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The objective of the present investigation was to evaluate the effect of nanodiamond (ND) particles on rats health status. 1 mg/kg b.w. of nanodiamond particles was administrated intravenously and intraperitoneum. The presence of an adverse impact was examined. The results show significant changes in biochemical (glucose and total protein level decrease) and hematological (elevated platelets count) parameters, only in case of intravenous injection.
This study was designed to examine the effect of high dose of L-ascorbic acid (L-AA) as a dietary supplement for rats on the phagocytic activity and oxidative burst of non-specific immune cells. Thirty Wistar rats (225-245 g initial body weight) were divided into three groups fed diet supplemented with 0.0, 0.3, and 0.6% L-AA (0, 187 and 375 mg L-AA/kg feed) for 41 days. At the end of the experiment, blood samples were analysed for selected indicators of non-specific immunity. The application of 0.6% of LAA, as compared to the remaining two groups reduced phagocytic activity measured as a number of opsonized E. coli cells consumed by neutrophiles and monocytes. However, 0.6% of dietary AA supplementation led to increased phagocytic monocytes and neutrophiles percentage,which produced reactive oxygen species after stimulation with opsonized bacteria (E. coli), phorbol 12-myristate 13-acetate (PMA) and the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP).These results indicate that megadose (0.6%) of dietary AA can reduce an oxygen-independent phagocytosis, but increase the number of neutrophiles and monocytes representing the oxygendependent mechanism of killing.
Effects of excretions/secretions produced by larvae of Lucilia sericata on growth of poultry isolates Salmonella Enteritidis in vitro. Salmonella Enteritidis is a major serovar associated with food borne salmonellosis in humans. The increased level of antimicrobial resistance observed in Salmonella has become a public health concern. There is an urgent need for a new ge­neration of antibiotics. The antibacterial properties of secretions collected from larvae of Lucilia sericata were examined in vitro. Studies revealed antibacterial activity of the larval excretion/secretion. The aim of this work was to define antibac­terial potentials of maggot ES for the treatment of salmonellosis. In order to evaluate this, ES were screened for antibiotic properties against 10 pathogenic S. Enteritidis strains. The antibacterial activities of ES were tested using the agar well diffusion method and colony forming unit (CFU) assay. Based on the obtained results we can conc­lude that: (a) ES demonstrate bacteriostatic effect against Salmonella ,(b) the highest antibacterial activity was revealed at 250 mg/L.
The objective of this experiment was to test the possibilities of measuring the rate of DNA synthesis in chicken embryos by applying a simple 15N tracer technique. We hypothesized that the rate of 15N incorporation into liver DNA depends on the type of labelled substance, reflecting precursor availability to provide substrates for nucleotide synthesis. Fertilized eggs were divided into 4 groups (4 × 15): control – not treated, and treated with 15N labeled glycine, ammonium chloride, or sodium nitrate. 15N labeled solutions were given in ovo by injection into albumen. After 20 days of incubation, the labeled substances had no effect on embryo development or morphology. Hepatic DNA was purified and 15N abundance was measured by isotope ratio mass spectrometry. There was significant enrichment of 15N in DNA from the glycine and ammonium chloride groups. We conclude that this simple technique of injecting 15N tracers into incubating eggs can be used to estimate the rate of DNA synthesis.
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