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The objective of the study was to ascertain the presence of complement binding antibodies against T. vaginalis, T. tenax and hominis in the blood of women with chronic simultaneous (two species) trichomoniasis, and a comparison of the dynamics of antibody formation in the blood of women with one species trichomoniasis. The determinations were made prior to and following the treatment with Fasigyn, in hope to obtain additional data on antigene properties of trichomonas parasites of human organism. The test for complement binding was made for 144 women, 84 of them simultaneously infected with two of the three species of Trichomonas mentioned above and 60 with one. It has been found that in the former the complement binding antibodies attain higher concentrations (except antibodies against T. hominis), and following the Fasigyn treatment in all the cases they return to the normal level later than in respective monospecific trichomoniasis. The highest concentrations of the antibodies, both against T. vaginalis and T. tenax have been found in women with simultaneous trichomoniasis of urogenital system and oral cavity. Lower concentrations of the antibodies against both these species of Trichomonas have been found in women with simultaneous trichomoniasis of both urogenital system and intestine or both intestine and oral cavity. This can be accounted for by a dose antigene relationship between T. vaginalis and T. tenax, and a much more distant relationship of both these species on the one hand and T. hominis on the other.
The authors studied immunological changes in 44 women with chronic trichomonadal colpitis and in 36 men with symptomless infection of urethra (age 19-50 years). In all the patients all other diseases were excluded. The presence of T. vaginalis in the vagina content and the secretion of the urethra was detected by means of microscope examination of fresh slides and of culture on Trimed medium. Besides, in all the patients complete morphological picture of blood as well as erythrocyte sedimentation rate (Biernacki reaction) were examined in order to find a relationship between these parameters. In the studies on the cellular immunological response the following tests were made: a) estimate of the number of early and late erythrocyte rosettes, and the absolute number of T lymphocytes in peripheral blood, by means of the test of rosette formation (it was assumed, after Bach, that a lymphocyte with five or more erythrocytes (of sheep) around it is a rosette), b) estimate of the absolute number of erythrocyte and B lymphocyte rosettes in peripheral blood, by means of the zymosan test of rosette formation; c) skin test of the delayed type with diluted tuberculine (1 : 5000), read after 24 hrs basing on the occurrence of erythema and induration. The results were statistically analysed using methods of comparative analysis. They were compared with the results of the tests on a control group comprising 40 healthy persons (20 men and 20 women) at age of 27-58 years. The results are presented in the table. The results of the test of erythrocyte sedimentation rate, not included in the table, were within the normal range in all the examined patients. Basing on the presented results the authors are of opinion that in the trichomoniasts immunological reactions of humoral and cellular type occur, both types being detectable almost to the same extent.
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