Wyniki wyszukiwania

1

Effect of different processing techniques on the nutritive value of grass pea, Lathyrus sativus L., seed meal in compound diets for Indian major carp rohu, Labeo rohita (Hamilton), fingerlings

100%

Ramachandran S., Ray A. K.

Archives of Polish Fisheries

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2008

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tom 16

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nr 2

Six isonitrogenous (35% crude protein approximately) and isocaloric (16.73 kJ g-1 approximately) diets incorporating processed grass pea, Lathyrus sativus L., seed meal at a 30% level by weight into a fish meal – based control diet were fed to rohu, Labeo rohita (Hamilton) fingerlings (average initial body weight 3.18 ± 0.11 g) in triplicate treatments at the rate of 3% of body weight daily for 80 days, and fish performance was studied. Four processing methods, namely fermentation, extrusion, autoclaving, and germination, were employed prior to the incorporation of the grass pea seed meal into the diets. The fermentation of grass pea seed meal was effective in significantly reducing the anti-nutritional factors, tannins, phytic acid, and the neurotoxin beta-ODAP (beta-oxalyl-diaminopropionic acid). The extrusion of grass pea seed was effective in significantly reducing tannins, trypsin inhibitor, and the neurotoxin beta-ODAP. Autoclaving the grass pea seed meal resulted in the reduction of tannins but was not effective in reducing other anti-nutritional factors. The level of trypsin inhibitor was reduced to non-detectable limits in germinated grass pea seeds. The tannin content was also reduced considerably in the germinated grass pea seed meal. In terms of growth response, feed conversion ratio, and protein efficiency ratio, 30% fermented, extruded, and germinated grass pea seed meal incorporated diets resulted in significantly (P < 0.05) the best performance of rohu fingerlings. The apparent protein digestibility (APD) values obtained with processed grass pea seed meal were significantly higher as compared to those with raw seed meal incorporated diets (P < 0.05). The accumulation of carcass protein was comparatively higher in the groups of fish reared on diets containing 30% autoclaved, germinated, and extruded grass pea seed meal. The results of this study indicate that processing grass pea seed meal is effective in improving the nutritive value of L. sativus seed meal and that the processed grass pea seed meal can be incorporated into rohu diets up to a 30% level without any adverse effect.

2

Improvement of quality of sal [Shorea robusta] seed meal protein with supplemental amino acids in feeds for rohu, Labeo rohita [Hamiltoan], fingerlings

100%

Mukhopadhyay N., Ray A. K.

Acta Ichthyologica et Piscatoria

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1999

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tom 29

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nr 1

25-39

An eight-week feeding trial was conducted in a static indoor rearing system to examine the effects of partial substitution of fish meal (FM) protein with deoiled salseed meal protein with and without supplemental amino acids in diets for rohu, Labeo rohita fingerlings (average weight 5.50 ± 0.19 g). Prior to incorporation into diets, deoiled sal (Shorea robusta) seed meal was fermented with lactic acid bacteria (Lactobacillus acidophilus) in order to reduce/eliminate the antinutritional factors, tannin and phytic acid present in it. Twelve experimental diets (diets D1 to D12) were formulated repalcing the FM protein from a reference diet with salseed meat protein at different levels (four sets of diets, of which each set ofthree diets contained 25%, 50%, and 75% replacement of FM protein by salseed meal protein respectively). Diets D1 to D3 were not supplemented with any amino acid. Lysine was supplemented to diets D4 to D6. Diets D7 to D9 were supplemented with methionine-cystine (together) and diets D10 to D12 contained lysine and methionine-cystine (together). Lysine and methionine-cystine (together) were added to the diets at 5.7% and 3.1% of dietary protein respectively. The groups of fish fed diets without any supplemental amino acids bad significantly lower percentage weight gain, SGR and higher FCR than the groups of fish fed other experimental diets. The addition of lysine and methionine-cystine to the diet in which 50% of FM protein was rep1aced by salseed meal protein (diet D11) significant1y improved fish weight gain and FCR. The percentage live weight gain and SGR values differed significantly (P < 0.01) from each other in the fish fed diets D10 to D12 which were supplemented with all three amino acids. The results of the present study suggest that rohu fingerlings can effectively uti1ise the supplemented amino acids and that deoiled salseed meal protein can replace up to 50% of FM protein in the diets for rohu if the salseed meal is properly processed (fermented) and supplemented with deficient amino acids.

