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Calcium (Ca²⁺) plays crucial roles in regulation of pollen tube growth. The influx of Ca²⁺ into the pollen tube is mediated by ion channels, and the density and activity of Ca²⁺ channels in pollen plasma membranes critically determines their electrical properties. In this report, using whole-cell and single-channel patch-clamping techniques, we investigated developmental changes of hyperpolarization-activated Ca²⁺ channel activity in pear (Pyrus pyrifolia) pollen and its relationship with pollen viability. For both pollen and pollen tubes, hyperpolarization-activated Ca²⁺ channels had the same conductance and cAMP sensitivity, indicating that they were the same channels. However, the Ca²⁺ current density in pollen tube protoplasts was greater than that in pollen protoplasts. Compared with day-3 flowers’ pollen, hyperpolarizationactivated Ca²⁺ current density was significantly lower in day 0 and day 3 flowers’ pollen, which was consistent with the pollen germination and pollen tube growth, indicating that pollen protoplasts’ increased Ca²⁺ current density may have enhanced the pollen viability. During pollen tube elongation, pollen tube plasma membrane Ca²⁺ current density increased with increased length pollen tubes up to 300 µm. All of these results indicated that hyperpolarization-activated Ca²⁺ channel activity was associated with in pear pollen development and may have a causal link between Ca²⁺ channel activity and pollen viability.
In order to improve the utilization of the steelmaking byproduct steel slag in the concrete industry, this paper mainly studied the cementitious and hydration properties of converter steel slag. X-ray diffraction (XRD), non-evaporable water amount, strength, and particle size distribution were measured and analyzed. The paste with steel slag and water can be slowly hardened, which indicates the weak cementitious capacity of steel slag. The hydration of steel slag is similar to that of cement. The hydration rate of steel slag is much slower than that of cement at the early age, while its rate is higher than cement at the curing age of 90 days. The hydration rate of steel slag increases as its specific surface area (SSA) increases, and the degree of hydration becomes similar in steel slag with varying SSA at the curing age of 180 days. The early-age hydration of cement can be inhibited to some extent when steel slag is added. And the inhibition effect increases as the replacement level of steel slag increases. There is a slow strength development of steel slag blend concrete with a low water-to-binder ratio at the early age, while this influence decreases as age increases. The mineral phases of steel-slag-blend hardened paste mainly includes hydration products (Ca(OH)₂ and C-S-H), inert components (C₂F, magnetite, and RO phase), and unhydrated phases (C₃S, C₂S, gehlenite, and C₁₂A₇).
Fly ash and ground iron ore tailings with fineness similar to cement were used as active and inert mineral admixtures in this study. This paper examines the compressive strength development of plain mortar and blend mortar containing fly ash and ground iron ore tailing. The mortar was cured at the same curing regime and a different water to cementitious material ratio was used. The autogenous shrinkage is also detected in the first seven days. At the early stage of hydration, fly ash and ground iron ore tailings played a role as physical filler during the hydration of the cementitious composites. As age increases, the pozzolanic reaction of fly ash became dramatic. Concrete with fly ash and ground iron ore tailings has a similar trend of autogenous shrinkage. As the amount of fly ash and ground iron ore tailings increases, autogenous shrinkage linearly decreases and their autogenous shrinkage is similar to each other. The autogenous shrinkage of concrete in one day is more than the total shrinkage of 80% in seven days.
The main aim of this study was to screen and identify cadmium-resistant fungus and to characterize its cadmium adsorption. A cadmium-resistant strain (CN35) was isolated from cadmium-polluted paddy soil. Based on morphlogical characteristics, internal transcribed spacers region and β-tubulin gene sequence phylogenesis analysis, the strain was preliminarily identified to be Penicillium sp. This strain was resistant to Cd at 45 mM with Cd adsorption rate up to 83.56%, and also resistant to other heavy metals such as Pb, Zn, and Cu. When Cd²⁺ concentration ranged from 2 to 5 mM, the fungal colony changed from yellow/green to red. The colony morphology was also affected by Cd²⁺ concentrations with protuberances forming on the colony surface at 20 mM. The strain CN35 was found to grow well at pH 4 to 8 at between 24ºC and 37ºC, and the optimal growth conditions were established to be at pH 4 and 30ºC. Fermented liquid of the strain is neither disease-causing nor inhibitory to rice seedling emergence, but rather improves rice seedling and root growth and enhances rice detoxification ability under Cd stress. Thus, the Cd-resistant fungus CN35 has the potential to treat Cd-polluted rice paddies.
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