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1

Cocaine- and amphetamine-regulated transcript-like immunoreactivity (CART-LI) in intramural ganglia of porcine urinary bladder trigone

100%
Zacharko-Siembida A., Arciszewski M. B.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 10
Although the expression pattern of cocaine- and amphetamine-regulated transcript (CART) has been studied in several porcine tissues, it is notable that no data are available on CART expression in the lower urinary tract. In order to map and determine the neurochemical code of CART-like immunoreactivity in the intramural ganglia of the porcine urinary bladder trigone, cryostat sections were immunohistochemically double-stained for CART and HuC/D, as well as for substance P (SP), calbindin, somatostatin and pituitary adenylate cyclase-activating peptide (PACAP). In the ganglia of the urinary bladder trigone, immunoreactivity to CART was detected both in numerous nerve fibres and in minor subpopulation of HuC/D-positive neuronal cell bodies (2.7 ± 0.8%). Neither CART-immunoreactive (IR) nerve fibres, nor CART-IR ganglionic neurons showed simultaneous expression of somatostatin, calbindin and SP. In a substantial proportion of CART-IR neurons (but not nerve fibres) co-localization with PACAP was found. This data suggest that CART present in nervous structures of the porcine urinary bladder may have a role in the parasympathetic regulation of several urinary bladder functions.
2

Red kidney bean (Phaseolus vulgaris) lectin stimulation increases the number of enterochromaffin cells in the small intestine of suckling piglets

81%
Zacharko-Siembida A., Velverde Piedra J. L., Strzalka B., Arciszewski M. B.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 2
The quantities and distribution patterns of serotonin-immunoreactive (serotonin-IR) enterochromaffin cells (EC) were studied immunohistochemically in the small intestine of suckling piglets stimulated with red kidney bean lectin, and in non- stimulated, control animals. The co-expression patterns of serotonin with somatostatin (SOM) or corticotropin releasing-factor (CRF) were also studied. After the lectin treatment, the increased numbers of EC were noted in the duodenum of experimental animals. Lectin stimulation did not change the proportions of EC in the jejunum and ileum. In the duodenal epithelium of the lectin-stimulated piglets, the vast majority of serotonin-IR EC were distributed at the basis of crypts. After the lectin administration, the proportions of serotonin-IR/SOM-IR EC were statistically similar in all sections of the small intestine. No upregulation of CRF was found in duodenal, jejunal, and ileal EC of lectin-treated animals. The findings demonstrated that red kidney bean lectin increased the serotonin reservoir in the duodenum, and thus may be an effective stimulant of the gut maturation in suckling mammals.
3
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Changes in expression of calbindin 28 kDa in the small intestine of red kidney bean (Phaseolus vulgaris) lectin-treated suckling piglets

81%
Zacharko-Siembida A., Valverde Piedra J. L., Arciszewski M. B.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 2
Calbindin (CB) is a calcium binding protein playing a role in calcium uptake and anti-apoptotic cellular protection. The expression of CB was immunohistochemically studied in the small intestine of normal and red bean kidney lectin-treated suckling piglets. In the duodenum and jejunum (but not ileum) of lectin-treated animals overexpression of CB was noted in chromogranin A-immunoreactive (CgA-IR) neuroendocrine (NE) cells. In both control and experimental group a small population of CB-IR NE cells exhibited the presence of somatostatin (but not serotonin, histamine or CRF). After the lectin treatment, an increased (however not statistically significant) immunoreactivity to CB was found in a small subpopulation of neurons of outer submucous (but not inner submucous and myenteric) plexus. It is suggested that there is a functional interaction between lectin administration and CB-expression in the porcine small intestine. Future studies will be needed to clarify this processes.
4
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Immunohistochemical characterization of the jugular (superior vagal) ganglion in the pig

81%
Sienkiewicz W., Dudek A., Zacharko-Siembida A., Marszalek M.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 2
The study was carried out on three 4-month old female pigs. All the animals were deeply anesthetized and transcardially perfused with 4% buffered paraformaldehyde (pH 7.4). Left and right superior vagal ganglia (SVG) were collected and processed for immunofluorescence labeling method. The preparations were examined under a Zeiss LSM 710 confocal microscope equipped with adequate filter block. Neurons forming SVG were round or oval in shape with a round nucleus in the center. The majority of them (52%) were medium (M) (31-50 μm in diameter) while 7% and 41% were small (S) (up to 30μm in diameter) or large (L) (above 50 μm in diameter) in size, respectively. Double-labeling immunofluorescence revealed that SVG neurons stained for CGRP (approx. 57%; among them 37%, 9% and 54% were M, S and L in size, respectively), SP (14.5%; 72.4% M, 3.4% S, 24.2% L), VACHT (26%; 63% M, 24% S and 13% L ), GAL (14%; 57% M, 29% S, 14% L), NPY (12%; 53% M, 12% S, 35% L), Met-Enk (5%; 40% M, 6% S and 54% L), PACAP (15%; 52% M, 24% S and 24% L),VIP (6.3%; 67% M, 8% S and 25% L), and NOS-positive (6%; 31% M and 69% L). The most abundant populations of intraganglionic nerve fibers were those which stained for CGRP or GAL, whereas only single SP-, PACAP- or Met-ENK-positive nerve terminals were observed.
5
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Immunohistochemical study on the expression of biologically active substances in the endocrine pancreas of the European bison

