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The genus Murina has a wide geographic distribution and includes about 35 species, but very little information is available on the maternity behaviour of any of them. We conducted a preliminary study of a maternity colony of Murina ussuriensis in warm temperate rain forest of Yakushima, Japan. Day roosts of six nursing females were located by radio-telemetry. During the eight days of the study 18 maternity roost locations were confirmed. The nine roosts that were clearly visible included four types: vertically suspended clusters of dead leaves, tree hollows, a crevice in a root plate and a completely exposed site under a branch. Whenever the same female was located on successive days, she had switched from her previous roost, and none of the roosts was re-used by a radio-tagged female during the study period. The number of individuals recorded roosting together ranged from two (a mother with infant) to 22 individuals. The first emergence of an adult female occurred between 13 and 47 minutes after sunset. Mothers returned to the roost about 30 minutes after their first emergence, repeatedly hovering near the roost, landing and departing again. Some offspring left the roost independently, while non-volant offspring were carried away by an adult, presumably the mother. Various types of social calls were recorded, beginning when the first mother returned to the roost, and then almost continuously until the last individual left the roost. These preliminary results suggest that maternity colonies show very frequent roost-switching, may exhibit fission-fusion behaviour and have an extensive vocal repertoire.
The distribution ofMurina silvatica (Yoshiyuki, 1983) in the Japanese archipelago extends over about 2000 km from north to south. Specimens were obtained from populations in Hokkaido and Yakushima, which are at the northern and southern ends of the range, and from two intermediate locations in Honshu. Measurements of cranial and external morphology were examined for evidence of geographical variation. The results of both multivariate analysis of variance and cluster analysis showed that there was no distinct cline in skull morphology among the Hokkaido, Tohoku and Chubu populations. However, the results of multivariate analysis of variance showed that all measures were significantly greater for the Yakushima population than for the others. Similarly, in a dendrogram of cluster analysis, the Yakushima population formed a cluster that was distinct from the other populations. However, as the difference between the Yakushima population and the other populations was less than the variation found within the Hokkaido, Tohoku and Chubu populations, morphological divergence of the Yakushima population was attributable to intraspecific variation. The island of Yakushima is the most isolated of the four locations and the morphological divergence of this population may be associated with its relative geographic isolation.
Previously, braincase shape has been the most useful character for species diagnosis of the Myotis mystacinus group members in Hokkaido, Japan. However, this character is difficult to apply in live individuals. Recently, the different patterns of venation in the tail membrane were described for species diagnosis of M. ikonnikovi and M. mystacinus (actually M. brandtii gracilis in Japan). We evaluated this new method in these two cryptic species in Hokkaido, Japan, based on molecular analysis. Our results revealed complete congruence between haplotypes of the mtDNA cytochrome b gene and the patterns of tail membrane venation in these two species. Thus, tail membrane venation pattern should be regarded as an unambiguous diagnostic character to distinguish M. ikonnikovi and M. b. gracilis under field conditions in Hokkaido. Further, since our field sampling was extensive, we suggested that only two species in the M. mystacinus group, M. ikonnikovi and M. b. gracilis occur in Hokkaido, and concluded that the occurrence of other cryptic species in the group in Hokkaido is unlikely.
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