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Hairy root culture of Picrorhiza kurroa Royle ex Benth.: a promising approach for the production of picrotin and picrotoxinin

100%

Mishra J., Bhandari H., Singh M., Rawat S., Agnihotri M., Mishra S., Purohit S.

Acta Physiologiae Plantarum

2011

tom 33

nr 5

Picrorhiza kurroa Royle ex Benth. is an endangered plant producing various compounds of medicinal importance. Hairy roots of P. kurroa were obtained following cocultivation of shoot tip explants with Agrobacterium rhizogenes strains A 4 and PAT 405. Bacterial strain A 4 appeared to be better than the strain PAT 405 in terms of both growth of respective hairy root cultures and secondary metabolite production. The optimal growth of both the hairy root cultures occurred on halfstrength semisolid medium with 3% sucrose. Picrotin and picrotoxinin from the roots of wild type field grown plants were compared with 8-week-old hairy root cultures induced by the A 4 and PAT 405 strains of A. rhizogenes. Picrotin and picrotoxinin content were evaluated in hairy root cultures as well as roots of field grown plant of P. kurroa. In terms of the production of picrotin and picrotoxinin, the A 4 induced hairy roots appeared to be a better performer than the PAT 405 induced hairy root cultures. The picrotin and picrotoxinin content was highest in 8-week-old A 4 induced hairy roots (8.8 µg/g DW and 47.1 µg/g DW, respectively). Rapid growth of the hairy roots of P. kurroa with in vitro secondary metabolite production potential may offer an attractive alternative to the exploitation of this endangered plant species.

In vitro propagation, genetic and phytochemical assessment of Habenaria edgeworthii: an important Astavarga plant

84%

Giri L., Jugran A., Rawat S., Dhyani P., Andola H., Bhatt I. D., Rawal R. S., Dhar U.

Acta Physiologiae Plantarum

2012

tom 34

nr 3

An efficient in vitro propagation protocol for Habenaria edgeworthii Hook. f. ex. Collett using seedderived callus was established. The maximum seed germination was observed in Murashige and Skoog (MS) medium supplemented with 1.0 µM α-naphthalene acetic acid (NAA). Induction of callus was achieved on full and ½-strength MS medium supplemented with 1.0 µM NAA. The highest number of shoot (11.9 shoots/explant) was achieved in MS medium supplemented with 0.1 µM 6-benzyladenine (BA) and 0.01 µM NAA. Further, elongated shoots when transferred to ½-strength MS rooting medium with different auxin concentrations induced roots (41.6–83.3%) and tubers (0–20.8%); however, a maximum of 87.5% rooting was achieved in a plant growth regulator (PGR)-free MS medium. Rooted shoots (plantlets) when transferred to a mixture of soil:sand:perlite (1:1:1 ratio) resulted in 68% survival. Inter-simple sequence repeats (ISSR) markers confirmed the genetic stability among regenerated plants. The phytochemical analysis of tissue culture-raised tubers showed higher phenolic content than wild tuber. The regeneration protocol developed in this study provides a basis for germplasm conservation and harnessing the total phenol and phenolic compounds of H. edgeworthii. Further, the methods can open avenues for application in other Orchidaceous plants of the Indian Himalayan region.

Identification and comparative analysis of Brassica juncea pathogenesis-related genes in response to hormonal, biotic and abiotic stresses

84%

Ali S., Mir Z. A., Tyagi A., Bhat J. A., Chandrashekar N., Papolu P. K., Rawat S., Grover A.

Acta Physiologiae Plantarum

2017

tom 39

nr 12

Ograniczanie wyników

3 Acta Physiologiae Plantarum

3 Rawat S.

1 Agnihotri M.

1 Ali S.

1 Andola H.

1 Bhandari H.

1 Bhat J. A.

1 Bhatt I. D.

1 Chandrashekar N.

1 Dhar U.

1 Dhyani P.

1 Giri L.

1 Grover A.

1 Jugran A.

1 Mir Z. A.

1 Mishra J.

1 Mishra S.

1 Papolu P. K.

1 Purohit S.

1 Rawal R. S.

1 Singh M.

1 Tyagi A.

1 2017

1 2012

1 2011

Wyniki wyszukiwania

Hairy root culture of Picrorhiza kurroa Royle ex Benth.: a promising approach for the production of picrotin and picrotoxinin

In vitro propagation, genetic and phytochemical assessment of Habenaria edgeworthii: an important Astavarga plant

Identification and comparative analysis of Brassica juncea pathogenesis-related genes in response to hormonal, biotic and abiotic stresses