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1
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Effect of ostarine (enobosarm/GTx024), a selective androgen receptor modulator, on adipocyte metabolism in Wistar rats

100%
Leciejewska N., Pruszynska-Oszmalek E., Bien J., Nogowski L., Kolodziejski P. A.
Journal of Physiology and Pharmacology
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2019
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tom 70
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nr 4
2

Effects of gonadectomy and testosterone replacement on number of dynorphin-immunoreactive (minusIR) cells in the arcuate nucleus of the hypothalamus in obese and diabetic male rats

86%
Ziarniak K., Gawalek M., Pruszynska-Oszmalek E., Kolodziejski P. A., Kaczmarek P., Rodak E., Sliwowska J. H.
Acta Neurobiologiae Experimentalis
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2015
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tom 75
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nr Supl.
BACKGROUND AND AIMS: Dynorphins (Dyn) are involved in the regulation of feeding and kisspeptin together with dynorphin and neurokinin B play a role in reproductive functions. Besides primary metabolic health problems occurring in people with obesity and/or diabetes, there are disruptions of the reproductive system (e.g. hypogonadism, infertility). Moreover, alterations in the hypothalamic dynorphin system in food deprived and diabetic animals were reported. However, there is no study looking at changes in number of Dyn-ir neurons in the arcuate nucleus of the hypothalamus (ARC), where integration of metabolism and reproduction may occur in obese and diabetic rats. There were 2 aims: (1) to assess if obesity induced by high fat diet and/or diabetes induced by injections of streptozotocin (STZ) alters the number of Dyn-ir neurons in the ARC in male rats; (2) to examine if gonadectomy (GDX) and testosterone (T) replacement differentially alters the number of Dyn-ir neurons in the ARC in male rats. METHODS: Rats were fed with high fat diet (HFD) or control (C) diet for 5 weeks. Injections of STZ were performed to induce diabetes type 1 (C/STZ) or diabetes type 2 (HFD/STZ) The following groups were obtained: C, C/STZ, HFD, HFD/STZ. Next, animals were divided into 3 groups: gonadectomy (GDX); gonadectomy and T replacement (GDX+T) and control (Sham). Immunocytochemistry for the Dyn was performed. RESULTS: Dynorphin-ir was found in: paraventricular nucleus of the hypothalamus, supraoptic nucleus, ventromedial nucleus, lateral hypothalamus, ARC and median eminence. Preliminary results indicate that there is a slight increase in number of Dyn-ir cells in C/ STZ and a slight decrease in HFD/STZ group. Currently data from GDX and GDX+T animals is analyzed. CONCLUSIONS: If data is confirmed on the bigger sample (currently analyzed), observed changes may contribute both to metabolic and reproductive deficits observed in these animals. Supported by grant NCN 2011/01/B/NZ4/04992.
3

Effects of ovariectomy and sex hormone replacement on the number of neurokinin B-immunoreactive neurons in the arcuate nucleus of the hypothalamus of obese and diabetic female rats

86%
Ziarniak K., Kolodziejski P. A., Pruszynska-Oszmalek E., Sassak M., Dudek M., Sliwowska J. H.
Acta Neurobiologiae Experimentalis
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2019
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tom 79
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nr Suppl.1
INTRODUCTION: The reproductive capacity of mammals is governed by the hypothalamus-pituitary-gonadal (HPG) axis, with gonadotropin-releasing hormone (GnRH) localized on top of it. Neuronal population expressing kisspeptin, neurokinin B (NKB), and dynorphin (KNDy neurons), present in the arcuate nucleus (ARC) of the hypothalamus, are important for regulating GnRH secretion. Both obesity and type 2 diabetes (DM2) are major risk factors for reproductive alterations (e.g., decreased fertility). Because ARC is a site where cross‑talk between metabolism and reproduction occurs, those states may influence KNDy neurons. However, data on the role of metabolic imbalance, gonadectomy, and sex steroid replacement in the regulation of NKB expression are limited, especially in females. AIM(S): The aim of this study was to assess the effects of metabolic disruption (high-fat diet-induced and DM2), ovariectomy, and sex hormone replacement on the number of NKB-immunoreactive (-ir) neurons in the ARC of female rats. METHOD(S): Female rats received a control (C) or high‑fat diet (HFD) for 13 weeks. Streptozotocin injections were performed to induce DM2 in half of the animals from the HFD group. The following groups were obtained: C, HFD, and DM2. Then, animals were divided into three subgroups: ovariectomy (OVX), ovariectomy and estradiol replacement (OVX+E2), and ovariectomy together with estradiol and progesterone replacement (OVX+E2+P4). Metabolic profile was assessed and immunohistochemistry for NKB was performed. RESULTS: There was an effect of operation (p<0.01). In C and DM2, there was a higher number of NKB-ir cells in OVX+E2+P4 vs. OVX. Additionally, in the DM2 group, a higher number of NKB-ir was seen in OVX+E2 vs. OVX. CONCLUSIONS: HFD does not change the response of NKB‑ir neurons to OVX and hormonal replacement. However, in DM2 females, NKB-ir neurons are more sensitive to the OVX+E2 condition. FINANCIAL SUPPORT: Supported by NCN grant 2015/17/B/NZ4/02021.
4
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Chronic orexin-A (hypocretin-1) treatment of type 2 diabetic rats improves glucose control and beta-cell functions

59%
Kaczmarek P., Skrzypski M., Pruszynska-Oszmalek E., Sassek M., Kolodziejski P. A., Billert M., Szczepankiewicz D., Wojciechowicz T., Maechler P., Nowak K. W., Strowski M. Z.
Journal of Physiology and Pharmacology
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2017
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tom 68
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nr 5
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