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Analysis of multiple regression of data obtained on 347 donor cows revealed a statistically significant interaction (P<0.001) between FSH drugs and: 1- the age of cows. 2 - month of examination and the number of transferable embryos and month of their yields. 3 – the number of superovulations per cycle and the number of transferable embryos.
Подвергали бисекции поздние бластoциды скота. Операции проводили сквозь прозрачную оболочку, а полученные „голые” полoвинки трансплантировали рррррррррряо’аон синхронизированным реципиенткам. 29 реципиенткам перенесли по одной половинке, получив 7 телят, в том пару монозиготических близнецов. Второй группе реципиентoк, состоящей из 29 голов, перенесли по 2 половинки. Получили 8 родоз, из чего 4 были близнецовыми. В контрольной группе 72 реципиенткам ввели по 1 целому зародышу, получив 26 отелов.
The aim of this study was determining influence of some factors on pregnancy results in recipient heifers of fresh embryos. The analysis was carried out on 1641 animals (1847 embryo transfer interventions of an average age of 16-26 months. The mean pregnancy rate in heifers was 46.4% and was dependent (p<0.001) on which the quality of embryos, kind of medium used to embryo collection, herds from recipients descended, number of interventions on one recipient, stage of embryo development, kind of medium to embryo culture and conservation, horn of uterus and the month. The best results were noted in recipients which received very good than poor quality embryos (60 resp. 26.1%). There was a big difference in pregnancy results between recipients coming from different herds (23 resp. 60%). When heifers were used as recipients two or more times the pregnancy results were lower than in heifers used only once (47.1 resp. 43.6% and 19.2%). A significant influence on pregnancy results was also the kind of medium used for the cultivation of embryos (Dulbeco (Menezo) or Dulbeco (Sigma) - 50.2 or 56.6% resp. OCM (Imperial - 26.8%). Likewise results of pregnecies were highest in October (62.2%) and lower in summer months (July 33.3%).
Seasonal differences were found in the rate of fertilisation in 1641 cows implanted with fresh embryos. The rate of fertilisation was higher (P<0.01) than the respective mean values in October, January, April, June and February and lower (P<0.01) in July and November. However, the rate of fertilisation differed insignificantly in cows implanted with frozen embryos. The month of superovulation significantly affected the number of produced embryos and oocytes, embryos suitable for transfer and total embryos; the number of embryos was lowest in Summer, and highest in January, February, and March.
The distribution and the number of the T. spiralis larvae per 1 g of muscle were determined in rats experimentally infested at the rate of 200, 500 or fed meat containing the larvae. The examinations were also carried out on pigs fed 200, 1000 and 10 000 larvae. The intensity of larvae invasion and distribution depended on the dose used for infestation. The highest number of the larvae in rats and pigs infested with lower doses of T. spiralis was found in the tongue muscles, masseter, femur and diaphragm. In animals infested with medium-sized inocula (rats with 500 and pigs 1000 larvae) the highest number of the larvae was noted in the intercostal muscles, in the masseter in the transversal muscles of the abdomen, in the biceps muscle of the thingh and in the diaphragm. In pigs infested with 10 000 larvae and in rats fed infested meat the highest number of the T. spiralis larvae was found in the diaphragm. Numerous larvae were also noted in the tails and oesophagi of the animals under study.
One hundred and sevently four young calves with the sins of bronchopneumonia cured traditionally with antibiotics (penicillin plus streptomycin) were treated additionally with levamisole (2 mg/kg IM) or nitrogen mustard (chlormethine hydrochloride) administering 2 mce/kg IV or 4 mcg/kg IM. There was found a significant improvement of animals’ state and shortening the time of recovery. No influence on the level of immunoglobulin was found.
Upon inducing 166 super ovulations the effect of FSH-P (Schering) and Folicotropin (Spofa) on the effects of super ovulation and number of embryos was estimated in Limousine (60 super ovulations), Aberdeen-angus (43 super ovulations) and black and white (63 super ovulations) cows using an analysis of multipartial regression. The effect of super ovulation measured by the number of luteal corpuscles on ovaries depended on the breed of the cow (p=0.0145) and the preparativ used (p=0.0195). The best results were obtained after the application of Folicotropin. The number of the obtained cells was comparable after the use of the two examined preparatives, whereas the number of embryos useful for transfer was higher in the meat type of cows than in the black and white breed (p=0.0130). In conclusion, the value of the two examined preparatives for embryo transfer is equal. In choosing a preparative differences in the sensitivity of cows to gonadotrophins should be borne in mind.
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