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Analysis of cytoplasmic variation in a cucumber germplasm collection using chloroplast microsatellite markers

100%

Hu J.-B., Li J.-W., Zhou X.-Y.

Acta Physiologiae Plantarum

2009

tom 31

nr 5

Nine PCR-based markers were developed from the microsatellites in non-coding regions of chloroplast genome of Cucumis sativus and used to detect chloroplast DNA variation. These markers successfully detected intraspecific polymorphism among 37 cucumber accessions containing Chinese native germplasms (CNGs) and non-Chinese germplasms (NCGs). Each marker detected between two and four alleles and the diversity value of the makers ranged from 0.105 to 0.528. Based on the data from allele size variation, a total of 17 distinct haplotypes were identified from the 35 accessions (excluding the two accessions possessing null genes). Three haplotypes were prevalent among CNGs but most NCGs had unique haplotype. No identical haplotype was found between CNGs and NCGs, reflecting lack of exchange of CNGs with others in the 60–80s of last century. A wild species (C. hystrix Chakr.) tested herein shared a haplotype with some CNGs, suggesting that it could be the ancestry of C. sativus or at least had a common ancestral lineage. The genetic relationship among the 37 cucumber accessions was further analyzed through construction of dendrogram based on Jaccard coefficient of similarity obtained from the allele sizes. All the CNGs were clustered into a group (containing the wild accession) that distinctly differed from the other four groups containing NCGs. This result agreed with the findings above obtained from haplotype analysis. Our research documented here will offer useful information for cucumber breeding.

Utilization of a set of high-polymorphism DAMD markers for genetic analysis of a cucumber germplasm collection

88%

Hu J.-B., Li J.-W., Wang L.-J., Liu L.-J., Si S.-W.

Acta Physiologiae Plantarum

2011

tom 33

nr 1

Eighteen minisatellite core sequences, derived from rice, human and phage M13, were used as primers in a PCR technique, known as directed amplification of minisatellite-region DNA (DAMD), to genotype 19 cucumber (Cucumis sativus L.) accessions from a wide collection. All the primers amplified polymorphic bands across the accessions. Out of 165 bands scored, 129 were polymorphic with 78.2% polymorphism. The average of polymorphism information content of the primers was 0.844, revealing a high discrimination power in cucumber. Based on Jaccard’s similarity indices and matrix generated by the DAMD markers, a dendrogram was constructed using the unweighted pair group method using arithmetic averages and allowed for separation of the 19 accessions into four distinct groups which demonstrated genetic relationship among the different types of germplasms. Sequencing of six polymorphic amplicons resulted in the identification of only one minisatellite locus, which indicated that variation in minisatellite number was not always the factor underlying DAMD polymorphism.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Hu J.-B.

2 Li J.-W.

1 Liu L.-J.

1 Si S.-W.

1 Wang L.-J.

1 Zhou X.-Y.

1 2011

1 2009

Wyniki wyszukiwania

Analysis of cytoplasmic variation in a cucumber germplasm collection using chloroplast microsatellite markers

Utilization of a set of high-polymorphism DAMD markers for genetic analysis of a cucumber germplasm collection