3

Performance of rohu, Labeo rohita [Hamilton], fingerlings maintained on alternate feeding of plant and animal protein based diets

100%

Saha A. K., Ray A. K.

Acta Ichthyologica et Piscatoria

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1998

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tom 28

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nr 1

3-13

To understand the applicability of mixed feeding schedule in the fingerlings of the Indian major carp-rohu, Labeo rohita, an 80-day feeding experiment was conducted in the laboratory involving alternate feeding of plant and animal protein based diets with low and high levels of proteins, respectively. Feeding one day with soaked Leucaena (′subabul′/′ipil-ipil′) leaf meal based diet, followed by three days of animal protein (fish meal) based diet (feeding schedule 1S/3F) resulted in better performance of rohu in terms of live weight gain (%), SGR (%/day) and FCR as compared to those fed continuously with animal protein based diet (schedule F). Schedule 1S/3F also resulted in higher protein deposition in muscle and protein efficiency ratio. The results are expected to open up a new avenue to develop better feeding strategies with a view of reducing feed input cost by incorporating plant ingredients in the diets and by taking advantage of the existing natural variations in daily digestibility of protein.

4

Inclusion of extruded grass pea, Lathyrus sativus seed meal in compound diets for rohu, Labeo rohita [Hamilton, 1822] fingerlings

100%

Ramachandran S., Ray A. K.

Acta Ichthyologica et Piscatoria

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2004

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tom 34

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nr 2

Background. Grass pea, Lathyrus sativus is a widely available grain legume that contains a variety of anti-nutritional factors (ANFs). The aim of the present study was to determine the effects of feeding rohu, Labeo rohita, fingerlings with diets containing raw or extruded grass pea seed meal on growth, feed utilization efficiency, and carcass composition. Materials and Methods. Extrusion of finely ground grass pea seeds was performed in a twin-screw extruder at 130oC and 400 rpm. Six isonitrogenous (35% crude protein approximately) and isocaloric (16 547.7 J ּ g-1) diets were formulated incorporating raw and extruded grass pea seed meal at 20, 30, and 40% levels by weight into a fish meal based control diet. The diets were fed to rohu fingerlings in triplicate treatments at the rate of 3% of body weight for 60 days and fish performance in terms of growth, apparent protein digestibility (APD), and carcass composition was studied. Results. Extrusion of grass pea seed meal was effective in significantly reducing the ANFs, such as tannins (77%), trypsin inhibitor (below detection limit), and β-ODAP (46.09%). Phytic acid and nutrient components were not affected by extrusion. In terms of growth response, FCR and PER, 40% extruded grass pea meal incorporated diet resulted in significantly (P < 0.05) the best performance of rohu fingerlings. The APD values obtained for extruded seed meal incorporated diets were significantly higher in comparison to those for non-extruded ones. The carcass protein and lipid contents increased over the initial value in all dietary treatments with no significant differences in fish fed diets containing extruded grass pea seed meal. Conclusion. Extrusion is an effective treatment to reduce/eliminate the ANFs in legumes. Improved growth, feed utilization efficiencies, and APD in rohu fingerlings indicate that extrusion processing is also effective in improving the nutritional characteristics of grass pea.

5

Effect of fermentation on apparent total and and nutrient digestibility of linseed, Linum usitatissimum, meal in rohu, Labeo rohita, fingerlings

100%

Mukhopadhyay N., Ray A. K.

