Wyniki wyszukiwania

1

Powtorzone sekwencje DNA typu REP i ERIC u bakterii - znaczenie diagnostyczne

100%

Ugorski M., Chmielewski R.

Postępy Higieny i Medycyny Doświadczalnej

|

2000

|

tom 54

|

nr 1

3-15

2

The macromolecular aggregate as a drug carrier

100%

Langer M., Ugorski M.

Cellular and Molecular Biology Letters

|

2000

|

tom 05

|

nr 4

433-440

Traditionally, a drug is expected to be biologically active and at the same time be able to ensure some sort of tissue or organ specificity. The latter property is necessary to avoid undesirable side effects when toxic drugs are being used. Such requirements are difficult to achieve only by changing the chemical formula of the drug. For these reasons, within the last few years a new pharmacological concept has been developed regarding delivery of biologically active compounds by the use of macromolecular aggregates. The purpose-specific design of macromolecular aggregates, able to deliver drugs to a desired location, is based on the assumption that different functions can be assigned to the separate chemical entities forming the aggregate. With the help of such an aggregate, the biologically active compound can be designed with solely its pharmacological potency in mind and without considering any limitations imposed by inaccurate delivery, such as undesired side effects. Specific molecules of the aggregate would ensure desired compound distribution within the organism. Furthermore, other molecules forming the aggregate should fulfill additional functions, e.g. protecting the drug from degradation. Additionally, aggregates formed from amphiphilic molecules should be capable of carrying drugs that are difficult to use as therapeutic agents due to low solubility in biological fluids (e.g. Taxol) or degradation (e.g. peptides, DNA). Such aggregates can be constructed from natural or/and synthetic compounds. Taken together, this creates possibilities of extending the spectrum of drug application and allows for the introduction of new technological modifications.

3

Alterations in glycoconjugates related to the transformation grade of human uroepithelial cell lines

100%

Ugorski M., Dus D.

Archivum Immunologiae et Therapiae Experimentalis

|

1995

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tom 43

|

nr 3-4

4

Sialyl Lewisa: a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells

100%

Ugorski M., Laskowska A.

Acta Biochimica Polonica

|

2002

|

tom 49

|

nr 2

Neoplastic transformation is often associated with characteristic changes in the expression of the sialyl Lewisa and sialyl Lewisx antigens, representing typical tumor-associated carbohydrate antigens. High amounts of sialyl Lewisa are present in human adenocarcinomas of the colon, pancreas and stomach. A growing amount of data suggests that this carbohydrate structure is the ligand for E-selectin. Sialylated Lewis structures present on the surface of tumor cells are carried by the carbohydrate chains of glycoproteins and glycolipids. There are several lines of evidence showing that sialyl Lewisa is responsible for the adhesion of human cancer cells to endothelium. E-selectin present on endothelial cells mediates these interactions. Selectins and their carbohydrate ligands can thus play an important role in the selective homing of tumor cells during metastasis. However, the presence of sialyl Lewisa antigen on the surface of tumor cells and their adhesion to E-selectin-expressing cells in in vitro adhesion assay by itself can not be directly related to metastatic properties of all cancer cells.

5

The role of cell adhesion in organ-specyfic metastasis

99%

Ugorski M.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr Supplement 1

6

Wplyw cytokin prozapalnych [TNF-alfa, IL-1 i IL-6] na czynnosc serca

81%

Paslawska U., Kiczak L., Ugorski M., Chelmonska-Soyta A.

Medycyna Weterynaryjna

|

2008

|

tom 64

|

nr 08

969-972

Cytokines are a group of peptides or small proteins that are involved in intercellular communication. Most of them are involved in local processes but some have endocrine activity. Cytokines are produced mainly by lymphocytes, monocytes, granulocytes, but also by fibroblasts, endothelial and epithelial cells. Disturbances of the integrity of tissues are the main inducers of cytokine synthesis connected with local immune response. On the other hand there is the group of constitutively produced cytokines that regulates the processes of hematopoesis, tissue remodeling, and lymphocytes migration. At present over one hundred biologically active substances described as cytokines have been identified. High levels of TNF-α, IL-1β i IL-6 are demonstrated both in severe cardiac insufficiency with cachexia and in the left sided cardiac failure. It has been established in several studies that proinflamatory cytokines contribute to dilated cardiomiopathy involvement, decreased blood perfusion in skeletal muscles, and exert endothelial injury of vessels, develop cachexia and inappetance and stimulate cardiac myocytes apoptosis. It was confirmed that cardiac myocytes are able to synthetize TNF-α in response to different types of overload, e.g. volume overload, pressure or ischemic (post infarct). TNF-α and IL-2 impart a negative inotropic effect on the heart proportional to their concentration. TNF-α, IL-1 and IL-6 regulate cardiac functioning indirectly due to the activity of the following: nitric oxide, reactive oxygen species, sphingolipid mediators, arachidonic acid and the modulation of β-adrenergic response.

7

Rola fimbrii typu 1 w patogenezie zakażeń pałeczkami Salmonella

81%

Ugorski M., Kuzminska-Bajor M., Kisiela D.

