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Charophytes possess the capacity to store large amounts of various elements, but until today no studies about known methods of determining CaCO₃ and elements in charophytes have been conducted. Because of the large and varying amounts of CaCO₃, it is difficult to estimate the actual element content in dry mass. Simple comparisons of elements in charophyte plant tissue or between charophytes and higher plants are impossible. The main aim of this study was to investigate through laboratory experiments content of carbonates and elements in charophyte tissue using different methods of decomposition (mineralization). The proposed protocol: determination of calcium carbonate content in plants, determination of elements present in the plant dry mass, and calculation of the element content in the plant dry mass as described.
The distribution and ecology of Chara braunii Gmellin 1826 in Poland is described, based on herbaria collections, literature data and own investigations. Maps showing the distribution of Ch. braunii are presented, as well as a list of localities with brief descriptions of the habitats. Additionally, some remarks on the distribution limit in the northern hemisphere are given.
Allium sibiricum is one of the rarest plant species in the Sudetes and Carpathians. Inter simple sequence repeat DNA (ISSR) and morphological analyses were conducted to study the biogeographical relationships between geographically disjunctive populations of A. sibiricum in the Carpathians and Sudetes. The results clearly differentiated the Carpathian and Sudetes populations, but also showed a relatively high level of genetic similarity in specimens within certain groups of the Sudetes and Carpathian populations. The plants collected in the Karkonosze probably belong to a mountain form of A. schoenoprasum morphotype C which inhabits natural sites there. In contrast, the study found morphologically and genetically different plants inhabiting Pilsko Mt in the Carpathians. The plants from the Carpathians were present in scattered sites probably due to the activity of Vlach shepherds and the formation of large pastures. The species enlarged its local range due to this type of anthropopressure and likely hybridized with the cultivated Allium plants introduced by the shepherds. This may indicate that the populations on Pilsko Mt are of a partly anthropogenic character.
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