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The cytotoxic effect of diphtheria toxin on the actin cytoskeleton

100%

Varol B., Bektas M., Nurten R., Bermek E.

Cellular and Molecular Biology Letters

2012

tom 17

nr 1

Diphtheria toxin (DT) and its N-terminal fragment A (FA) catalyse the transfer of the ADP-ribose moiety of nicotinamide adenine dinucleotide (NAD) into a covalent linkage with eukaryotic elongation factor 2 (eEF2). DT-induced cytotoxicity is versatile, and it includes DNA cleavage and the depolymerisation of actin filaments. The inhibition of the ADP-ribosyltransferase (ADPrT) activity of FA did not affect the deoxyribonuclease activity of FA or its interaction with actin. The toxin entry rate into cells (HUVEC) was determined by measuring the ADP-ribosyltransferase activity. DT uptake was nearly 80% after 30 min. The efficiency was determined as Km = 2.2 nM; Vmax = 0.25 pmol.min−1. The nuclease activity was tested with hyperchromicity experiments, and it was concluded that G-actin has an inhibitory effect on DT nuclease activity. In thepresence of DT and mutant of diphtheria toxin (CRM197), F-actin depolymerisation was determined with gel filtration, WB and fluorescence techniques. In the presence of DT and CRM197, 60–65% F-actin depolymerisation was observed. An in vitro FA-actin interaction and F-actin depolymerisation were reported in our previous paper. The present study thus confirms the depolymerisation of actin cytoskeleton in vivo.

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Effect of breast cancer and breast cancer treatment on the blood serum concentrations of trace elements and selenoproteins

51%

Ogut S., Bahtiyar N., Mordeniz C., Cinemre F. B., Aydemir B., Karacetin D., Degirmencioglu S., Haciosmanoglu E., Kural A., Kiziler A. R., Gunes M. E., Bektas M.

Journal of Elementology

2022

tom 27

nr 2

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Elementology

2 Bektas M.

1 Aydemir B.

1 Bahtiyar N.

1 Bermek E.

1 Cinemre F. B.

1 Degirmencioglu S.

1 Gunes M. E.

1 Haciosmanoglu E.

1 Karacetin D.

1 Kiziler A. R.

1 Kural A.

1 Mordeniz C.

1 Nurten R.

1 Ogut S.

1 Varol B.

1 2022

1 2012

Wyniki wyszukiwania

The cytotoxic effect of diphtheria toxin on the actin cytoskeleton

Effect of breast cancer and breast cancer treatment on the blood serum concentrations of trace elements and selenoproteins