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Molecular identification of Buxtonella sulcata from associated-diarrhea in water buffaloes (Bubalus bubalis) in the Philippines

100%

Dianso J. A., Garcia G. G., Belotindos L. P., Mingala C. N.

Annals of Parasitology

2018

tom 64

nr 2

Sixty suspected protozoan oocysts were demonstrated from 260 fecal samples collected from water buffaloes aged one month to seven years old with clinical signs of diarrhea in four provinces in the Philippines after conventional methods of isolation, sporulation, morphological characteristics and Kinyoun Acid Fast Staining techniques. The recovered protozoan oocysts were subjected to molecular analysis. Amplification of DNA extracted from recovered Eimeria oocysts using universal primers for the ITS-1 region of 18S rRNA revealed PCR products with 348 bp size demonstrated by samples collected from Benguet, La Union and Nueva Ecija provinces in the Philippines while DNA extracted from oocysts of suspected Cryptosporidium spp. samples that applied primers for the SSU of 18S rRNA registered PCR products but no genes were amplified from diarrheic water buffaloes from these provinces. Alignment of the DNA sequences of the suspected Eimeria and Cryptosporidium species revealed sequences for three isolates of Buxtonella sulcata with product lengths that varied from 235 to 252 bp. This is an initial observation on the involvement of B. sulcata in diarrhea condition of water buffaloes in the Philippines. Phylogenetic analysis of the three local isolates of B. sulcata revealed no similarity with other protozoan constructed according to Neighbor-Joining method.

Molecular detection and characterization of Theileria species in the Philippines

100%

Belotindos L. P., Lazaro J. V., Villanueva M. A., Mingala C. N.

Acta Parasitologica

2014

tom 59

nr 3

Theileriosis is a tick-borne disease of domestic and wild animals that cause devastating economic loss in livestock in tropical and subtropical regions. Theileriosis is not yet documented in the Philippines as compared to babesiosis and anaplasmosis which are considered major tick-borne diseases that infect livestock in the country and contribute major losses to the livestock industry. The study was aimed to detect Theileria sp. at genus level in blood samples of cattle using polymerase chain reaction (PCR) assay. Specifically, it determined the phylogenetic relationship of Theileria species affecting cattle in the Philippines to other Theileria sp. registered in the GenBank. A total of 292 blood samples of cattle that were collected from various provinces were used. Theileria sp. was detected in 43/292 from the cattle blood samples using PCR assay targeting the major piroplasm surface protein (MPSP) gene. DNA sequence showed high similarity (90–99%) among the reported Theileria sp. isolates in the GenBank and the Philippine isolates of Theileria. Phylogenetic tree construction using nucleotide sequence classified the Philippine isolates of Theileria as benign. However, nucleotide polymorphism was observed in the new isolate based on nucleotide sequence alignment. It revealed that the new isolate can be a new species of Theileria.

Ograniczanie wyników

1 Acta Parasitologica

1 Annals of Parasitology

2 Belotindos L. P.

2 Mingala C. N.

1 Dianso J. A.

1 Garcia G. G.

1 Lazaro J. V.

1 Villanueva M. A.

1 2018

1 2014

Wyniki wyszukiwania

Molecular identification of Buxtonella sulcata from associated-diarrhea in water buffaloes (Bubalus bubalis) in the Philippines

Molecular detection and characterization of Theileria species in the Philippines