Acta Ichthyologica et Piscatoria

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2005

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tom 35

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nr 2

Background. Linseed and its by-products constitute a major source of dietary protein, but due to amino-acid imbalance and presence of anti-nutritional factors their use in fish feed is limited. Therefore, fermentation of linseed by lactic acid bacteria and/or fermented diets increases the level of linseed inclusion into the diet of rohu fingerlings by up to 40 percentage points. Materials and Methods. Six experimental diets (RL20, RL30, RL40, FL20, FL30, and FL40) for rohu fingerlings (Labeo rohita) were formulated using raw and fermented linseed meals. Finely powdered (400 µm) diet ingredients were incorporated at 3 different levels (20, 30, and 40%) into each diet, replacing equal proportions of all the ingredients from the reference diet. Two types of de-oiled linseed, Linum usitatissimum, meals were used as test ingredients: raw (RL) and fermented (FL). Prior to incorporation, a portion of the linseed meal was fermented by inoculating it with lactic acid bacteria, Lactobacillus acidophilus. The reference diet was prepared with fish meal as the chief protein source. Results. Fermentation of the oilseed meal resulted in reduction of tannin content from 2.45% to 1.32%. The total digestibility of the reference and test diets was significantly higher (P < 0.01) at all levels of fermented linseed addition. In particular, it was observed that the fermented samples showed comparatively better digestibility than the raw samples. Conclusion. Fermentation of oilseed meals which leads to the reduction of anti-nutritional factors may be applied as an efficient tool in formulation of feeds for rohu fingerlings.

6

Optimum dietary carbohydrate requirement of rohu, Labeo rohita [Hamilton], fingerlings

100%

Saha A. K., Ray A. K.

Acta Ichthyologica et Piscatoria

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2001

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tom 31

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nr 1

A laboratory feeding trial was conducted for 80 days using rohu, Labeo rohita, fingerlings (5.67 ±0.25 g) to evaluate the optimum dietary carbohydrate level. Five semipurified diets containing 30, 35, 40, 45, and 50% carbohydrate (diets D1 to D5) were formulated and fed to triplicate groups of fish. All the diets were isonitrogenous (35% protein) and almost isocaloric. Fish performance in terms of average live weight gain (%), SGR (%/day) and FCR was best with 40% carbohydrate level. Significantly (P < 0.05) poor growth of fish also was noticed in the groups reared on diets with 30 and 35% levels of dietary carbohydrate, however, no significant difference in PER and ANPU (%) was obtained with diets D2 and D3. Although no significant difference in apparent protein digestibility was recorded up to 45% dietary carbohydrate level, lipid, ash, dry matter, and energy digestibilities were found to be significantly (P < 0.05) higher for the diets D2 and D3. These two dietary treatments also resulted in higher deposition of protein and lipid and lower moisture and ash contents in the fish muscle. Amylolytic and proteolytic enzyme activities also were estimated to be higher in the groups of fish fed 35% and 40% carbohydrate diets. The study indicated that a minimum level of 40% dietary carbohydrate is required (protein content being 35%) for optimum growth, feed conversion and nutrient utilisation in rohu, Labeo rohita, fingerlings.

7

Optimization of fermentation conditions for cellulase production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut

81%

Ray A. K., Bairagi A., Sarkar Ghosh K., Sen S. K.

