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We examined cadmium (0.1 mmol/l) toxicity and effect of different calcium concentrations (0.1, 1.0, and 10.0 mmol/l) on the growth of organs (root, mesocotyl and coleoptile) of 4-day-old maize seedlings in hydroponic cultures. The influence of both metals on distribution of cadmium, calcium, potassium and sodium in the organs of maize seedlings was also studied. Calcium at a concentration of 1.0 mmol/l increased the growth of all organs in comparison with the growth in 0.1 and 10.0 mmol/l CaCl₂. Cadmium inhibited maize organ growth in the presence of 0.1 and 1.0 mmol/l CaCl₂, whereas cadmium toxicity was not observed at 10.0 mmol/l CaCl₂. It was found that the content of both metals in maize seedling organs rose with increasing concentrations of Ca or Cd in the hydroponic solutions. Cadmium administration together with 10.0 mmol/l CaCl₂ led to an increase in calcium concentrations in roots and coleoptiles. The highest calcium concentration (10.0 mmol/l) caused a decrease in the cadmium concentration in roots. The addition of cadmium with 0.1 or 1.0 mmol/l CaCl₂ diminished potassium accumulation in the roots. By contrast, potassium content in the roots was not altered by cadmium when calcium was applied at a concentration of 10.0 mmol/l CaCl₂. Sodium content in maize seedlings was not affected in the presence of both cadmium and calcium at a concentration of 0.1 or 1.0 mmol/l, whereas cadmium with 10.0 mmol/l CaCl₂ decreased sodium accumulation in the roots. Thus, we conclude that the positive effect of highest concentration of calcium on the growth in the presence of Cd relay on the maintenance of high concentration of potassium in the roots.
Stwierdzono, że komórki zwierzęce i roślinne mogą pobierać kadm poprzez interakcje z wapniem. Wysokie koncentracje wapnia obniżały toksyczne działanie Cd²⁺ na różne procesy fizjologiczne. Kurtyka i in. wykazali, że Cd²⁺ hamuje indukowany auksyną (IAA) wzrost elongacyjny segmentów koleoptyli kukurydzy. Jednakże pozostaje niewyjaśnione do tej pory, czy Ca²⁺ może obniżać toksyczne działanie kadmu na indukowany IAA wzrost elongacyjny. W związku z powyższym wydało się celowym określenie zależności pomiędzy koncentracją kadmu i wapnia w środowisku i ich wpływu na indukowany przez IAA wzrost segmentów koleoptyli kukurydzy. Badania prowadzono na 10 mm segmentach koleoptyli wycinanych z 4- dniowych etiolowanych siewek kukurydzy. Wzrost segmentów koleoptyli kukurydzy mierzono zgodnie z metodyką opisaną wcześniej przez Karcza i in. Stosowano dwa stężenia jonów Ca²⁺: 0,1 mmol・dm⁻³ i 1 mmol・dm⁻³. Stwierdzono, że wapń w stężeniu 1 mmol・dm⁻³ obniża toksyczne działanie kadmu na wzrost elongacyjny segmentów koleoptyli kukurydzy. Podczas gdy wapń podany w tym samym stężeniu w obecności IAA nie znosi toksycznego działania Cd²⁺ na wzrost segmentów koleoptyli.
The effects of lead salts (chloride, nitrate, acetate) on the elongation growth of three cereal species (maize, wheat, rye) under the same growth conditions were studied. It has been found that the most toxic lead compound for maize was acetate, while the most sensitive to lead chloride was wheat. It is suggested that in order to obtain comparable results in experiments on the effects of different heavy metals on plant growth, the same salts of metals should be used.
In the present study the accumulation of Pb and Cd in maize seedlings (Zea mays L., cv. K33 x F2) and its effect on the content of Ca in their organs were investigated. The influence of both metals on dry weight yield was also studied. Concentrations of Pb, Cd and Ca were measured in 15 mm-long apical root segments as well as in whole mesocotyls and coleoptiles after 24 h of incubation of seedlings in hydroponic solutions. It was observed that in Pb- or Cd-treated maize seedlings the dry weight of root apical segments was markedly increased, whereas in the shoots it was less affected. It was also found that the content of both metals in maize seedling organs rose with increasing concentrations of Pb or Cd in the hydroponic solutions. The highest concentrations of both metals were observed in roots, in which the accumulation of Pb was 10-fold higher than that of Cd. By contrast, higher accumulation of Cd than Pb was found in mesocotyl and coleoptile. These data suggest that the mechanisms of Pb and Cd translocation from roots to shoots are different and that the accumulation of Ca in roots and its translocation to shoots was less affected by Pb than Cd.
