Wyniki wyszukiwania

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Morphological and molecular evaluation of native Scots pine (Pinus sylvestris L.) populations from South-Eastern Europe

100%

Toth E. G., Pedryc A., Hohn M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2013

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tom 55

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nr 1

2

S-genotype Characterization of several hungarian apricot varieties

100%

Halasz J., Ruthner S., Bekefi Z., Pedryc A.

Acta Physiologiae Plantarum

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2004

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tom 26

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nr 3 suppl.

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Genetic characteristics of the Carpathian endemic Syringa josikaea: regional structuring of small and isolated populations

100%

Lendvay B., Pedryc A., Hohn M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2013

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tom 55

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nr 1

4

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Transgenic callus culture establishment, a tool for metabolic engineering of Rhodiola rosea L.

88%

Mirmazloum I., Forgacs I., Zok A., Pedryc A., Gyorgy Z.

Acta Scientiarum Polonorum. Hortorum Cultus

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2014

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tom 13

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nr 4

Agrobacterium tumefaciens EHA101 (pTd33) strain carrying uidA (GUS) reporter gene was used in model experiments on roseroot callus transformation. The T-DNA of pTd33 binary vector plasmid harbors nptII gene conferring resistance to kanamycin, and a uidA reporter gene, encodes the ß-glucuronidase enzyme. Roseroot seeds were sterilized and germinated on half strength MS media of which 70% germinated without any pretreatment. Calli were obtained from leaf segments of the in vitro grown seedlings. Calli was grown on solid MS medium supplemented with 1 mg l⁻¹ NAA and 0.5 mg l⁻¹ BAP. Different types of calli were obtained of which the green and compact type was chosen for transformation experiments. After co-cultivation with agrobacteria, calli were transferred to the same medium supplemented with 20 mg l⁻¹ kanamycin, 200 mg l⁻¹ carbenicillin and 300 mg l⁻¹ claforan with antioxidants (Polyclar and DTE) for selection. GUS test using a titron buffer was applied for monitoring the transformation of the calli. DNAs of 20 individual samples was extracted and subjected for PCR analysis proved the stable transformation in all of the taken samples by amplifying the nptII gene fragment. The method introduced here can be a tool for inserting and over-expressing the genes encoding for hypothesized enzymes to be involved in the biosynthesis of pharmaceutically important bioactive molecules of roseroot and therefore facilitating the applications for callus culture of roseroot in different bioreactor systems for pharmaceutical productions.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica. Supplement

1 Acta Physiologiae Plantarum

1 Acta Scientiarum Polonorum. Hortorum Cultus

4 Pedryc A.

2 Hohn M.

1 Bekefi Z.

1 Forgacs I.

1 Gyorgy Z.

1 Halasz J.

1 Lendvay B.

1 Mirmazloum I.

1 Ruthner S.

1 Toth E. G.

1 Zok A.

1 2014

2 2013

1 2004

Morphological and molecular evaluation of native Scots pine (Pinus sylvestris L.) populations from South-Eastern Europe

S-genotype Characterization of several hungarian apricot varieties

Genetic characteristics of the Carpathian endemic Syringa josikaea: regional structuring of small and isolated populations

Transgenic callus culture establishment, a tool for metabolic engineering of Rhodiola rosea L.