Postępy Mikrobiologii

|

2011

|

tom 50

|

nr 1

8

Isolation of Yersinia enterocolitica from aborted fetuses and sows in pig farms with reproductive disturbances

81%

Platt-Samoraj A., Szweda W., Ugorski M.

Polish Journal of Veterinary Sciences

|

2009

|

tom 12

|

nr 2

189-193

The purpose of the study was to determine the occurrence of Yersinia enterocolitica in tissues of aborted fetuses, placentas, vaginal and rectal swabs of aborting sows from pig farms where reproductive disturbances were found and to determine and analyze the biotype and serotype affinity of the strains isolated. Altogether 97 fetuses aborted in various stages of pregnancy, 25 placentas and swabs from 231 sows were taken. All sows originated from farms where reproductive disorders appeared. In general, 1069 samples were collected. Two enrichment methods were used in this study; fast enrichment techniques in ITC broth, then plating onto CIN agar (ITC/CIN), and cold enrichment in phosphate buffered saline followed by plating onto CIN agar (PBS/CIN). From all samples examined, 96 Y. enterocolitica strains were isolated including 57 (59.4%) from rectal swabs of sows, followed by 6 (6.3%) from vaginal swabs and 2 (2.1%) from placentas. The bacteria were isolated from tissues of 18 out of 97 aborted fetuses. A total of 60 strains were selected for further examination - 29 strains originated from aborting sows and 31 from aborted fetuses. Among strains examined 54 isolates (90%) belonged to the biotype 1A of Y. enterocolitica and to the different serotypes 0:3, 0:5, 0:6, 0:7/13, 0:8 and NT (not typable). Only 6 strains belonged to serotype 0:3, biotype 4 Y. enterocolitica. Our study has revealed the possibility of Y. enterocolitica isolation from internal organs of aborted swine fetuses and sows from farms with reproductive disturbances. The results suggest the connection between fetal death, pregnancy course disorders and Y. enterocolitica infection.

9

Wykrywanie zakazen wirusem zapalenia tetnic koni w nasieniu ogierow metoda cytometrii przeplywowej

81%

Cierpisz J., Golnik W., Laskowska A., Ugorski M.

Medycyna Weterynaryjna

|

1998

|

tom 54

|

nr 09

626-627

In the study flow cytometry was attempted to identify EAV infection in stallions’ semen. Material for the study consisted of 8 semen samples taken from EAV-seropositive stallions. The samples were put on RK-13 and Vero cells. After a 24-hour incubation period the cells were treated with a specific conjugate and then analyzed in FACS aparatus. In the case of two samples positive fluorescence was observed. The above positive result was confirmed by virus isolation in both cell cultures used.

10

Fimbrie Salmonella enterica serowar Enteritidis

81%

Ugorski M., Kisiela D., Wieliczko A.

Medycyna Weterynaryjna

|

2001

|

tom 57

|

nr 10

714-718

11

Ceramide galactosyltransferase (UGT8) as a molecular marker of canine mammary tumor malignancy

81%

Nowak M., Dziegiel P., Madej J., Ugorski M.

Folia Histochemica et Cytobiologica

|

2013

|

tom 51

|

nr 2

12

Genotype analysis of Campylobacter jejuni isolated from broilers in Poland

81%

Wojciech L., Bednarski M., Wieliczko A., Ugorski M.

Polish Journal of Veterinary Sciences

|

2005

|

tom 08

|

nr 1

13

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Wystepowanie i obraz patomorfologiczny gasterofilozy koni z polnocno-wschodniej Polski. Badanie morfologiczne oraz roznicowanie gatunkow larw z rodzaju Gasterophilus w oparciu o sekwencje nukleotydowa genu CO1

81%

Pawlas-Opiela M., Lukasz W., Ugorski M., Soltysiak Z.

Wiadomości Parazytologiczne. Suplement

|

2007

|

tom 53

56

14

Plasmid condensation induced by cationic compounds: hydrophilic polylysine and amphiphilic cationic lipid

81%

Chaszczewska-Markowska M., Ugorski M., Langner M.

Cellular and Molecular Biology Letters

|

2004

|

tom 09

|

nr 1

The construction of an efficient carrier for genetic material is a major research objective that needs to be achieved before gene therapy can become a viable pharmacological approach. Artificial aggregates containing nucleic acids are one of the options for the systemic delivery of genetic information. The diversity of functions the aggregate is expected to fulfill necessitates its complex architecture. In order to obtain a complex supramolecular aggregate, formed from elements that are themselves complex molecules, appropriate procedures based on the detailed understanding of processes at the molecular level are required. In this study, we investigated how the various properties of cationic compounds affect nucleic acid condensation. The combination of two condensing agents, differing in their affinity towards water, when mixed with plasmids, resulted in aggregates which are resistant to enzymatic digestion and which form particles with well-defined size distributions. Such uniform and well-defined complexes may subsequently be further modified in order to obtain a fully functional genetic material carrier.

15

Peanut agglutinin [PNA] binding glycoproteins on human urothelial cell lines of different grades of transformation

81%

Dus D., Ugorski M., Gorczyca W., Radzikowski C.

