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Natural zeolites contain micro- and macroelements such as calcium, magnesium, potassium, iron and copper. The aim of the following study has been to determine changes in poultry production induced by adding naturally occurring zeolites as dietary supplements. The study was carried out on 20,000 one-day chicks of the fattening breed called Ross. The birds were divided into two groups: the control group (group C) and the group supplemented with 5% of zeolite added to the feed (group E). Observations lasted for 45 days. Weekly measurements of the body weight of randomly chosen chicks were taken in both groups, and dead birds were counted. The composition of faeces was analyzed with an absorbance test. The air composition was determined with an instrument specially designed for that purpose. Differences in the body weight between groups C and E reached approximately 10%. A decrease in the mortality rate among chicks was observed in the treatment versus the control group. Mineral substances such as calcium and phosphorus were higher by about 4.4% and 9.5%, respectively, in the experimental group. Less ash in faeces (by about 20.4%) and less ammonia in the air (by about 33%) were determined for the experimental group, too. The present results may have important implications for poultry production, especially the fact that zeolite supplementation increased the body weight gain and reduced the number of falls. In conclusion, it is recommended to include zeolite in feed additives.
The aim of this study was to define the effects of diet containing the same mineral content of mineral salt or amino acid chelate, and diet containing various levels of Cu amino acid chelate on liver histomorphometry in growing rats. Male Wistar rats were used in the 12th week experiment. The control group (n = 12) was fed standard diet, which provided Cu in an inorganic form at the level required for rats. The experimental animals were divided into four groups (each n = 12) depending on different levels ( 100%, 75%, 50%, 25% covered daily demand) of Cu supplementation in chelated form. Cu content was determined in the liver tissue and blood plasma. Immunohistochemical staining with caspase-3 antibody was performed. Microscopic assessment of the liver structure indicated that Cu supplementation did not change the liver architecture. However, histomorphometric analysis revealed a significant increase in the number of nuclei, total cell number, and multinucleated hepatocytes in rats supplemented with the organic form of Cu at the level of 25% compared with the control group. There was a considerable increase in the number of apoptotic cells and ballooning degeneration of hepatocytes, especially in groups supplemented with organic form of Cu covering the daily demand in 100% and 75%, in comparison to control group. Moreover, there was no Cu deposition in the liver and changes in Cu content in blood. Cu provided in the diet in organic form covering an amount of its minimum daily demand in 25% appears to be the least harmful with regard to the liver. It indicates that there is a need to establish the level of diet supplementation with Cu amino acid chelates.
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