Simulation analysis of correlation between the occurrence of the defined alleles of Ovar-DRBl gene in genotype and the health status of the sheep udders. In simulation analysis, the results of the molecular studies (RFLP of DRB1 gene) and the results of evaluation of the health status of udder (SCC in 1 ml of milk) being conducted on Polish Heath Sheep and Polish Lowland Sheep of Żelaźnieńska variety were utilized. Besides it, the data base for simulation analyses was created from the nucleotide sequences of exon 2 of Ovor-DRB 1 gene, and their corresponding amino acid sequences, published in GenBank. The simulation cutting of the collected sequences with restriction enzymes (BstYl, ifauRI, Rsal) was performed and then, the comparison with the real results of restriction analysis and with the results of evaluation of health status of the udders of the examined sheep was carried out. In the female carriers of the allele 141 (fo/YI, BsuRl, Rsal) the statistically significantly higher (P < 0.05) mean somatic cell count in milk was recorded; and in the female carriers of allele 112, the lower mean somatic cell count in milk was found, as compared to the female carriers of the remaining alleles. Due to the fact that the mentioned alleles in the examined ewes may have an antagonistic effect, the comparison of virtually created sequence of allele 141 and the sequence, corresponding to allele 112 was carried out. Twenty six nucleotide changes were found between the mentioned above sequences what corresponded to 8 changes in sequence of amino acids. The recorded correlation between the defined alleles of Ovar-DRB 1 and health status of the sheep udders (SCC) indicates that the developed procedure of simulation analyses may be utilized in breeding practice.
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