Acta Ichthyologica et Piscatoria

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2007

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tom 37

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nr 1

47-53

Background. Microbial and fungal cellulases are known to hydrolyse cellulose, which is ingested as plant material by herbivorous/omnivorous fishes. Microbial enzymes have enormous advantage of being produced in large quantities by established fermentation techniques. The present investigation aims to optimize the environmental and nutritional parameters for fermentation to enhance cellulase production by two bacterial strains isolated from fish gastrointestinal tracts. Materials and Methods. Two bacterial strains, Bacillus subtilis CY5 and Bacillus circulans TP3, isolated from the gastrointestinal tracts of common carp, Cyprinus carpio L., and Mozambique tilapia, Oreochromis mossambicus (Peters, 1852), respectively were identified as potent cellulase producers. Both strains were cultured in tryptone soya broth for 24 h at 32 ± 2°C, when average viable count of 9.75 • 107 cells • mL-1 culture broth was obtained. This was used as the inoculum for the production medium. The fermentation medium was seeded with 1.0%, 2.0%, 3.0%, 4.0%, and 5.0% inoculum (tryptone soya broth) and incubated in static culture at 40°C to standardize the inoculum size for fermentation. The effect of different production parameters, such as fermentation condition, moisture, pH, temperature, inoculum size, and nitrogen sources on cellulase production by the isolated bacterial strains were studied. Results. Cellulase yield was highest (26 U in B. subtilis and 20.2 U in B. circulans) in solid-state fermentation (SSF). Enzyme production in both the isolates increased in an optimum pH range of 7.0 to 7.5. Minimum cellulase production was observed at 45°C, while maximum production was obtained at 40°C. To standardize the fermentation period for cellulase production, production rate was measured at 12-h intervals up to 120 h. Enzyme production increased for 96 h of fermentation in both strains, and decreased thereafter. The enzyme production increased with increased inoculum size up to 3.0 percentage points. Asparagine as the nitrogen source was most effective in B. subtilis CY5, while beef extract proved useful in optimizing enzyme production by B. circulans TP3. Conclusion. The results of this study will help to standardize the requirements for optimum production of cellulase by cellulase-producing fish gut bacteria and might contribute towards better fish feed formulation incorporating plant ingredients, especially in the larval stages when the enzyme system is not efficient.

8

Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish

81%

Mondal S., Roy T., Sen S. K., Ray A. K.

Acta Ichthyologica et Piscatoria

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2008

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tom 38

|

nr 1

Background. The information on gut microflora in fish is scanty and there is a paucity of knowledge regarding microbial enzyme activity in fish gastrointestinal tracts. Although some information is available on the enzyme-producing bacteria in fish digestive tracts, almost nothing is known about their distribution in different regions of the gut. In the present study, an attempt has been made to investigate the distribution of enzyme-producing microflora in the foregut and hindgut regions of seven culturable freshwater teleosts. Materials and Methods. Isolation and enumeration of aerobic bacterial flora in the foregut and hindgut regions of the gastrointestinal tracts of seven freshwater teleosts of different feeding habits, namely rohu, Labeo rohita; catla, Catla catla; mrigal, Cirrhinus mrigala; bata, Labeo bata; orange-fin labeo, Labeo calbasu; Nile tilapia, Oreochromis niloticus; and climbing perch, Anabas testudineus, have been carried out. Microbial culture of the gut mucosa on selected nutrient media, following the enrichment culture technique, was done for bacterial isolation. Bacterial isolates were qualitatively screened on the basis of their extracellular enzyme-producing ability. The selected strains were further quantitatively assayed for amylase, cellulase and protease activities. Results. In general, bacterial population was lower in the foregut region of all the seven species of fish examined. Amylolytic strains were present in higher densities in the foregut region of orange-fin labeo and bata (12.20 × 103 CFU·g-1 gut tissue and 11.50 × 103 CFU·g-1 gut tissue, respectively) in comparison to the hindgut region. The cellulolytic population exhibited maximum densities in the hindgut region of bata (7.20 × 103 CFU·g-1 gut tissue) followed by the foregut region of the same fish (5.50 × 103 CFU·g-1 gut tissue). Amylolytic and cellulolytic bacterial flora was not detected in both the fore and hindgut regions of climbing perch. Proteolytic bacterial flora was found in all the species of fish studied and the maximum count was observed in the hindgut region of bata (13.40 × 103 CFU·g-1 gut tissue), orange-fin labeo (9.00 × 103 CFU·g-1 gut tissue), Nile tilapia (8.30 × 103 CFU·g-1 gut tissue) and climbing perch (7.20 × 103 CFU·g-1 gut tissue). Minimum count of proteolytic bacterial flora was observed in the foregut region of all the fishes studied. Peak amylase and cellulase activities were exhibited by bacterial strains isolated from the foregut of orange-fin labeo (266.43 ± 0.15 U) and the hindgut of bata (64.01 ± 0.42 U), respectively. Maximum protease activity was exhibited by a strain isolated from the hindgut region of orange-fin labeo (44.33 ± 0.09 U), followed by the strains isolated from the hindgut regions of climbing perch (32.87 ± 0.12 U), bata (29.71 ± 0.11 U), and Nile tilapia (29.46 ± 0.11 U). Conclusion. The results of the present study indicate that there is a distinct microbial source of digestive enzymes apart from the endogenous sources in fish digestive tracts. The enzyme-producing bacteria isolated from the digestive tracts can be beneficially used as a probiotic while formulating aquafeeds, especially in the larval stages. However, further investigations are required to determine if the addition of such isolates to fish feeds do, in fact, provide some kind of benefit to the fish involved before advocating their use.