Archivum Immunologiae et Therapiae Experimentalis

|

1995

|

tom 43

|

nr 5-6

16

Analiza genotypowa paleczek Salmonella Gallinarum i Salmonella Pullorum wyizolowanych od niosek

71%

Chmielewski R., Wieliczko A., Kuczkowski M., Mazurkiewicz M., Ugorski M.

Medycyna Weterynaryjna

|

2001

|

tom 57

|

nr 10

731-735

17

Oznaczanie przeciwciał antymikrosomalnych i przeciwciał skierowanych przeciwko peroksydazie tarczycowej w autoimmunologicznych chorobach tarczycy

71%

Nestorowicz M., Steciwko A., Noczynska A., Sypniewska G., Ugorski M.

Diagnostyka Laboratoryjna i Wiadomości PTDL

|

1996

|

tom 32

|

nr 2

18

Wlasciwosci fenotypowe paleczek Salmonella Gallinarum i Salmonella Pullorum wyizolowanych od kur niosek

71%

Wieliczko A., Kuczkowski M., Chmielewski R., Mazurkiewicz M., Ugorski M.

Medycyna Weterynaryjna

|

2001

|

tom 57

|

nr 09

671-675

19

CEA-negative glioblastoma and melanoma cells are sensitive to cytosine deaminase-5-fluorocytosine therapy directed by the carcinoembryonic antigen promoter

71%

Dabrowska A., Szary J., Kowalczuk M., Szala S., Ugorski M.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 3

Recent studies have suggested that carcinoembryonic antigen (CEA)-promoter sequences are active only in CEA-positive cells, filing in the criteria for tumor specific targeting of suicide genes. However, the present study on gene therapy of colon cancer and cell-specificity of CEA promoter, provide evidence that CEA-positive and CEA-negative cells transfected with E. coli cytosine deaminase (CD) gene under the control of CEA promotor sequence are sensitive to enzyme/pro-drug therapy with 5-fluorocytosine (5-FC). Individual clones derived from the CEA-negative cell lines: melanoma Hs294T and glioblastoma T98G after transfection with CD differed profoundly in their sensitivity to 5-FC. The IC50 values for several clones of the CEA-neg- ative cells were almost the same as for CEA-positive colon cancer cells. Such 5-FC-sensitive clones derived from the population of CEA-negative cells, present even in small number, because of the very effective bystender effect of this enzyme/pro-drug system can cause severe problems during therapy by efficiently killing surrounding normal cells. Safety is the major issue in gene therapy. Our data suggest that the safety of gene-directed enzyme pro-drug therapy (GDEPT) with CEA promoter driven expression of therapeutic genes is not so obvious as it has originally been claimed.

20

Liposomes loaded with 5-fluorocytosine and their possible application to suicide gene therapy of cancer cells modified with the bacterial cytosine deaminase gene

71%

Chaszczewska-Markowska M., Stebelska K., Grzybek M., Sikorski A. F., Ugorski M.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

Ograniczanie wyników

Powtorzone sekwencje DNA typu REP i ERIC u bakterii - znaczenie diagnostyczne

The macromolecular aggregate as a drug carrier

Alterations in glycoconjugates related to the transformation grade of human uroepithelial cell lines

Sialyl Lewisa: a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells

The role of cell adhesion in organ-specyfic metastasis

Wplyw cytokin prozapalnych [TNF-alfa, IL-1 i IL-6] na czynnosc serca

Rola fimbrii typu 1 w patogenezie zakażeń pałeczkami Salmonella

Isolation of Yersinia enterocolitica from aborted fetuses and sows in pig farms with reproductive disturbances

Wykrywanie zakazen wirusem zapalenia tetnic koni w nasieniu ogierow metoda cytometrii przeplywowej

Fimbrie Salmonella enterica serowar Enteritidis

Ceramide galactosyltransferase (UGT8) as a molecular marker of canine mammary tumor malignancy

Genotype analysis of Campylobacter jejuni isolated from broilers in Poland

Wystepowanie i obraz patomorfologiczny gasterofilozy koni z polnocno-wschodniej Polski. Badanie morfologiczne oraz roznicowanie gatunkow larw z rodzaju Gasterophilus w oparciu o sekwencje nukleotydowa genu CO1

Plasmid condensation induced by cationic compounds: hydrophilic polylysine and amphiphilic cationic lipid

Peanut agglutinin [PNA] binding glycoproteins on human urothelial cell lines of different grades of transformation

Analiza genotypowa paleczek Salmonella Gallinarum i Salmonella Pullorum wyizolowanych od niosek

Oznaczanie przeciwciał antymikrosomalnych i przeciwciał skierowanych przeciwko peroksydazie tarczycowej w autoimmunologicznych chorobach tarczycy

Wlasciwosci fenotypowe paleczek Salmonella Gallinarum i Salmonella Pullorum wyizolowanych od kur niosek

CEA-negative glioblastoma and melanoma cells are sensitive to cytosine deaminase-5-fluorocytosine therapy directed by the carcinoembryonic antigen promoter

Liposomes loaded with 5-fluorocytosine and their possible application to suicide gene therapy of cancer cells modified with the bacterial cytosine deaminase gene