61%
Mozel S., Szymanczyk S., Krzysiak M., Puzio I., Zacharko-Siembida A., Arciszewski M. B.
Polish Journal of Veterinary Sciences
|
2020
|
tom 23
|
nr 3
6
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Colocalization pattern of cocaineand amphetamine-regulated transcript peptide and parvalbumin immunoreactivity in the hippocampus proper of the chinchilla

61%
Szalak R., Matysek M., Kukula-Koch W., Rycerz K., Zacharko-Siembida A., Arciszewski M. B.
Polish Journal of Veterinary Sciences
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2020
|
tom 23
|
nr 1
7

Expression of cocaine- and amphetamine-egulated transcript in the pancreas of the European bison

61%
Mozel S., Matysek M., Zacharko-Siembida A., Szalak R., Krzysiak M., Arciszewski M. B.
Medycyna Weterynaryjna
|
2019
|
tom 75
|
nr 11
Cocaine- and amphetamine-regulated transcript (CART) is a recently discovered neuropeptide thought to mainly act in most laboratory mammals and humans as anorexigenic factor. The expression of CART in wild living animals is barely known. In the present study immunohistochemical stainings were applied to identify CART-immunoreactive (IR) structures in the pancreas of European bison. Antibodies against neuronal marker Hu C/D were used to visualize intrapancreatic neurons. The expression of CART was detected in approx. 75% of Hu C/D-IR intrapancreatic neurons which may thus also act as interneurons. Additionally, in most intrapancreatic ganglia single CART-IR non-varicose nerve fibers running between neurons were found. Pancreatic blood vessels as well as intralobular ducts were sparsely innervated with CART-IR nerve fibers. Moderately numerous CART-IR nerve terminals were found to innervate the pancreatic endocrine and exocrine tissue. None of islet endocrine cells showed the expression of CART. No presence of CART-IR neuronal elements were found in external connective tissue capsule and septa penetrating inside to the organ. Our study is the first to outline the presence of some differences in CART-ergic innervation pattern of the pancreas between domestic and wild mammals. The lack of CART-IR endocrine islet cells in the pancreas of European bison is an interesting finding, nevertheless its significance is largely unknown at the moment and needs to be further investigated.
8
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Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig

61%
Zacharko-Siembida A., Matysek M., Szalak R., Radlinska A., Obszanska K., Arciszewski M. B.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 1
Cocaine- and amphetamine-regulated transcript (CART) peptides are widely expressed not only in the brain but also in numerous endocrine/neuroendocrine cells as well as in neurons of the peripheral nervous system. The present study investigated the distribution patterns of CART-like immunoreactivity in the pelvic plexus (PP) of the female pig. The co-expression of CART with principal neurotransmitter markers: choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), serotonin (5-HT) or biologically active neuropeptides: pituitary adenylate cyclase-activating polypeptide (PACAP), substance P (SP), calbindin was analyzed using double immunohistochemical stainings. Amongst neurons immunopositive to Hu C/D panneuronal marker as many as 4.1 ± 1.2% in right and 4.4 ± 1.6% in left pelvic ganglia were found to express CART. The vast majority of CART-IR ganglionic neurons were predominantly small in size and were evenly scattered throughout particular ganglia. Immunoreactivity to CART was also detected in numerous nerve terminals (which frequently formed pericellular formations around CART-negative perikarya) as well as in numerous nerve fibres within nerve branches interconnecting the unilateral pelvic ganglia. Immunohistochemistry revealed that virtually all CART-IR neurons were cholinergic in nature and CART-IR basket-like formations frequently encircled TH-positive/CART-negative perikarya. None of CART-IR ganglionic neurons showed immunoreactivity to SP, PACAP, 5-HT or calbindin. CART-IR nerve fibres ran in a close vicinity to serotonin-containing cells or faintly labelled SP-expressing neurons. On the other hand, PACAP-IR, SP-IR (but not 5-HT-positive) nerve terminals were found to run in close proximity to CART-IR neurons. Our results indicate that: 1) CART present in PP may influence the activity of pelvic ganglionic neurons/SIF cells, 2) PP should be considered as a potential source of CART-like supply to pelvic viscera and 3) functional interactions between CART and SP or PACAP are possible at the periphery.
9
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Effect of feeding with bilberry fruit on the expression pattern of alphaCaMKII in hippocampal neurons in normal and diabetic rats

