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Background: It has been reported that the expression of activating transcription factor 3 (ATF3) is closely associated with both microRNA (miRNA) processing and the progress of many cancers. Our study aimed to explore the interaction between ATF3 and miR-488 in tongue squamous cell carcinoma (TSCC). Methods: Quantitative real-time PCR was performed to detect the levels of ATF3 and miR-488 in TSCC tissues and cell lines. Cell invasion and epithelial–mesenchymal transition (EMT) were assessed to determine the biological functions of miR-488 and ATF3 in TSCC cells. The mRNA and protein levels of ATF3 were measured using quantitative RT-PCR and western blotting. Luciferase assays were performed to validate ATF3 as an miR-488 target in TSCC cells. Results: We found that the level of miR-488 significantly decreased and the expression of ATF3 significantly increased in TSCC tissues and cell lines. A low level of miR-488 was closely associated with increased expression of ATF3 in TSCC tissues. Introducing miR-488 significantly inhibited the invasion and EMT of TSCC cells, and knockdown of miR-488 promoted both processes. The bioinformatics analysis predicted that ATF3 is a potential target gene of miR-488. The luciferase reporter assay showed that miR-488 could directly target ATF3. ATF3 silencing had similar effects to miR-488 overexpression on TSCC cells. Overexpression of ATF3 in TSCC cells partially reversed the inhibitory effects of the miR-488 mimic. Conclusion: miR-488 inhibited cell invasion and EMT of TSCC cells by directly downregulating ATF3 expression.
Competition among trees is a fundamental interaction process within plant community, which is the theoretical basis of thinning. Plant competitive intensity is generally measured using a competition index (CI) that can be classified into two major categories: distance-independent and distance-dependent. The current study used Cunninghamia lanceolata (Lamb.) Hook as the test subject and used Hegyi's CI (distance-dependent), to quantify individual CI and their relationship with tree diameter at breast height (DBH). Five different criteria were used to select potential competitors for the calculation of CI. Seven basic linear and nonlinear mathematical functions were used to test and quantify the relationships between DBH of the target tree and the individual CI. Results showed that individual CI was negatively correlated with target tree DBH: as DBH increased, competition intensity weakened. The adjusted R² with five different criteria of selection competitors simulated by seven functions ranged from 0.30 to 0.82. Considering the root mean square error (RMSE), P-value, and adjusted-R², our results suggested that the best model to simulate the relationship between individual CI and focal tree DBH was power function (CI = 43.98 × DBH⁻¹‧⁰⁸, adjusted R² = 0.81) and with the Voronoi diagram method as the criteria for selecting competitors. These results can demonstrate a clearer understanding of the spatial structure of forests, and can be used to guide the selection of thinning trees in the process of thinning practice.
Steel enterprises, as one type of major industrial pollution source, have been facing great environmental pressure. Integrating environmental considerations into the steelmaking process is now one of the major priorities of the steel industry. A method for evaluating environmental performance is presented in this paper. The proposed method considers the procedure for environmental performance comparison of design alternatives as a multi-criteria decision-making (MCDM) problem. An integrated environmental index for the steel- making process is proposed and qualified, utilizing the Chinese Standard (emission standard of waste pollutants for iron and steel industry). The pair-wise comparison approach of the analytic hierarchy process (AHP) is employed for solving the MCDM problem. A case study is used to illustrate how the assessment method may be applied and to demonstrate its applicability.
Hydroponics experiment was conducted to identify cadmium (Cd) tolerance and phytoextraction potential of moso bamboo (Phyllostachys pubescens) seedlings grown under different levels of Cd. Application of Cd adversely affected the overall growth and ultrastructural characteristics of moso bamboo. At the highest Cd concentration (400 lM), the growth of moso bamboo seedlings was significantly inhibited, and Cd concentrations in leaves, stems and roots reached the maximum of 25.6, 129.8 and 377 mg kg-1, respectively. Scanning electron microscopy (SEM) revealed that the excessive Cd concentrations caused formation of abundant inclusions in the root and stem. The ultrastructural analysis using transmission electron microscopy (TEM) showed that the excessive Cd concentrations caused abnormal-shaped chloroplasts, disappearance of endoplasmic reticulum, and shrinkage of nucleus and loss of thylakoid membranes. It is suggested that use of moso bamboo as experimental material provides a new perspective for remediation of heavy metal-contaminated soil due to its deep root system and larger biomass. However, mechanisms of Cd uptake and accumulation as well as metal interactions within the plant cell need to be investigated further.
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