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Metal intake abilities of Euphorbia macroclada, Verbascum cheiranthifolium Boiss and Astragalus gummifer, which are common and native throughout Turkey and similar locations, were studied in the heavily polluted Keban mining district in Elazig, Turkey. For this aim metal contents of dried plants and soil were determined and correlated. Soils of Keban area have higher than average values for soil, Mo, Cu, Pb, Zn, Ag, As and Cd contents. All the studied plants take up metals in high amounts - as high as hundreds of times more than averages for non-hyperaccumulator plants. Usually, higher plant metal contents are attained where higher soil metal contents exist. Enrichment factors, which are calculated by dividing metal contents of plant by metal contents of soil (= metal content of plant/metal content of soil), are higher in lower soil metal contents. Maximum metal contents in the shoots (as mg kg⁻¹) and enrichment factors for Euphorbia are: Mo 260-1.28, Cu 33-0.18, Pb 76-0.09, Zn 190-0.51, Ag 0.53-1.1, Mn 276-0.28, As 10.2-0.08, and Cd 0.20-0.13. For Verbascum: Mo 80-0.83, Cu 27-2.87, Pb 295-1.57, Zn 254-1.78, Ag 0.37-0.92, Mn 627-0.58, As 63.5-0.50 and Cd 0.59-1.25. For Astragalus's gummufer: Mo 402-0.98, Cu 30-0.95, Pb 552-0.82, Zn 241- 0.31, Ag 0.54-0.64, Mn 1072-0.34, As 45.4-0.34 and Cd 0.34-0.44. All of the three plant species have enrichment factors exceeding hyperaccumulating criterion >1 for most of the elements investigated. Most of the hyperaccumulator values belong to Verbascum cheiranthifolium Boiss. Hyperaccumulating properties have been considered for reclamation of contaminated lands. This study claims that plants with high metal intake abilities escalate mobility of metals and increase contaminations on surface and subsurface.
Background: The purpose of this study was to compare different techniques for the estimation of spleen volume and surface area using magnetic resonance imaging (MRI) images, ultrasonography (USG) images and cadaveric specimen, and to evaluate errors associated with volume estimation techniques based on fluid displacement. Materials and methods: Five newborn cadavers, aged 39.7 ± 1.5 weeks, weighted 2.220 ± 1.056 g, were included in the present study. Three different methods were used to assess the spleen volume. The vertical section technique was applied using cycloid test probes for estimation of spleen surface area in MRI. Results: The mean ± standard deviation of spleen volumes by fluid displacement was 4.82 ± 3.85 cm³. Volumes determined by the Cavalieri’s principle using physical section and point-counting techniques were 4.45 ± 3.47 cm³ and 4.65 ± 3.75 cm³, respectively; volumes measured by USG and cadaver using ellipsoid formula were 4.70 ± 3.02 cm³ and 5.98 ± 4.58 cm³, respectively. No significant differences were found among all methods (p > 0.05). The spleen surface area was calculated as a 32.3 ± 20.6 cm² by physical sections using cadaver and also it was determined on axial, sagittal and coronal MR planes as 24.9 ± 15.2 cm², 18.5 ± 5.92 cm² and 24.3 ± 12.7 cm², respectively. Conclusions: As a result, MR images allow an easy, reliable and reproducible volume and surface area estimation of normal and abnormal spleen using Cavalieri’s principle. We consider that our study may serve as a reference for similar studies to be conducted in future. (Folia Morphol 2014; 73, 2: 183–192)
A 14-year-old male with a neck pain and hypoesthesia in the upper extremities was diagnosed with Chiari type I malformation (CMI) and syringomyelia. The posterior part of the occipital bone was removed via cranio-cervical decompression. The accuracy of measuring the posterior cranial fossa (PCF) and foramen magnum (FM) dimensions were evaluated and compared with the literature. The linear PCF and FM dimensions as well as volumes were measured using computed tomography (CT) images with different techniques. The volume data were compared with similar data from literature. Use of the posterior fossa approach remains controversial when treating patients with minor little brain stem dislocation, small PCF, and or incomplete C1, but the approach can easily be applied if FM and PCF sizes are known. Linear measurements that were assessed for concordance with CT measurements had the best agreement. Quantification of PCF volume and high FM should be taken into consideration for differential diagnosis of tonsillar herniation and prediction of surgical outcome in CMI. (Folia Morphol 2015; 74, 3: 402–406)
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