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The major goal of our study was to demonstrate how to eliminate zinc from the products of waste tire pyrolysis and avoid its environmental circulation. The new tire utilization manner consists of a separate combustion of the pyrolytic char containing 5.1 wt. % zinc and a collection of such obtained ash (10.4% yield) that contains 42 wt. % of zinc. The ash is a good candidate for metallurgical recovery of metallic zinc. The study demonstrates that pyrolytic char, because of its very low BET surface area of 43-60 m2/g, should not be transformed into activated carbon. High surface areas reported in literature (800-910 m2/g) for some pyrolytic chars were achieved after a complex and expensive activation process. Moreover, the study proved that zinc derivatives in tire-driven pyrolytic chars could be released to the environment since they were partly leached in H2O and etched in 0.1 M HCl solution. The study demonstrates that zinc can be eliminated from environmental circulation during tire pyrolysis and proposed an alternative solution to the transformation of pyrolytic char into activated carbon.
Fluorescent in situ hybridization (FISH) was performed in 76 patients referred to our department because of intellectual disability and dysmorphic features that can be related to subtelomeric microaberrations. In all the patients, conventional cytogenetic methods revealed normal karyotype. Four (5.3%) subtelomeric rearrangements were detected by FISH: 2 subtelomeric 1p36 deletions, an unbalanced translocation involving chromosomes 1 and 12 with 1p36 deletion, and a de novo balanced translocation involving chromosomes 19 and 22. Thus, 3 cases of 1p36 subtelomeric deletion were found (3.95%). To confirm subtelomeric rearrangements in 2 patients, comparative genomic hybridization (CGH) was applied. Moreover, 3 cases of polymorphism without phenotypic effects were found: in 2 patients, the polymorphism involved the long arm of chromosome 2 (maternal derivative in both patients), while in the third patient, a polymorphism of the long arm of chromosome 7 was diagnosed. The latter polymorphism was also found in the patient's mother and grandfather.
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