9

Growth and survival of rohu, Labeo rohita [Hamilton, 1822] spawn fed diets fermented with intestinal bacterium, Bacillus circulans

80%

Ghosh K., Sen S. K., Ray A. K.

Acta Ichthyologica et Piscatoria

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2004

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tom 34

|

nr 2

Background. Because of the advancement of multiple spawning of carps, the importance of a quality larval diet is increasing day by day. The larval fish do not have the necessary enzyme or the amount of digestive enzymes to digest feed at optimum level. Therefore, fermentation of feed ingredients and/or formulated diets by bacterial enzymes to produce simpler forms of nutrients may be beneficial for them. Materials and Methods. Five isocaloric (4.38 kcalּg-1) and isonitrogenous (35% crude protein approximately) experimental diets (D1-D5) containing 32% fish meal, 34% mustard oil cake, 30% rice bran, and 2% cod liver oil, were fermented in vitro with Bacillus circulans cells (at the rate of 108 bacterial cells per g) at 37oC for 1-5 day duration (1 day: D1, 2 days: D2, 3 days: D3, 4 days: D4, and 5 days: D5). The bacterial strain [extracellular enzyme producer Bacillus circulans (Lr 1.1)] used for fermentation was isolated from the intestine of rohu, Labeo rohita fingerlings. The reference diet (RD), containing same ingredients, was not fermented with bacterial cells. Rohu spawn (av. wt. 0.35 Âą 0.01 mg) were fed ad libitum for 21 days in the laboratory condition at 30 minutes interval starting from 0900 h to 1600 h. Results. Fermentation of diets resulted in an increase in crude protein and free amino acid contents and decrease in crude fibre content. Diets D4 and D5 resulted in best growth and survival (98% and 98.33%, respectively) of rohu spawn compared to those fed with the reference diet (RD) and other experimental groups. A significant positive correlation was obtained between RNA : DNA ratio and specific growth rate (SGR) of 18 dietary groups (6 groups in triplicate). Conclusion. Fermentation of feed ingredients may be practiced as a tool for starter diet formulation to obtain better growth and survival of rohu spawn. The enzyme-producing strain, Bacillus circulans could be used to ferment diets for 4 to 5 days to increase the bioavailability of nutrients.

10

Effects of dietary protein and carbohydrate levels on growth performance, feed utilization efficiency and nitrogen metabolism in rohu, Labeo rohita [Hamilton], fingerlings

80%

Dalal S., Bhattacharya S., Ray A. K.

Acta Ichthyologica et Piscatoria

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2001

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tom 31

|

nr 2

A 60-day feeding experiment was conducted in the laboratory to evaluate the interactive effects of dietary protein and carbohydrate levels on growth, feed utilization efficiency, and nitrogen metabolism in rohu, Labeo rohita fingerlings (mean weight 4.06 ±0.08 g). Nine purified diets prepared with 25, 35, and 40 % protein level each having 15, 25, and 35 % carbohydrate were fed to nine different feeding groups at the rate of 3% of body weight in triplicate treatments. Better performance of fish in terms of percent weight gain, SGR, FCR, and PER was observed with increasing percentage of carbohydrate at a given protein level. Protease and α-amylase activities increased with increase in dietary protein and carbohydrate levels, respectively. Glutamate oxaloacetate transaminase (GOT) activities varied significantly in some groups. No significant difference among different groups regarding glutamate pyruvate transaminase (GPT) activity was observed. Ammonia excretion was found to increase with increased consumption of dietary protein and carbohydrate and was highest in the groups of fish fed 40% protein diet suggesting active nitrogen metabolism in these.

