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Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

100%
Zabolewicz T., Barcewicz M., Brym P., Puckowska P., Kaminski S.
Polish Journal of Veterinary Sciences
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2014
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tom 17
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nr 4
This study analyzed the association between single nucleotide polymorphism (A/C) in position -28 located in the TATA box of LTF gene and the lactoferrin concentration in bovine milk secreted by healthy and infected udders. Out of 241, 69 cows were selected into the experimental group and were divided into 3 groups according to mean value of somatic cell count (SCC): I <180 000 cells/mL, II: 180 000 - 350 000 cells/mL and III > 350 000 cells/mL. In each SCC group, three LTF genotypes: AA, AC and CC were identified by PCR-SSCP method. A total of 604 milk samples were collected monthly and lactoferrin concentration was measured by ELISA. The 1-way ANOVA within SCC groups was performed to estimate association of -28 A/C genotypes with mean lactoferrin concentration per lactation. In the group of healthy cows (<180 000 cells/mL) LTF concentration in milk cows with the AA genotype (107.58 ± 17.92 μg/mL) was significantly higher than in homozygotes CC (52.09 ± 19.01 μg/mL). Unexpectedly, in cows with elevated SCC (>350 000 cells/mL) we observed a significant opposite relationship (207.21 ± 28.50 in CC vs 115.0 ± 28.6 μg/mL in AA). We hypothesized that a promoter with allele C, which cannot be recognized as a TATA sequence is becoming more accessible for other transcription factors, which may induce alternative LTF gene expression. We assume that our results demonstrate a very interesting effect of differential gene expression depending on polymorphism in a key regulatory motif (TATA box) and also on the health status of mammary tissues.
2
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Screening of Polish Holstein-Friesian bulls towards eradication of Complex Vertebral Malformation (CVM) carriers

100%
Rusc A., Hering D., Puckowska P., Barcewicz M., Kaminski S.
Polish Journal of Veterinary Sciences
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2013
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tom 16
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nr 3
The effectiveness of a program aimed at eradicating carriers of the recessive disorder Complex Vertebral Malformation (CVM) from the population of Holstein-Friesian bulls is reported. Among 1823 bulls, 1268 young and 555 proven bulls were examined. Three hundred and three bulls appeared to be CVM carriers (16.62%). The highest number of carriers occurred in the sons of a CVM sire, 55.51% and 61.90%, for proven and young bulls, respectively. This very high incidence of CVM carriers forced us to implement a strategy of screening young bulls offered by individual breeders to insemination centers. In effect, the number of CVM carriers dramatically dropped among proven bulls born in 2004 and disappeared in bulls born in 2006.
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The effect of insertion/deletion polymorphisms within the promoter and intron 1 sequences of the PRNP gene on the breeding value of Holstein-Friesian bulls

72%
Czarnik U., Strychalski J., Olenski K., Barcewicz M., Hering D. M., Puckowska P., Pierzchala M., Urbanski P., Pareek C. S.
Animal Science Papers and Reports
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2015
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tom 33
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nr 1
The aim of this study was to verify the hypothesis that insertion/deletion (indel) polymorphisms within the promoter and intron 1 sequences of the prion protein (PRNP) gene can affect the breeding value of Holstein-Friesian bulls. The experimental material included 261 Holstein-Friesian bulls born between 1997 – 2002. It was shown that the polymorphism in the intron 1 (12 bp indel) sequence had a more significant effect on the analyzed traits than a polymorphism in the promotor (23 bp indel) sequence. A deletion allele within intron 1 (12del) significantly increased the bulls’ breeding value for milk yield (p=0.001), protein yield (p=0.042), type and conformation (p=0.018),udder width (p=0.003), dairy character (p=0.004), and decreased days open (p=0.022). A deletion allele (23del) at the polymorphic locus of the promoter significantly decreased the bulls’ breeding value for milk yield (p=0.001), udder width (p=0.029) and dairy character (p=0.033). Analysis of both alleles showed that 23del-12del haplotype increased the bulls’ breeding value for udder traits:udder (p=0.008), fore udder (p=0.043) and udder depth (p=0.041), and decreased for fat kontent (p=0.047) and conformation traits - body depth (p<0.001), chest width (p=0.029) and rear leg set - side view (p=0.029). Also positive effect of 23del-12ins haplotype for days open (p=0.003) and days between calving and first insemination (p=0.045) was observed.
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