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Zastosowanie reakcji PCR w diagnostyce nosowki psow

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Winiarczyk S., Adaszek L., Dziduszko A., Gradzki Z., Madany J., Skrzypczak M.

Medycyna Weterynaryjna

2006

tom 62

nr 07

840-841

The aim of the study was to estimate the utility of the PCR method in diagnosing canine distemper. A group of 4 healthy, vaccinated dogs (Canivac F Biowet Pu³awy) and a group 14 sick animals having symptoms of distemper were included in the experiment. Specimens of blood from all dogs were collected and leucocytes were isolated, RNA extracted and reverse transcription carried out. The obtained c DNA was used in RT-PCR. Genetic material of the virus in the leucocytes of all the vaccinated animals was detected using RT-PCR between day 6-19 and by nested PCR between days 2-24 following immunization. RT-PCR was positive in 3 dogs of the group of sick animals and nested PCR in 9 additional dogs.

Ograniczanie wyników

1 Medycyna Weterynaryjna

1 Adaszek L.

1 Dziduszko A.

1 Gradzki Z.

1 Madany J.

1 Skrzypczak M.

1 Winiarczyk S.

1 2006

Wyniki wyszukiwania

Zastosowanie reakcji PCR w diagnostyce nosowki psow