11

Growth and survival of rohu, Labeo rohita [Hamilton] spawn fed diets supplemented with fish intestinal microflora

80%

Ghosh K., Sen S. K., Ray A. K.

Acta Ichthyologica et Piscatoria

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2002

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tom 32

|

nr 1

A 21-day feeding experiment was conducted to assess the effects of diets supplemented with fish intestinal bacteria producing extracellular enzyme on growth and survival of rohu, Labeo rohita (Hamilton) spawn (average weight 0.3 mg). Intestinal bacteria were isolated from healthy rohu fingerlings (average weight 3.51 ±0.31 g). Six isocaloric (4.24 kcalּg-1) and isonitrogenous (approximately 35% crude protein) diets (D1-D6) were prepared containing 32% fish meal, 34% mustard oil cake, 30% rice bran, 2% cod liver oil, and 2% vitamin premix. Experimental diets (D2-D6) were supplemented with the isolated bacterial strain Bacillus circulans Lr 1.1 at five different levels (4 × 105, 4 × 106, 4 × 107, 4 × 108 and 4 × 1010 cells per 100 g of feed, respectively). Rearing of spawn on diet D5 (supplemented with 4 × 108 cells per 100 g) resulted in better growth and survival (97.33 ±1.15%) of rohu spawn compared to those with the control diet (diet D1, without any microflora supplementation) and other experimental diets. Diet D5 resulted in significantly higher growth and specific growth rate (SGR) followed by diets D6 and D4. RNA and DNA contents in the spawn were measured as an index of growth. The correlation coefficient (r value) of 0.831 and regression line having a slope of 0.79 suggested a significant positive correlation (0.01 level) between RNA-DNA ratio and specific growth rate.

12

Feed utilization efficiency and growth performance in rohu, Labeo rohita [Hamilton, 1822], fingerlings fed yeast extract powder supplemented diets

80%

Ghosh K., Sen S. K., Ray A. K.

Acta Ichthyologica et Piscatoria

|

2005

|

tom 35

|

nr 2

Background. Yeast extract powder (YEP) is a rich source of several B vitamins and is used mainly in bacterial culture. In the present study, the effect of dietary supplementation of YEP on growth, feed conversion, nutrient ADC, body composition, and digestive enzyme profile of rohu, Labeo rohita (Hamilton, 1822) fingerlings was evaluated. Materials and Methods. Rohu fingerlings (mean individual weight 1.76 ± 0.15 g) were fed, twice daily, fish meal-based 35% protein diets supplemented with YEP (0.1%, 0.2%, 0.3%, 0.4%, and 0.5% on dry matter basis) for 75 days at 3% of body weight, in triplicate. Fish performance in terms of growth, apparent nutrient digestibility, and whole body composition was studied. The intestinal protease and amylase activities were also determined in rohu fingerlings fed YEP-supplemented diets. Microbial cultures of the intestinal mucosa of the fish in all dietary treatments were carried out after the feeding trial in soybean-casein digest agar (TSA), gelatin-peptone (GP), starch (ST) and carboxymethylcellulose (CMC) plates, separately, to determine the protease- amylase-, and cellulase-producing capacities (qualitative) of the isolated organisms. Results. The fish fed YEP-supplemented diets exhibited better growth than the control up to 0.2% level. YEP at 0.1% level resulted in the best performance of the fish in terms of per cent weight gain, SGR, FCR, and PER, followed by the 0.2% level. YEP led to higher deposition of protein and lower deposition of lipid in the carcass. The intestinal protease activity was highest in the fish fed 0.1% yeast, followed by 0.2%. Protease-producing capacity of the intestinal bacterial flora in the in vitro cultures followed the same trend. Conclusion. It appears therefore that YEP in the diets might have some effect on the gut microflora by supplying additional digestive enzymes leading to better nutrient utilization.

