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1

Regulation of autoreactive B cells: checkpoints and activation

100%
Ding C., Yan Y.
Archivum Immunologiae et Therapiae Experimentalis
|
2007
|
tom 55
|
nr 2
2

Comparison of soil microbial biomass and enzyme activities among three alpine grassland types in Northern Tibet

81%
Lu X., Fan J., Yan Y., Wang X.
Polish Journal of Environmental Studies
|
2013
|
tom 22
|
nr 2
Soil microbial biomass and enzyme activities have an important influence on nutrient cycling. The temporal variation in soil microbial biomass C, N, and enzyme activities during the growing season were determined under three different alpine grasslands in Northern Tibet. The results showed that soil microbial biomass C, and N contents and enzyme activities of the alpine meadow (AM) and the alpine meadow steppe (AMS) sites were much higher than those of the alpine steppe (AS) site. Soil microbial biomass C, N variations were not significantly correlated with the soil temperature and moisture, except that microbial biomass N seemed associated with the microsite where soil temperature was higher. Our results demonstrate that soil temperature was one of most important factors explaining the seasonal variation of microbial biomass N, but how the alpine grassland ecosystem's type affects microbial biomass C, N and enzyme activity are still needed to be clarified by determining other correlative ecological factors and covering prolonged observation periods.
3

The association of brain derived neurotrophic factor gene variants with behaviour traits in sika deer (Cervus nippon)

81%
Lv S.-J., Yan Y., Wei W.-H.
Journal of Animal and Feed Sciences
|
2012
|
tom 21
|
nr 3
It is widely accepted that brain-derived neurotrophic factor (BDNF) is involved in modulating behaviour performance induced by environmental conditions. The aim of this study was to study polymorphisms of the BDNF gene and their relationship with animal behaviour in sika deer (Cervus nippon). Forty-eight sika deer reared at the Ping-Shan-Tang Farm (25 deer) and Zhu-Yu-Wan Park (23 deer), Yangzhou City, Jiangsu Province (China) were observed and blood samples were taken to identify BDNF genotypes. Data were subjected to ANOVA analysis to evaluate the link between genotype and animal behaviour traits. After PCR and electrophoresis, polymorphisms were found in two pairs of primers. At primer P-4, the AA genotype (26 deer) rested significantly less than the BB genotype (16 deer) (P<0.05). The AA genotype deer also exhibited significantly more locomotor behaviour (P=0.001). At primer P-5, deer of genotypes CC/DD/CD differed significantly in their watching behaviour. Deer of genotype CC exhibited significantly less resting and self-grooming behaviour than deer of genotypes CD or DD (both P<0.05). Our findings suggest that polymorphisms in BDNF may be involved in some aspects of animal behaviour traits, especially in the highly sensitive sika deer reared for several years in Chinese parks.
4

MiR-139-5p alleviates neural cell apoptosis induced by spinal cord injury through targeting TRAF3

81%
Zhang Z., Shen L., Yan Y.
Acta Biochimica Polonica
|
2020
|
tom 67
|
nr 3
5

Manipulation of cellular energy reveals the relationship between ultraweak luminescence and cellular energy during senescence of strawberry (Fragaria × ananassa) fruits

71%
Guo J., Liu H., Bai Y., Yan Y., Li L.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 07
6

Low-carbon development of the construction industry in China’s pilot provinces

71%
Du Q., Lu X., Yu M., Yan Y., Mu M.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 4
7

Leaf litter decomposition in three subalpine forests along an elevation gradient in Tibet

71%
Fan J., Zhang X., Lu X., Yan Y., Wang X.
Polish Journal of Environmental Studies
|
2014
|
tom 23
|
nr 4
Litter decomposition is a fundamental ecosystem process, and climate and litter chemistry strongly control rates of litter decay. In this work, three forests along an elevation gradient on the eastern slope of Sergyemla Mountain were selected to compare litter decomposition and chemical fraction loss rates, and further to evaluate the effects of environmental factors and litter chemistry on the litter decomposition process. The leaf litter decomposition coefficient of the mixed conifer and broadleaf forest (MCBF, 3,169 m a.s.l.), sclerophyllous evergreen broadleaf forest (SEBF, 3,453 m a.s.l.), and subalpine dark coniferous forest (SDCF, 3,957 m a.s.l.) sites were 0.04, 0.03, and 0.02 month-1, respectively. The litter mass loss at the MCBF site significantly correlated with litter quality, but that of the SEBF and the SDCF sites did not. In addition, there was a significant positive relationship between the litter mass loss and temperature along the elevation gradients. This study demonstrates that the litter decomposition rate decreases with increasing altitude along the elevation gradient. Climate is the key factor influencing litter decay across environmental gradients, but litter quality also affects decomposition rates in low-elevation forests.
8

