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1

Surface active properties of bacterial strains isolated from petroleum hydrocarbon-bioremediated soil

100%
Plaza G. A., Ulfig K., Brigmon R. L.
Polish Journal of Microbiology
|
2005
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tom 54
|
nr 2
Two bacterial strains identified as Ralstonia picketti (BP 20) and Alcaligenes piechaudii (CZOR L-1B) were isolated from petroleum hydrocarbon-contaminated soil following bioremediation treatment. The surface active properties, e.g. surface tension, emulsification and foamability of their culture filtrates were evaluated. Bacterial cell-surface hydro-phobicity (BAH) as measured by analyzing cell affinity towards aliphatic and aromatic compounds was also determinated. The bacteria grew in liquid cultures containing 1% (v/v) of crude oil as carbon and energy source at 30°C under aerobic conditions. The surface tensions were reduced to 61 mN/m and 55 mN/m by Ralstonia picketti and Alcaligenes piechaudii, respectively. The emulsification index (EI24) was almost 100% for all tested compounds except diesel oil. The stability of the emulsions was determinated at 4°C, 45°C and 65°C. The emulsions were stable at 4°C. Ralstonia picketti was better foam inducer (FV = 50 ml) compared to Alcaligenes piechaudii (FV= 10 ml). The BAH measurements revealed higher adhesion of Alcaligenes piechaudii cells towards different hydrocarbons compared to Ralstonia picketti cells. The strains were found to have a surface hydrophobicity in the following order: aliphatic hydrocarbons, BTEX, and PAHs. The ability to adhere to bulk hydrocarbon is mostly a characteristic of hydrocarbon-degrading bacteria. The strains were found to be better emulsifiers than surface tension reducers. They produce water-soluble extracellular bioemulsifiers. Both bacterial isolates have good properties to use them, mainly in the petroleum industry, e.g. in enhanced oil recovery and in bioremediation processes-primarily due to their emulsification property, i.e. emulsion forming and stabilizing capacity.
2
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Study of antifungal activity of Bacilli species cultured on agro-industrial wastes

88%
Plaza G. A., Krol E., Pacwa-Plociniczak M., Piotrowska-Seget Z., Brigmon L. R.
Acta Scientiarum Polonorum. Hortorum Cultus
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2012
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tom 11
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nr 5
The three Bacillus species isolated from petroleum refinery waste were examined for antifungal activity on brewery effluents and molasses for biotechnological applications. Bacillus strains were identified by three different methods: 16S rRNA gene sequences, BIOLOG system and fatty acid analysis (FAME). The results demonstrated the ability of all three Bacillus strains cultured on brewery effluents and molasses to inhibit mycelial growth of the 10 tested fungi to varying degrees measured by agar plate inhibition assays. Fungi inhibited to the greatest degree as measured by the zones of inhibition were Botrytis cinerea A 258, Phomopsis viticola W 977, Septoria carvi K 2082, Colletotrichum gloeosporioides A 259, Phoma complanata A 233 and Phoma exigua var. exigua A 175. It was also observed that the fungal mycelial growth was inhibited by the cell-free supernatants, indicating lipoprotein-like activity of antifungal agents (mainly biosurfactants). Tested fungi were most sensitive to the Bacilli supernatants obtained from the molasses cultures including: B. cinerea A 258, R. solani W 70, S. sclerotiorum K 2291, Phomopsis diachenii K 657, C. dematium K 425, P. complanata A 233, P. exigua var. exigua A 175. In the previous study it was shown that Bacillus species produced biosurfactants. Application of natural products such as these Bacillus species or their byproducts may be a new approach to phytopathogen control therefore reducing the need for fungicides.
3

Rapid DNA extraction for screening soil filamentous fungi using PCR amplification

88%
Plaza G. A., Upchurch R., Brigmon R. L., Whitman W. B., Ulfig K.
Polish Journal of Environmental Studies
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2004
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tom 13
|
nr 3
A simple and rapid procedure for efficiently isolating fungi DNA suitable for use as a template for PCR amplification and other molecular assays is described. The main advantages of the method are: (1) the mycelium is directly recovered from Petri-dish cultures; (2) the technique is rapid and relatively easy to perform , and (3) it allows for processing of around 50 samples during a single day; (4) it is inexpensive; (5) the quality and quantity of DNA obtained are suitable for molecular assays; (6) it can be applied to filamentous fungi from soil as well as from a fungi from other environmental sources; and (7) it does not require the use of expensive and specialized equipment or hazardous reagents.
4

Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria

76%
Moryl M., Spetana M., Dziubek K., Paraszkiewicz K., Rozalska S., Plaza G. A., Rozaliski A.
Acta Biochimica Polonica
|
2015
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tom 62
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nr 4
5

Biodegradation of crude oil and distillation products by biosurfactant-producing bacteria

76%
Plaza G. A., Lukasik K., Wypych J., Nalecz-Jawecki G., Berry C., Brigmon R. L.
Polish Journal of Environmental Studies
|
2008
|
tom 17
|
nr 1
87-94
Biodegradation of crude oil and 7 different distillation products by Ralstonia picketti SRS and Alcaligenes piechaudii SRS was investigated. Crude oil degradation was above 80% after incubation of 20 days for both strains and their mixture. Removal of short/long alkanes and BTEX investigated was different, from 97% (for hexane degraded by the mixture of the isolates) to 22% (for tridecane degraded by Alcaligenes piechaudii SRS). Hexadecane, pristane and cyclohexane were degraded with high efficiency between 72-91%. Among BTEX, toluene was degraded very well by both strains and their mixture. Benzene and m+p-xylenes were efficiently degraded by the mixture of the isolates, 78% and 87%, respectively. Degradation of light boiling fractions achieved 80-100%. On the other hand, degradation removal of heavy, high boiling fractions was lower, and reached between 10-81%. The mixture of the isolates enhanced slightly the degradation efficiency of crude oil and its distillation products. Changes in crude oil toxicity as a function of biodegradation activity were also undertaken. Slight luminescence inhibition as a measure of toxicity was found during the experiment time. The samples were not found to be toxic to Vibrio fisheri during the crude oil biodegradation. The luminescence inhibition could be due to the presence of toxic intermediates produced during biodegradation.
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