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Carbon nanoparticles as transporters of melittin to glioma grade IV U87 cells in in vitro model

100%

Biniecka P., Jaworski S., Bugajska Z., Daniluk K.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2017

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tom 56[1]

Carbon nanoparticles as transporters of melittin to glioma cells in in vitro model.Substances derived from nature have natural cytotoxic properties, melittin, the main component of bee venom is one of them. It has the ability to destroy any lipid bilayer, therefore to be used in a cancer treatment it needs to be targeted. The aim is to create the drug delivery system, which would efficiently deliver the active substance to glioma cells. Carbon nanoparticles are considered to be a good agent in biomedical applications, due to their biocompatibility and small sizes. In this study five types of nanoparticles were used: pristine graphene (GN), nanographene oxide (nGO), graphite (G), nanodiamond (UDD) and hierarchical nanoporous carbons (HNCs) to target the melittin to cancer cells. The visualization of the drug delivery complexes of melittin and nanoparticles was done with transmission electron microscopy, the influence of the complexes on cell morphology and structure was pictured with scanning electron microscope. Moreover, in order to check the viability of the cells treated with melittin and the complexes of melittin and nanoparticles the PrestoBlueTM assay was done, also to specify the way of the cell death the annexin V/PI assay was carried out. The results indicate that various nanoparticles behave differently in a complex with melittin. The UDD, GN and nGO nanoparticles resulted in higher mortality than the melittin itself. Creating and applying such complexes of melittin with nanoparticles in glioma cancer treatment may be a promising solution in the therapy.

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Interaction of hierarchical nanoporous carbons (HNCs) with chicken embryo red blood cells (RBC)

88%

Bugajska Z., Jaworski S., Daniluk K., Biniecka P., Sosnowska M.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2017

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tom 56[1]

Interaction of hierarchical nanoporous carbons(HNCs) with chicken embryo red blood cells (RBC.The purpose of this study was to characterize toxicity of hierarchical nanoporous carbons (HNCs) on chicken embryo red blood cells (RBC). RBC are a perfect model to adapt the hemolysis assay in evaluation of the in vitro blood compatibility of nanoparticles. The samples of blood were treated with different concentration of HNCs (10 µg/ml, 50 µg/ml and 100 µg/ml). Hemolysis assay showed that the hemolytic activity depends on the dose of HNCs. The microscope observations have shown a difference in morphology between treated and untreated RBC: changes in cell membrane shape and the occurrence of pathological erythrocytes forms.

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Influence of melittin on viability and integrity of cell membrane on grade IV glioma

88%

Daniluk K., Jaworski S., Biniecka P., Bugajska Z., Sosnowska M.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2017

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tom 56[1]

Influence of melittin on viability and integrity of cell membrane on grade IV glioma. The grade IV glioma is one of the malignant human tumours. Today there is no effective treatment for this type of cancer. Alternative methods are sought-after in glioma treatment, and lately melittin has been found to be useful in anticancer therapy. The aim of the study was to investigate the effect of melittin on the viability and the integrity of cell membranes of the grade IV glioma cells. The U87 glioma line cells were treated of melittin in increasing concentrations (5, 10, 15, 20 and 50 µg/mL) and incubated for 24 hours. After incubation, the tests were performed in order to investigate the cell morphology, cell viability, membrane integrity and the way of cell death. The results have shown the devastating effect of melittin on the glioma cells. The melittin causes disintegration of cell membranes and induces cell death by apoptosis and less by necrosis.

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Green synthesis of silver nanoparticles by using aqueous mint (Mentha piperita) and cabbage (Brassica oleracea var. capitata) extracts and their antibacterial activity

76%

Sosnowska M., Kutwin M., Adamiak A., Gawin K., Bugajska Z., Daniluk K.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

|

2017

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tom 56[1]

Green synthesis of silver nanoparticles by using aqueous mint (Mentha piperita) and cabbage (Brassica oleracea var.capitata) extracts and their antibacterial activity. The objective of this study was the synthesis of silver nanoparticles (Ag-NP) using leaves of mint and cabbage extracts as the reducing and stabilising agents. The presence of nanoparticles was initially confirmed by the obtained colour and next by transmission electron microscope (TEM). TEM analysis of obtained Ag-NP indicated that their size ranged 5-50 nm for mint and 10-150 nm for cabbage. The antibacterial activity of nanoparticles against pathogenic strains Escherichia coli, Staphylococcus aureus and Salmonella enterica were assessed by evaluation of metabolic activity, using the PrestoBlue and XTT test. The higher inhibition of bacterial viability was observed against Gram negative (E. coli, S. enterica) than Gram positive (S. aureus) bacteria.

Ograniczanie wyników

4 Annals of Warsaw University of Life Sciences - SGGW. Animal Science

4 Bugajska Z.

4 Daniluk K.

3 Biniecka P.

3 Jaworski S.

3 Sosnowska M.

1 Adamiak A.

1 Gawin K.

1 Kutwin M.

4 2017

Carbon nanoparticles as transporters of melittin to glioma grade IV U87 cells in in vitro model

Interaction of hierarchical nanoporous carbons (HNCs) with chicken embryo red blood cells (RBC)

Influence of melittin on viability and integrity of cell membrane on grade IV glioma

Green synthesis of silver nanoparticles by using aqueous mint (Mentha piperita) and cabbage (Brassica oleracea var. capitata) extracts and their antibacterial activity