Effects of salt stress and exogenous Ca2plus on Na plus compartmentalization, ion pump activities of tonoplast and plasma membrane in Nitraria tangutorum Bobr. leaves

71%
Liu W., Yuan X., Zhang Y., Xuan Y., Yan Y.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 08
Nitraria tangutorum Bobr. is a typical halophyte with superior tolerance to salinity. However, little is known about its physiological adaptation mechanisms to the salt environment. In the present study, N. tangutorum seedlings were treated with different concentrations of NaCl (100, 200, 300 and 400 mmol L⁻¹) combined with five levels of Ca²⁺ (0, 5, 10, 15 and 20 mmol L⁻¹) to investigate the effects of salt stress and exogenous Ca²⁺ on Na⁺ compartmentalization and ion pump activities of tonoplast and plasma membrane (PM) in leaves. Na⁺ and Ca²⁺ treatments increased the fresh weight and dry weight of N. tangutorum seedlings. The absorption of Na⁺ in roots, stems and leaves was substantially increased with the increases of NaCl concentration, and Na⁺ was mainly accumulated in leaves. Exogenous Ca²⁺ reduced Na⁺ accumulation in roots but promoted Na⁺ accumulation in leaves. The absorption and transportation of Ca²⁺ in N. tangutorum seedlings were inhibited under NaCl treatments. Exogenous Ca²⁺ promoted Ca²⁺ accumulation in the plant. Na⁺ contents in apoplast and symplast of leaves were also significantly increased, and symplast was the main part of Na⁺ intracellular compartmentalization. The tonoplast H⁺-ATPase and H⁺-PPase activities were significantly promoted under salt stress (NaCl concentrations ≤ 300 mmol L⁻¹). PM H⁺-ATPase activities gradually increased under salt stress (NaCl concentrations ≤ 200 mmol L⁻¹) followed by decreases with NaCl concentration increasing. The tonoplast H⁺-ATPase, H⁺- PPase and PM H⁺-ATPase activities increased first with the increasing exogenous Ca²⁺ concentration, reached the maximums at 15 mmol L⁻¹ Ca²⁺, and then decreased. The tonoplast and PM Ca²⁺-ATPase activities showed increasing trends with the increases of NaCl and Ca²⁺ concentration. These results suggested that certain concentrations of exogenous Ca²⁺ effectively enhanced ion pump activities of tonoplast and PM as well as promoted the intracellular Na⁺ compartmentalization to improve the salt tolerance of N. tangutorum.
9

Immune checkpoint blockade in cancer immunotherapy: mechanisms, clinical outcomes, and safety profiles of PD-1/PD-L1 inhibitors

71%
Yan Y., Zhang L., Zuo Y., Qian H., Liu C.
Archivum Immunologiae et Therapiae Experimentalis
|
2020
|
tom 68
|
nr 6
10
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Erratum to: Quantifying soil hydraulic properties and their uncertainties by modified GLUE method

71%
Yan Y., Liu J., Zhang J., Li X., Zhao Y.
International Agrophysics
|
2018
|
tom 32
|
nr 2
11

Effects of Na2CO3 and NaCl stresses on the antioxidant enzymes of chloroplasts and chlorophyll fluorescence parameters of leaves of Puccinellia tenuiflora (Turcz.) scribn.et Merr.

61%
Wang Y., Sun G., Suo B., Chen G., Wang J., Yan Y.
Acta Physiologiae Plantarum
|
2008
|
tom 30
|
nr 2
Antioxidant enzymes in chloroplasts and chlorophyll fluorescence parameters of leaves of Puccinellia tenuiflora (Turcz.) scribn.et Merr. under isotonic Na2CO3 and NaCl stresses were studied. Ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities showed a similar increasing trend and then decreased with the decreasing osmotic potential of culture solution, peaking at –4.74 x 10⁵ Pa under NaCl stress and at –3.40 x 10⁵ Pa under Na2CO3 stress. APX, glutathione transferase and SOD activities were higher under NaCl stress than those under Na2CO3 stress, and higher activities of antioxidant enzymes in chloroplasts were accompanied by lower MDA content under NaCl stress. Fv/Fm, Fv/Fo and Fv'/Fm' all initially increased and then decreased with the decreasing osmotic potential of culture solution, while ΦPSII, qNP and HDR showed a constant increase. Fv/Fm, Fv/Fo, ΦPSII and qNP under NaCl stress were also shown to be higher than those under Na2CO3 stress. The present study suggested that acidity played an important role in the hurt to Puccinellia tenuiflora seedlings, which was due to higher activities of antioxidant enzymes, qNP and ΦPSII, and the Na2CO3 resistance to Puccinellia tenuiflora was also supposed to be less than NaCl resistance in present work.
12

