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1

Bezpieczeństwo i ochrona biologiczna na przykładzie Panstwowego Instytutu Weterynaryjnego - Państwowego Instytutu Badawczego w Puławach

100%
Skorupski A.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 11
The article describes the latest technical solutions enabling safe research on highly pathogenic biological material in the example of the National Veterinary Research Institute in Puławy. One of the priorities in conducting research on highly pathogenic microorganisms is to protect the environment from their spread. Many currently emerging diseases are zoonotic diseases, which can easily spread in the environment and are dangerous to both animals and humans. In order to eliminate the possibility of releasing pathogens from laboratories and experimental animal houses, a classification and technical requirements have been introduced to enable a safe conduct of research. There are four levels of biological safety, known as Biological Safety Levels (BSL). At level I, non-pathogenic microorganisms are used for research, which is conducted at open tables. Pathogens dealt with at level II are transmitted by blood and body fluids; infection may occur by contact with conjunctivas or through a breach in the skin surface. At this level, any contact of tested material with conjunctivas must be avoided, sharp objects must be secured, and sterile equipment must be used. Level III applies to airborne germs. Research is carried out in a laminar flow cabinet. To prevent transmission of microorganisms from the laboratory area, underpressure must be maintained with a proper ventilation system, and a number of control systems are required. Level IV applies to the most pathogenic microorganisms causing diseases of high mortality. Required protection measures include, among other things, a proper ventilation system, sluices with disinfectant showers and protective wear ensuring a complete isolation of the personnel from the environment and maintaining internal overpressure in relation to the laboratory environment. The above 4 levels of biological safety also apply to animals, and then the levels are known as ACL. This publication discusses the following systems: ventilation, electricity generation and maintenance, inactivation of contaminated sewage, communication between “clean” and “dirty” areas, waste disposal, decontamination of rooms and equipment, BMS surveillance and procedures.
2

Zastosowanie metody „suchej” i „mokrej” z wykorzystaniem gazowej formy nadtlenku wodoru w dekontaminacji pomieszczeń laboratoryjnych

51%
Skorupski A., Reichert M., Sikorski G.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 10
One of the latest and most effective disinfectants is the gas form of hydrogen peroxide (H₂O₂). The disinfection process runs in closed air circulation minimizing the threat to operating personnel. The advantage of the system is a broad spectrum of biocidal effects as well as the fact that the gas form of the biocidal substance has no impact on materials placed in the disinfected area (inter alia metals, plastics, installations, construction materials) and also guarantees the entire fill up of the gassed room. A comparison was made of “dry” and ”wet” disinfection methods of gas. For comparison purposes generators from Bioquell and Steris companies were chosen. Both devices are low-temperature mobile disinfection systems using a disinfecting agent in the form of 35% perhydrol and operating in a cubic capacity of up to 500 m³. In order to keep identical conditions during both processes, the comparison was made in the same room under similar physical conditions. Additionally, for the “wet” system process was carried out in a room of cubic capacity of 250 m³. To confirm the effectiveness of the decontamination processes microbiological tests using Geobacillus stearothermophilus bacteria (placed on metal discs and put in Tayvek bags) were applied. It was found that in both cases the decontamination systems demonstrated high biocidal efficiency. During the comparison of both methods it was noticed that in the case of the generator from Bioquell UK Company the optimization of the process intended to maintain a micro-condensation layer is possible only during decontamination of rooms of cubic capacities up to 250 m³. Above that cubic capacity the generator does not optimize the process. This means that the decontamination process conducted in such a manner is uncontrolled and may cause condensation of the disinfection agent on surfaces of expensive laboratory equipment. After the process some paint detachments were found in laboratories.
3

Miniaturowy nadajnik biotelemetryczny

51%
Kochman J., Przybylski A., Skorupski A.
Acta Physiologica Polonica
|
1970
|
tom 21
|
nr 3
4

Dekontaminacja pomieszczeń laboratoryjnych gazową formą nadtlenku wodoru

51%
Krajewska M., Skorupski A., Lipiec M.
Życie Weterynaryjne
|
2011
|
tom 86
|
nr 05
5
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Akarofobia - czy rodzice z Wielkopolski boją się kleszczy?

39%
Blasiak A., Opalinska P., Lukowski A., Krokowska-Paluszak M., Wierzbicka A., Skorupski A.
Studia i Materiały Centrum Edukacji Przyrodniczo-Leśnej
|
2017
|
tom 19
|
nr 1[50]
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