13

Autochthonous gut bacteria in two Indian air-breathing fish, climbing perch (Anabas testudineus) and walking catfish (Clarias batrachus): mode of association, identification and enzyme producing ability

71%

Banerjee G., Dan S. K., Nandi A., Ghosh P., Ray A. K.

Polish Journal of Microbiology

|

2015

|

tom 64

|

nr 4

Scanning electron microscopy (SEM) was used to define the location of epithelium-associated bacteria in the gastrointestinal (GI) tract of two Indian air-breathing fish, the climbing perch (Anabas testudineus) and walking catfish (Clarias batrachus). The SEM examination revealed substantial numbers of rod shaped bacterial cells associated with the microvillus brush borders of enterocytes in proximal (PI) and distal regions (DI) of the GI tract of both the fish species. Ten (two each from the PI and DI of climbing perch and three each from the PI and DI of walking catfish) isolated bacterial strains were evaluated for extracellular protease, amylase and cellulase production quantitatively. All the bacterial strains exhibited high cellulolytic activity compared to amylolytic and proteolytic activites. Only two strains, CBH6 and CBH7, isolated from the DI of walking catfish exhibited high proteolytic activity. Maximum cellulase activity was exhibited by the strain, CBF2, isolated from the PI of climbing perch. Six most promising enzyme-producing adherent bacterial strains were identified by 16S rDNA gene sequence analysis. The strain ATH1 (isolated from climbing perch) showed high similarity to Bacillus amyloliquefaciens whereas, the remaining five strains (isolated from walking catfish) were most closely related to Bacillus licheniformis.

14

Comparative efficacy of phytase from fish gut bacteria and a commercially available phytase in improving the nutritive value of sesame oilseed meal in formulated diets for fingerlings of rohu, Labeo rohita (Actinopterygii: Cypriniformes: Cyprinidae)

61%

Roy T., Dan S. K., Banerjee G., Nandi A., Ghosh P., Ray A. K.

Acta Ichthyologica et Piscatoria

|

2016

|

tom 46

|

nr 1

Ograniczanie wyników

Effect of different processing techniques on the nutritive value of grass pea, Lathyrus sativus L., seed meal in compound diets for Indian major carp rohu, Labeo rohita (Hamilton), fingerlings

Improvement of quality of sal [Shorea robusta] seed meal protein with supplemental amino acids in feeds for rohu, Labeo rohita [Hamiltoan], fingerlings

Performance of rohu, Labeo rohita [Hamilton], fingerlings maintained on alternate feeding of plant and animal protein based diets

Inclusion of extruded grass pea, Lathyrus sativus seed meal in compound diets for rohu, Labeo rohita [Hamilton, 1822] fingerlings

Effect of fermentation on apparent total and and nutrient digestibility of linseed, Linum usitatissimum, meal in rohu, Labeo rohita, fingerlings

Optimum dietary carbohydrate requirement of rohu, Labeo rohita [Hamilton], fingerlings

Optimization of fermentation conditions for cellulase production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut

Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish

Growth and survival of rohu, Labeo rohita [Hamilton, 1822] spawn fed diets fermented with intestinal bacterium, Bacillus circulans

Effects of dietary protein and carbohydrate levels on growth performance, feed utilization efficiency and nitrogen metabolism in rohu, Labeo rohita [Hamilton], fingerlings

Growth and survival of rohu, Labeo rohita [Hamilton] spawn fed diets supplemented with fish intestinal microflora

Feed utilization efficiency and growth performance in rohu, Labeo rohita [Hamilton, 1822], fingerlings fed yeast extract powder supplemented diets

Autochthonous gut bacteria in two Indian air-breathing fish, climbing perch (Anabas testudineus) and walking catfish (Clarias batrachus): mode of association, identification and enzyme producing ability

Comparative efficacy of phytase from fish gut bacteria and a commercially available phytase in improving the nutritive value of sesame oilseed meal in formulated diets for fingerlings of rohu, Labeo rohita (Actinopterygii: Cypriniformes: Cyprinidae)