancer therapeutics. Differential cytometric analysis of 6 canine mammary cancer cell lines was performed. We divided cell lines into 3 groups based on their phenotype: 2 lines with high proliferative potential, 2 lines with high antiapoptotic potential, and 2 lines with high metastatic potential. DNA microarray analysis revealed common genes for cell lines of each group. We found that genes encoding the receptors for growth hormone and ghrelin are related to high proliferation rate, while ABR (active BCR-related) and TMD1 (TM2 domain containing 1) genes are related to a high antiapoptotic potential of the cancer cells. Metastatic properties of mammary cancer cells seem to be associated with elevated expression of PGP (P glycoprotein), SEMA3B (semaphorin 3B), and STIM1 (stromal interaction molecule 1).
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.