A pot experiment was carried out to study the effect of adenine applied at following rates: Q, 1,10 and 100 mg per 1 kg of typical brown soil on yield and chemical composition of carrot and on numbers of soil microorganisms as well as on activity of soil enzymes. Experiment was done in two series: with or without Azotobacter sp. inoculation. The favourable effect of applied adenine together with Azotobacter sp. on yield of carrot roots was observed. Adenine increased nitrogen and potassium uptake by carrot but adversely affected number of oligotrophic, macrotrophic, fixing of mineral nitrogen bacteria, ammonifers, Azotobacter sp. and actinomycetes. The inhibition action of this preparation on soil dehydrogenases, urease, and basic phosphatase and stimulation action on acid phosphatase have been found.
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