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Phytopathogenic fungi and bacteria - causal agents of anthracnose, fusariose, grey mould, alternarial mould in lupine and bacterial blight in soya were isolated and identified. Microorganisms of genera Bacillus, Pseudomonas, Streptomyces with elevated antagonistic activity towards lupine and soya pathogens were selected. It was demonstrated that bacterial antagonists were able to suppress the development of phytopathogens both in vitro on agar nutrient media and on lupine/soya plants upon seed treatment. Selective action of antagonists against pathogenic species was established. Strains Pseudomonas aurantiaca S-l and Streptomyces anulatus M-46 were distinguished by the highest efficiency with respect to anthracnose and fusariose of lupine cultivars while bacteria Bacillus subtilis M-22 and Bacillus subtilis M-1 inhibited spread of lupine grey mould. Streptomycetes and bacilli displayed superior activity in control of soya bacterial blight. Based on the obtained findings biological method of lupine seed disinfection was elaborated and tested in field experiments. Completed investigations proved attractive prospects of biological method to control pathologies of leguminous crops as an ecologically safe alternative to chemical pesticides.
Highly active antagonistic actinomycète Streptomyces griseoviridis and entomopathogenic fungus Beauveria bassiana were applied to the soil separately and together (in association) in the laboratory experiments. We assessed survival rate, insecticidal and fungistatic activity of these strains. We also tested the influence of synthetic insecticide Regent 25® (fipronil 25g/l) on investigated parameters. Additionally, insecticidal activity of both strains was compared with insecticidal activity of Regent. It was shown that both strains, especially S. griseoviridis, good survived in soil. Population density of S. griseoviridis in 1he association with B. bassiana increased 2-3 times compared to initial density. Regent considerably reduced population density of S. griseoviridis and B. bassiana. Insecticidal efficiency of S. griseoviridis was comparable with the effect of synthetic incecticide Regent and reached 89.2% and 86.8% respectively. Fungistatic activity towards Fusarium oxysporum showed only S. griseoviridis and it was observed that this activity decreased in time course.
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