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Overexpression of odc (ornithine decarboxylase) in Datura innoxia enhances the yield of scopolamine

100%

Singh A., Nirala N. K., Das S., Narula A., Rajam M. V., Srivastava P. S.

Acta Physiologiae Plantarum

2011

tom 33

nr 6

Scopolamine is widely used for its anticholinergic properties. Because of higher physiological activity and less side effects the world demand of scopolamine is estimated to be ten times greater than other anticholinergic agents, hyoscyamine and atropine. Since natural production is limited, alternatives are required to boost the production. We report the introduction of mouse odc gene of polyamine biosynthesis pathway which is also the primary pathway of tropane alkaloids in Datura innoxia. Polyamines, mainly putrescine, serve as the common metabolite for tropane alkaloids and nicotine. We have overexpressed odc gene to modulate the metabolic flux downstream and eventually achieved higher accumulation of scopolamine in transgenic plants. Among six independent transformed lines one line (O10) produced scopolamine (0.258 µg/g dry weight) almost six times higher than that produced by control plants (0.042 µg/g DW). To our knowledge, this is the first report of odc overexpression in D. innoxia leading to higher scopolamine yield.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Das S.

1 Narula A.

1 Nirala N. K.

1 Rajam M. V.

1 Singh A.

1 Srivastava P. S.

1 2011

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Overexpression of odc (ornithine decarboxylase) in Datura innoxia enhances the yield of scopolamine