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The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea

100%

Luo Y., Tang Y., Xia Q., Liu J.

Cellular and Molecular Biology Letters

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2007

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tom 12

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nr 4

595-603

Endothelin (ET), originally characterized as a vasoconstrictive peptide, has been found to have many different biological functions, including acting as a local hormonal regulator of pressure, fluid, ions and neurotransmitters in the inner ear. The objective of this study was to examine and quantify the mRNA expression of the endothelin type A and B receptors (ETAR and ETBR) in the strial vascularies (StV) and non-strial tissues (NSt) of the cochlear lateral wall using the real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) technique. The mouse tissue samples were harvested and RNA was extracted. RT was performed to obtain cDNA, and then the mRNA expression of each gene was measured via real-time PCR. We found that both receptor subtypes were expressed in the cochlear lateral wall, with a predominance of ETAR over ETBR. We showed that the mRNA expression of the two receptor subtypes was higher in the StV with a 1.8 times higher level of ETAR and an 8.1 times higher level of ETBR mRNAs than in the adjacent NSt of the lateral wall tissue. This study shows the existence and the quantity of ET receptor subtypes in the StV and NSt of the mouse cochlea. Our results suggest that an endothelin-mediated response via two different receptors, ETAR and ETBR, may play an important role in the physiological functions of the cochlear lateral wall by maintaining the homeostatic environment of the cochlea.

2

Characterization of endolithic culturable microbial communities in carbonate rocks from a typical karst canyon in Guizhou (China)

100%

Tang Y., Cheng J.-Z., Lian B.

Polish Journal of Microbiology

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2016

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tom 65

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nr 4

The endolithic environment is a ubiquitous habitat for microorganisms and a critical interface between biology and geology. In this study, a culture-based method and the phylogenetic analysis based on 16S rRNA and internal transcribed spacer (ITS) sequences were used to investigate the diversity of endolithic bacteria and fungi in two main types of carbonate rocks (namely dolomite and limestone) from Nanjiang Canyon in Guizhou karst area, China. The results of bacterial diversity indicated that all bacteria isolated from dolomite and limestone rocks were divided into 4 bacterial groups, including Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. For these two kinds of rocks, Proteobacteria was the first dominant group, and Gammaproteobacteria occupied the greatest proportion which might be closely related to Pseudomonas in phylogeny to be the most dominant genera after isolation. Actinobacteria and Bacillus bacteria were also widespread in these two kinds of rock environments. There were only 9 and 8 strains of fungi isolated from dolomite and limestone respectively, which all belonged to Ascomycota. To the best of our knowledge, this is the first report on diversity of endolithic culturable bacteria and fungi in carbonate rocks in Guizhou karst region. These microorganisms may play an important and unprecedented role in the carbonate rock weathering during the long history of geological evolution.

3

Study of the clogging properties of mixed leachate

88%

Xie M., Liu D., Tang Y., Feng M., Yi X.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 5

4

The effects of litter layer and soil properties on the soil-atmosphere fluxes of greenhouse gases in karst forest, Southwest China

76%

Cheng J., Lee X., Zhou Z., Wang B., Xing Y., Cheng H., Tang Y.

Polish Journal of Ecology

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2013

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tom 61

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nr 1

Temporal variation is a major source of the uncertainty in estimating the fluxes of the greenhouse gases (GHGs) in terrestrial ecosystems, and the GHG fluxes and its affecting factors in the karst region of southwest China remains weakly understood. Using the static chamber technique and gas chromatography method, the CO₂, CH₄ and N₂O fluxes were carried out between 9 and 11 a.m. at 15 day intervals from June 2008 to May 2009 in a Pinus massoniana forest. Two treatments were chosen for this study: undisturbed (soil with litter layer) and disturbed (surface litter removal). Both treatments were found to be the net source of atmospheric CO₂ and N₂O, but a sink of atmospheric CH₄. The seasonality of soil CO₂ emission coincided with the seasonal climate pattern, with high CO₂ emission rates in the hot-wet season and low rates in the cool-dry season. In contrast, seasonal patterns of CH₄ and N₂O fluxes were not clear, although higher CH₄ uptake rates were often observed in autumn and higher N₂O emission rates were often observed in spring (dry-wet season transition). The litter was active in GHG fluxes, and removal of the litter layer reduced soil CO₂ emission (17%) and increased CH₄ uptake (24%) whereas N₂O fluxes were not affected distinctly in the pine forest, indicating that litter layer had an important effect on C exchanges. In the pine forest, soil CO₂ emissions and CH₄ uptakes correlated significantly with soil temperature (r²= 0.87, P <0.01; r²= 0.34, P <0.05, respectively), but had no significant relationship with soil moisture. And there was a significant correlation between CH₄ flux and NH₄⁺-N (r²= 0.39, P < 0.05) and soil inorganic N (r²= 0.48, P <0.05), but no significant correlation was found between CH₄ flux and NO₃⁻-N. Moreover, we found a significant negative logarithmic correlation between N₂O flux and soil NO₃⁻-N concentration (r²= 0.41, P <0.05), and the relationship between CO₂ emission and soil inorganic N content (r²= 0.35, P < 0.05). These results suggested that soil temperature and mineral N dynamics largely affected the temporal GHG exchanges between forest soil and atmosphere.

5

Enriched environment increases the total number of CNPase positive cells in the corpus callosum of middle-aged rats

64%

Zhao Y.-Y., Shi X.-Y., Qiu X., Zhang L., Lu W., Yang S., Li C., Cheng G.-H., Yang Z.-W., Tang Y.

Acta Neurobiologiae Experimentalis

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2011

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tom 71

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nr 3

It had been reported that enriched environment was beneficial for the brain cognition, neurons and synapses in cortex and hippocampus. With diffusion tensor imaging (DTI), several studies recently found the trained-induced larger corpus callosum. However, the effect of enriched environment on the oligodendrocytes in corpus callosum has not been explored with the unbiased stereological methods. In current study, the effect of enriched environment on the total number of 2',3'- cyclic nucleotide 3'-phosphodiesterase (CNPase) positive cells in middle-aged rat corpus callosum was investigated by means of immunohistochemical techniques and the unbiased stereological methods. We found that, when compared to standard rats, the spatial learning capacity of enriched-environment rats was significantly increased. The total number of the CNPase positive cells in the corpus callosum of enriched-environment middle-aged rats was significantly increased when compared to standard rats. The present study provided, to the best of our knowledge, the first evidence of environmental enrichment-induced increases in the total number of CNPase positive cells in the corpus callosum of middle-aged rats.

6

Transcriptome profiles reveal cold acclimation and freezing tolerance of susceptible and tolerant hulless barley genotypes

64%

Yuan1,2 · Xingquan Zeng1,2 · Zhihao Ling3 · Yawei Tang1,2 · Nyima Tashi1,2 H., Zeng X., Ling Z., Wei Z., Wang Y., Zhuang Z., Xu Q., Tang Y., Tashi N.

Acta Physiologiae Plantarum

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2017

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tom 39

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nr 12

7

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Biological characteristics of a new antibacterial peptide and its antibacterial mechanisms against Gram-negative bacteria

52%

Pei Z., Ying X., Tang Y., Liu L., Zhang H., Liu S., Zhang D., Wang K., Zhang D., Kong L., Gao Y., Ma H.

Polish Journal of Veterinary Sciences

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2018

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tom 21

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