The future prospects of arbuscular mycorrhizal fungi in slope ecological restoration

61%
Yan Y., Zhao B., Xu W., Yu F., Liu W., Xia D.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 3
13

Initial start-up characteristics of anaerobic ammonium oxidation in a UASB reactor

61%
Cheng H., Yan Y., Yi X., Wang J., Xu W., Nie H.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 3
14

Analysis of recent coastline evolution due to marine reclamation projects in the Qinzhou Bay

61%
Yan Y., Zhang Z., Wang C., Zhang L., Huang Y., Zhang J.
Polish Maritime Research
|
2017
|
tom 24
|
nr Special Issue S2
Intense marine reclamation activities bring obvious benefits, meanwhile, these activities also influence the coastal natural formation, hydrodynamic processes and water environment etc. In order to investigate the exact influence in the Qinzhou Bay brought by marine reclamation behaviors in recent years, based on the remote sensing image data in the Qinzhou Bay in 2006, 2008, 2009, 2012 and 2014, with the help of GIS and RS software, the thesis interprets Qinzhou Bay coastline and artificial reclamation by man-machine interaction and makes quantitative analysis in the Qinzhou Bay coast evolution recently. The results show: (1) Qinzhou Bay coastal morphology change degree was strong in 2006 - 2012 and the coastline changed greatly. Originally part of the natural shore segments which had twists and turns were gradually filled into artificial coast, the coast constantly advanced to the sea, coastline became regular and straight; (2) The change degree of coastline was relatively small in 2006-2008, the average artificial coastline increased 8.86 km/a, the average artificial reclamation area was 20.79 km2 /a; in 2008-2012, the change range of coastline was the greatest, coastline changed most severely, the average artificial coastline increased 16.07 km/a, the average artificial reclamation area was 58.52 km2 /a; however, in 2012-2014, coastline change degree relatively slowed, the average artificial coastline increased 4.04 km per year, the average artificial reclamation area was 3.464 km2 /a. (3) Compared to the studies of predecessors, the innovation of this paper is that it is the first to carry out the detailed research on coastline evolution caused by reclamation engineerings of the Qinzhou Bay, then formed the change results on coastline that caused by intense human activities in the Qinzhou Bay, provided the area of the marine environment protection with full and accurate data
15

Gaba exist as a negative regulator of cell proliferation in spermaogonial stem cells

61%
Du Y., Du Z., Zheng H., Wang D., Li S., Yan Y., Li Y.
Cellular and Molecular Biology Letters
|
2013
|
tom 18
|
nr 2
γ-amino butyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA is also found in many peripheral tissues, where it has important functions during development. Here, we identified the existence of the GABA system in spermatogonial stem cells (SSCs) and found that GABA negatively regulates SSC proliferation. First, we demonstrated that GABA and its synthesizing enzymes were abundant in the testes 6 days postpartum (dpp), suggesting that GABA signaling regulates SSCs function in vivo. In order to directly examine the effect of GABA on SSC proliferation, we then established an in vitro culture system for long-term expansion of SSCs. We showed that GABAA receptor subunits, including α1, α5, β1, β2, β3 and γ3, the synthesizing enzyme GAD67, and the transporter GAT-1, are expressed in SSCs. Using phosphorylated histone H3 (pH3) staining, we demonstrated that GABA or the GABAAR-specific agonist muscimol reduced the proliferation of SSCs. This GABA regulation of SSC proliferation was shown to be independent of apoptosis using the TUNEL assay. These results suggest that GABA acts as a negative regulator of SSC proliferation to maintain the homeostasis of spermatogenesis in the testes
16

Effects of calcium Ionophore A23187 on the apoptosis of hepatic stellate cells stimulated by transforming growth factor-β1

