Usefulness and efficiency of different in vitro techniques (micropropagation, distant hybridization with using embryo rescue techniques and desired trait screening) in fruit plant breeding program was shown, basing on the results of studies realized in Department of Plant Genetics and Biotechnology of Horticulture Institute in Kaunas. Under the optimized conditions, the efficiency of plum micropropagation was 3-6 shoots per explant. In micropropagation of Japanese quince the highest rooting average (about 43%) was obtained at a medium pH lower than 5.0. The most important factor of embryo rescue efficiency was the time of isolation (43rd day after pollination). Results of in vitro screening of desired plant characters were confirmed by in vivo analysis at the level from 20 to 100%, depended on poly- or monogenic type of trait.