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Allium tuncelianum (Tunceli garlic) is a single cloved important threatened endemic geophyte of high commercial importance that grows scattered on hot temperate mountains under oak trees in the eastern Turkish province of Tunceli. Tunceli garlic is used diversely in salads, foods and in pharmaceutical industry. They also offer a rich choice for their use in rock gardens and landscaping. There is need to develop appropriate agronomic practices for its propagation outside its habitat. In line with this objective, the study optimized planting time (1st September, 15th September and 1st October) of A. tuncelianum on mild alkaline soils under semi-arid climatic conditions of Diyarbakir, Turkey. The results of the study showed positive gain in morphological characteristics such that bulb diameter of the plants changed between 3.43 to 4.60 cm, bulb circumference ranged 14.4 to 16.1 cm and bulb weight had a range of 35.9 and 46.3 g. These results underscore positive effects of early and mid September plantings of Tunceli garlic outside its habitat under semi-arid conditions for economic production with early harvest.
Hyssop (Hyssopus officinalis L.) is an aromatic and medicinal perennial herb native to Southern Europe and temperate regions of Asia. The study aimed to determine the effect of different plant parts harvested at different flowering stages: pre-flowering, full-flowering and post-flowering, in some agronomical characteristics, essential oil yield and components of hyssop plant in semi-arid climatic conditions of South Eastern Anatolia, Diyarbakir, Turkey. The results showed that both flowering stages and plant parts had important effects on fresh, dry herbage, dry leaf yield of hyssop, as well as essential oil content. It was concluded that under semi-arid climatic conditions, hyssop plant could be grown successfully in conformity to the limits of ISO standards and harvest from top parts of the plants at full flowering stage could be preferred for maximum dry leaf yield and essential oil contents.
Hyacinthus genus is an important group of ornamental plants that bear white, yellow, pink, red or purple coloured flowers. It has about 2000 species spread around the world that are grown commercially. Although, plant occurs naturally in Turkey yet efforts have not been made to adapt it for open field cultivation. There is need to transfer and establish these plants from wild to fields for commercial use through in vitro and ex vitro approaches, that will help local economy profitably. This study reports in vitro culture of Hyacinthus orientalis L. subsp. orientalis; using fruits containing immature zygotic embryos cultured on MS medium containing varying concentrations of Thidiazuron (TDZ) with and without 0.2 mg l-1 naphthaleneacetic acid (NAA) supplemented with 20 or 40 g l-1 sucrose. The study also reports induction of bulblets on tender leaf sheaths on MS medium containing different concentrations of benzylaminopurine (BAP) + 0.1 mg l-1 NAA supplemented with 30 g l-1 sucrose. The maximum bulblet regeneration (40%) with 31.33 bulblets/explant was noted on MS medium containing 0.15 mg l-1 TDZ supplemented with 40 g l-1 sucrose. Whereas, the best bulblet regeneration on tender leaf sheath explants was noted on 1.5 mg l-1 BAP + 0.1 mg l-1 NAA with 2.97 bulblets per explant of 0.55 cm bulb diameter and 1.20 leaves per bulblet. These bulblets were cultured singly on MS medium containing 20 mg l-1 GA3 (Gibberellic acid) + 50 g l-1 sucrose and attained a diameter of 0.75–1.00 cm in 30 days time. The bulbs regenerated on both explants were successfully rooted and acclimatised in plant growth chamber using peat moss followed by their transfer to open field conditions.
The study aimed to determine agronomic characteristics and essential oil components of different basil ecotypes in semi-arid climatic conditions of South Eastern Anatolia, Diyarbakir, Turkey. Two-year harvest data about fresh and dry herb yield, dry leaf yield, essential oil content and its components from the plants of year 2015 and 2016 was analyzed in this study. Essential oil components were detected by gas chromatography/ mass spectrometry (GC/MS). The resulting outcomes demonstrated that both ecotypes and harvests had important effects on fresh and dry herb, dry leaf yield and essential oil contents of sweet basil. The highest dry leaf yield was noted from green leafy ecotypes and from second harvest stage. Twenty-three constituents were detected in the essential oil of O. basilicum ecotypes. The main components of basil essential oil were linalool, methyl chavicol, neral, geranial and methyl cinnamate that differed according to ecotypes and harvests during experimental years 2015 and 2016. Purple leafy basil ecotypes were determined as linalool rich, while greenish leaf ecotypes were abundant in methyl chavicol. Silbe – green ecotype contains higher neral and geranial levels than the other ecotypes. It was concluded that basil plant could be grown successfully and harvested two or more times to prefer for maximum dry leaf yield and essential oil contents under semi-arid climatic conditions.
Lemon verbena is cultivated mainly due to the lemon-like aroma emitted from its leaves that are utilized for most purposes. The chemical composition of the essential oil of lemon verbena was analyzed by GC/MS in July at pre flowering, in September at full flowering and in end of October at post flowering period. The in vitro antimicrobial activity of the essential oil, extracted from Lippia citriodora was tested against laboratory control strains belonging to the American Type Culture Collection (Maryland, USA) four Gram (+) and Gram (-) bacteria using the disc diffusion test. Antioxidant activity of the sample was determined by 1,1-diphenil-2- -picrly-hydrazil (DPPH) assay. Well-known antioxidant compounds such as ascorbic acid and α-tocopherol were used as standard. Results showed, among different harvest periods high fresh and dry herbage and dry leaf yields per plant were obtained from full flowering stage. Harvest periods were found to have a significant effect on the content of essential oil and the highest amounts of limonene, neral and geranial and measured as 31.15, 11.92 and 15.53%, respectively at full flowering stage. In all samples, the main constituents were limonene, neral and geranial constituting 46.03%–58.59% of the total essential oil yield depend on development stages. Lemon verbena essential oil was found to have antibacterial activity especially against Gram (+) microorganisms. In DPPH system, the moderate radical scavenging activity was exhibited. Therefore, L. citriodora has potential to be used as a natural antioxidant and antimicrobial agent in food processing.
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