61%
Matysek M., Mozel S., Szalak R., Zacharko-Siembida A., Obszanska K., Arciszewski M. B.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 2
αCaMKII, widely occurring in the central nervous system, plays a significant role in cognitive processes. It is well known that diabetes is a risk factor that may trigger brain atrophy, cognitive dysfunction and finally lead to memory loss. Antioxidants richly present in bilberry fruits are believed to have significant effects on diabetes-related brain dysfunctions mainly due to their abilities to modulate neurotransmitter release that lead to reduction of the negative impact of free radicals on cognitive processes. The aim of the present research was to immunohistochemically investigate the expression patterns of αCaMKII in hippocampal neurons from non-diabetic, diabetic and diabetic rats fed with an extract of bilberry fruit. The obtained results show that in comparison to the control group, in diabetic rats hippocampal neurons immunoreactive (ir) to αCaMKII were swollen and the lengths of the neuronal fibres were reduced. Further study shows that in diabetic rats fed with bilberry fruit, αCaMKII-positive nerve fibres were significantly longer when compared to the groups of diabetic and control rats. Additionally, we observed statistically significant changes in the average larger diameter of αCaMKII-ir hippocampal neurons between groups of diabetic rats (with vs. Without supplement of bilberry fruit). The results of the present work suggest that antioxidants present in bilberry fruits influence the morphology of and possibly exhibit beneficial and neuroprotective effects on hippocampal neurons during diabetes. It is likely that changes in the appearance of αCaMKII-expressed hippocampal neurons may reflect the diabetes-evoked rise in Ca²⁺ level in the cerebral nerve terminals. The present research extends our knowledge of preventive mechanisms for cognitive dysfunctions occurring in the brain during diabetes.
10

Expression of serotonin, somatostatin, and glucagon-like peptide 1 (GLP1) in the intestinal neuroendocrine cells of pigs fed with population rye type and hybrid rye type grains

51%
Zacharko-Siembida A., Arciszewski M. B., Valverde Piedra J. L., Tomaszewska E., Szymanczyk S., Muszynski S., Dobrowolski P., Mozel S., Schwarz T.
Medycyna Weterynaryjna
|
2019
|
tom 75
|
nr 10
Neuroendocrine cells (NEC) are a cell population in the gastrointestinal tract that plays a role in the regulation of the digestion process, satiety and nutrient homeostasis. NE cells express a variety of bioactive hormones that can undergo changes in response to different luminal stimuli, including multiple components, which are present in the diet. In recent years, a modern (hybrid) type of rye grain has been introduced to feed industry. The goal of the present study was to determine immunohistochemically whether the feeding of the pigs with population and hybrid rye grains may evoke adverse changes in the small and large intestines in terms of the expression of serotonin, glucagon-like peptide 1 (GLP1) and somatostatin. Feeding animals with population and hybrid rye grains resulted in a slight increase in serotonin-positive NE cells in the small intestine (but not in the large intestine). After feeding animals with population rye (but not with hybrid rye) grains, there was a decrease in the small intestine GLP1-immunoreactive NE cells was found. No changes in the expression of GLP1 were found in the large intestine of experimental animals. The numbers of somatostatin-IR NEC in the small and large intestines were not affected by feeding with either population or hybrid rye grains. In conclusion, we found that feeding pigs with hybrid and population rye grains started adaptive changes in NEC. However, those changes were not profound, which allows us to speculate that adverse effects of these rye grains have a minor (if any) impact on the gut hormone balance (and indirectly on the health status) of animals.
11

Żywienie kur niosek paszą zawierającą żyto hybrydowe nie wpływa na właściwości ich ścięgien

42%
Muszynski S., Tomaszewska E., Swiatkiewicz S., Arczewska-Wlosek A., Dobrowolski P., Valverde Piedra J. L., Arciszewski M. B., Szymanczyk S., Zacharko-Siembida A., Kowalik S., Wesolowska-Trojanowska M., Kowal N., Schwarz T.
Pasze Przemysłowe
|
2019
|
tom 28
|
nr 1
12

Wpływ żyta hybrydowego w paszy niosek na wybrane cechy treści jaj

42%
Muszynski S., Tomaszewska E., Swiatkiewicz S., Arczewska-Wlosek A., Kasperek K., Dobrowolski P., Valverde Piedra J. L., Arciszewski M. B., Szymanczyk S., Zacharko-Siembida A., Kowalik S., Wesolowska-Trojanowska M., Schwarz T.
Pasze Przemysłowe
|
2019
|
tom 28
|
nr 1
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