61%
Li Y., Yan Y., Liu F., Wang M., Feng F., Xiao Y.
Cellular and Molecular Biology Letters
|
2018
|
tom 23
Background: Our previous study showed that during in vitro experiments changes in calcium concentration were associated with apoptosis. We presumed that the calcium ion might play a role as intermediate messenger for apoptosis-related genes. No such evidence has been reported in the literature. Here, we investigate the effect of calcium ionophore A23187 on the apoptosis of rat hepatic stellate cells (HSCs) stimulated by transforming growth factor-β1 (TGF-β1) to explore the mechanism of apoptosis through the endoplasmic reticulum stress pathway. Methods: The apoptotic rate was determined using flow cytometry. The changes in Ca2+ level in HSCs were examined with laser confocal microscopy. The expressions of caspase-12 GRP78 and caspase-9 were assayed via western blot. Results: The respective apoptosis rates for the blank group, the TGF-β1 group and the TGF-β1 + low, medium and high dose calcium ionophore A23187 groups were 3. 40 ± 0.10%, 1.76 ± 0.12%, 5.86 ± 0.31%, 11.20 ± 0.48% and 15.08 ± 0.75%, with significant differences between the groups (p < 0.05). The concentration of Ca2+ and the expression of the GRP78, caspase-9 and caspase-12 proteins significantly increased with increasing calcium ionophore A23187 doses (p < 0.05). Conclusion: Calcium ionophore A23187 increased intracellular Ca2+ and activated endoplasmic reticulum stress, which promoted HSC apoptosis.
17

Effect and mechanism of endophytic bacteria on growth and secondary metabolite synthesis in Salvia miltiorrhiza hairy roots

61%
Yan Y., Zhang S., Zhang J., Ma P., Duan J., Liang Z.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 05
Our study found that except Novosphingobium resinovorum (B5) Salvia miltiorrhiza root endophytic bacteria Pseudomonas brassicacearum sub sp. neoaurantiaca (B1), Rhizobium radiobacter (B2), Pseudomonas thivervalensis (B3), Pseudomonas frederiksbergensis (B4) significantly improved the activity of key enzymes 3-hydroxy-3-methyglutary1-CoA reductase and 1-deoxy-D-xylulose-5-phosphate synthase in the biosynthetic pathway of tanshinones. Specifically, HMGR activity with B1 treatment increased 2.1-fold that of control, 1-deoxy-Dxylulose-5-phosphate synthase activity with B2 treatment increased 5.0-fold that of control, which caused a significant increase in tanshinone content in the hairy roots. The dihydrotanshinone I and cryptotanshinone content under B1 treatment increased 19.2-fold and 11.3-fold, respectively, and total tanshinone content increased 3.7-fold that of control. The five endophytic bacteria B1, B2, B3, B4 and B5 all significantly decreased phenylalanine ammonialyase and tyrosine aminotransferase activity in hairy roots, of which, B3 treatment decreased phenylalanine ammonialyase activity by 46.2 %, and B2 treatment decreased tyrosine aminotransferase activity by 44.7 % compared with the control. Each of the five endophytic bacteria decomposed rosmarinic acid and salvianolic acid B, which caused a significant decrease in rosmarinic acid and salvianolic acid B content in hairy roots, with B2 treatment decreasing rosmarinic acid and salvianolic acid B content by 94.5 and 89.0 %, respectively, compared with the control. The five endophytic bacteria also inhibited the growth of S. miltiorrhiza hairy roots, of which, B2 and B4 treatment decreased hairy root biomass by 55.2 and 51.3 %, respectively, compared with the control, while hairy roots promoted the growth of B4 and B5 and inhibited the growth of B1 and B3.
18

Alterations in the proteome of wheat primary roots after wortmannin application during seed germination

61%
Cao H., Ni X., Zhang C., Shi W., Xu Y., Yan Y., Zhang F.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 10
19

Molecular characterization and evolutionary origins of farinin genes in Brachypodium distachyon L.

52%
Subburaj S., Luo N., Lu X., Li X., Cao H., Hu Y., Li J., Yan Y.
Journal of Applied Genetics
|
2016
|
tom 57
|
nr 3
20

The effects of ecological factors on the chemical compounds in Lycium barbarum L.

52%
Mi J., Yan Y., Li Y., Yin Y., Lu L., Luo Q., Li X., Zhou X., Niu Y., Cao Y.
Acta Physiologiae Plantarum
|
2020
|
tom 42
|
nr 05
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