This study investigates the effect of high concentrations of Pb and Zn on biomass production and accumulation of both metals in zinc violets (Viola calaminaria and Viola guestphalica) and two species of violets indigenous to Poland (Viola lutea sudetica and Viola tricolor). The influence of the plants on soil pH also was assessed. The soil used in the experiments, containing 183 mg kg-1 Zn and 53.4 mg kg-1 Pb, was spiked with Zn and Pb at 1,000 mg/kg soil (Treatment 1) or Zn at 10,000 mg/kg and Pb at 1,000 mg/kg (Treatment 2). Zn concentrations were found to be higher in roots than in shoots in all investigated species. In zinc violets, Zn content in the soil correlated with Zn concentrations in plant tissue, both roots and above-ground parts. There was no such dependence among indigenous violets. Since all confirmed hyperaccumulators accumulate metals preferentially in shoots, with lower concentrations in roots, our results suggest that zinc violets should not be classified as Zn hyperaccumulators. V. l. sudetica occurs naturally on soils with low heavy metals content. Its roots accumulated the highest amount of Zn (6,498 mg/kg) observed in the current study with no toxic effects. Roots were able to change the soil pH, but the differences were not significant. V. l. sudetica and V. tricolor increased biomass significantly in Treatment 1 versus the control, but in Treatment 2 neither species differed in biomass from the control. In Viola guestphalica no influence of treatments on dry weight was observed. We suggest that zinc violets can be useful for phytorestoration of contaminated sites.
This study presents root colonization of Deschampsia cespitosa growing in the immediate vicinity of a former Pb/Zn smelter by arbuscular mycorhizal fungi (AMF) and dark septated endophytes (DSE) at different soil depths. AMF spores and species distribution in soil profile were also assessed. Arbuscular mycorrhiza (AM) and DSE were found in D. cespitosa roots at all investigated soil levels. However, mycorrhizal colonization in topsoil was extremely low with sporadically occurring arbuscules. AM parameters: frequency of mycorrhization of root fragments (F%), intensity of root cortex colonization (M%), intensity of colonization within individual mycorrhizal roots (m%), and arbuscule abundance in the root system (A%) were markedly higher at 20–40, 40–60 cm soil levels and differed in a statistically significant manner from AM parameters from 0–10 and 10–20 cm layers. Mycorrhizal colonization was negatively correlated with bioavailable Cd, Pb and Zn concentrations. The number of AMF spores in topsoil was very low and increased with soil depth (20–40 and 40–60 cm). At the study area spores of three morphologically distinctive AMF species were found: Archaeospora trappei, Funneliformis mosseae and Scutellospora dipurpurescens. The fourth species Glomus tenue colonized roots of D. cespitosa and was observed in the root cortex at 20–40 and 40–60 soil depth, however, its spores were not found at the site.
Mineral forms in rhizosphere and bulk substrate were examined for selected plant species (Cardaminopsis arenosa, Calamagrostis epigeios, Deschampsia caespitosa, Festuca ovina, Silene vulgaris, Viola tricolor) growing spontaneously in the close vicinity of a tailings pond and in a zone of eolian transmission of waste particles. Samples of substrates and plant roots were taken from sites in the Bolesław orefield near Olkusz. The substrates together with plant roots were studied by scanning electron microscopy coupled with energy dispersive spectroscopy. Apart from quartz and carbonates, the most frequent mineral phases found were crystalline and amorphous Fe oxides, as well as primary Fe, Zn and Pb sulphides (marcasite, pyrite, sphalerite, galena). Hydrated Ca, Mg and Fe sulphates (gypsum, epsomite and melanterite) and other secondary minerals (smithsonite, cerussite, otavite, and Fe, K and Mg aluminosilicates) were found at larger concentrations in the rhizosphere than in bulk soil, suggesting that plant roots can change the mineral composition of the soil.
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