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Intensive environmental contamination by chemicals caused by human activities makes the monitoring of biological systems response necessary. It is difficult to predict the eff ects of pollutants basing only on measuring their concentrations in the abiotic environment. Toxic eff ects of pollutant are determined by its bioavailability for the accumulation in the body. Measurement of bioconcentration of pollutants in organisms is one of the types of biomonitoring. This allows the tracking the distribution of pollutants in the environment, not only allowing the assessment of environmental pollution, but also the real threat posed by these pollutants for the organisms living in this environment. Monitoring of bioconcentration of pollutant in conjunction with the analysis of their physiological effects and trophic relationships in ecosystems allows the prediction of ecological effects of pollution.
Seasonal changes in lipid droplet size and lipid peroxidation in the brown adipose tissue (BAT) of wild bank voles were examined. In addition, a role of photoperiod in these changes was studied; bank voles were held from the birth under long photoperiod (LP) for 12 weeks, and then half of them was transferred to short photoperiod (SP) for 6 weeks and another one remained under LP. In the wild bank voles the absolute BAT weight was seasonally constant, while the significant differences in the lipid droplet size were observed. The smallest lipid droplets (mean, 11 μm2) were seen in winter; they increased by 30 % in spring and reached the highest size (24 μm2) in summer. Lipid peroxidation in the BAT did not differ significantly between the seasons, although high intraseason variation of this process was noted. The laboratory experiment revealed that the size of lipid droplets was determined by photoperiod; SP induced 13-fold decrease, and continuous exposure to LP brought about a further 2.5-fold increase in the size of lipid droplets. Conversely, a significant decrease in lipid peroxidation was seen in LP bank voles in comparison with the SP animals. The data indicate that short photoperiod is responsible for the small size of lipid droplets in the BAT of bank voles during winter, which may be a necessary requirement for high thermogenic capacity of the tissue. Photoperiod appears also to affect lipid peroxidation in the BAT of these animals.
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