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Seed protein profiles were studied in 144 lentil accessions intensively collected from all over Pakistan. Heterogeneous populations were isolated on the basis of SDS-PAGE, and 13 polymorphic protein peptides were found, representing almost all the variation reported so far in lentil. The low diversity of accessions from the Northern Area and North Western Frontier Province, the most geographically diverse areas, suggested the need for more exploration so that the maximum genetic diversity of the areas can be truly represented. Clusters based on agro-ecological zones did not prove adequate for evaluation of lentil resources, whereas 63 of 108 accessions (58.3%) were grouped together by altitude and provincial distribution. The study confirmed the wealth of phenotypic divergence in the local lentil. A small sample of accessions from a particular region might not reflect the actual diversity within that region. Samples representing total diversity in particular countries or regions should be evaluated, so that a representative rather than a random set of accessions can be included in investigations of diversity on regional or continental scales. As Pakistan is in the vicinity of the centre of diversity of lentil, high variation of various parameters is expected, and that can be found only if a complete set of germplasm is studied.
Exposure to metals remains an important public health concern. The present study is a comprehensive field study to determine the concentrations of Cu, Zn, Ni, Cd, Co, Pb and Cr in water samples and in the livers of deceased freshwater turtles collected from pre-determined sampling sites of the Ravi and Chenab rivers. In total, 35 deceased freshwater turtles were collected from the sampling sites, and liver samples were collected and immediately stored at -20ºC prior to analysis. Heavy metals concentrations of Cu, Zn and Cd (0.073, 0.169 and 0.0042 mg/L⁻¹) in water samples were collected from the Ravi and (0.084, 0.145 and 0.0041 mg/L⁻¹) from the Chenab. Concentrations of Ni, Co, Pb and Cr were significantly lower (0.045, 0.018, 0.06 and 0.121 mg/L⁻¹) in the Ravi while (0.045, 0.018, 0.064 and 0.121 mg/L⁻¹) in the Chenab were significantly higher. Species-wise mean concentrations of all heavy metals were non-significant (P>0.05). The mean concentrations of Cu and Ni varied significantly (P<0.01) in the liver samples from the Ravi and Chenab. Regardless of the sampling sites, the mean concentrations of Cu and Pb recorded a maximum in K. Smithi (26.54±4.53 and 3.85±0.43 mg/Kg⁻¹, respectively) Zn in K. Tecta (42.48±4.06 mg/Kg⁻¹) and Ni, Cd and Co in L. Punctata. Among the studied metals, Zn was recorded as most abundant (39.73±2.28 mg Kg⁻¹) and Cd concentration was found to be minimum (0.188±0.017 mg Kg⁻¹) in freshwater turtles collected from both sampling sites.
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