Wyniki wyszukiwania

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Wigor ziarniaków porośniętych w kłosach u odmian pszenżyta ozimego

100%

Mos M., Wojtowicz T.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2001

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nr 220

119-125

Wigor ziarniaków poddanych indukcji porastania w kłosach badano dla odmian pszenżyta ozimego: Bogo, Moreno, Vero w latach 1998–2000. Za pomocą testu c2 stwierdzono wpływ odmiany na występowanie zróżnicowanych uszkodzeń porostowych ziarniaków. Niezależnie od udziału poszczególnych grup ziarniaków z uszkodzeniami porostowymi, wigor i zdolność kiełkowania wykazały istotne współdziałanie odmian z latami. Odmiana Moreno o najwyższym procentowym udziale ziarniaków bez widocznych uszkodzeń porostowych charakteryzowała się największą zmiennością zdolności kiełkowania, która wahała się od 50,5–86,5% oraz wskaźników wigoru. Podobnie, dużą zmienność badanych parametrów stwierdzono dla ziarniaków kontrolnych tej odmiany.

2

Wplyw temperatury na kielkowanie wybranych odmian pszenzyta jarego

100%

Mos M., Wojtowicz T.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2004

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nr 231

191-200

3

The effect of sprouting damage on the vigour of spring triticale seeds

100%

Wojtowicz T., Mos M.

Acta Physiologiae Plantarum

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2004

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tom 26

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nr 3 suppl.

4

Erythropoietin affects GABAergic transmission in hippocampal neurons in vitro

100%

Wojtowicz T., Mozrzymas J. W.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 4

649-655

Erythropoietin is a potent regulator of erythropoiesis. It acts via the specific membrane receptor (EpoR). Erythropoietin is also known to be present in the central nervous system, and its concentration and the expression of EpoR change during development, which raises the possibility that this modulator might be involved in the regulation of neuronal functions in the developing brain. The GABAergic system undergoes profound changes during development and is particularly susceptible to modulation by endogenous factors. Therefore, we decided to investigate the impact of Epo on GABAergic transmission in hippocampal neurons developing in vitro. An analysis of miniature IPSCs (mIPSCs) revealed that a long-term treatment with Epo (48 or 72 h) resulted in a major acceleration of the decaying phase of these currents while the amplitude and current frequency remained unchanged. Interestingly, this effect was restricted to the youngest considered age group (6-8 DIV), indicating that Epomediated modulation of mIPSCs depends on the developmental stage of the neurons. We conclude that Epo may exert a modulatory action on GABAergic transmission in developing neural networks.

5

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Wpływ stopnia dojrzałości wiech na wartość siewną nasion Festuca pratensis

100%

Wojtowicz T., Mos M.

Łąkarstwo w Polsce

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2011

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tom 14

The aim of this study conducted in the years 2006–2008 was to evaluate the effect of harvest date and varietal variation on sowing value of seeds threshed and shattered from panicles with different degree of maturity in four meadow fescue cultivars. Germinability as well as weight of 1000 seeds were strongly affected by varietal factor and interaction of this factor with harvest date in successive years. Seeds threshed from panicles in the third degree of maturity were characterized by high germinability (84%) but the highest value of this index (87%) was estimated for seeds obtained during induced shattering, notwithstanding the lowest weight of 1000 shattered seeds (2.115 g). Inconsiderable variation of germinability of threshed seeds between applied dates of harvest in analyzed three-year period points to the possibility of harvesting at the earlier date to reduce losses in seed yield caused by shattering process.

6

Wykorzystanie analizy wskaźnikowej w ocenie zdolności kredytowej przedsiębiorstwa – szanse i zagrożenia

100%

Wojtowicz T., Wojcicka A.

Zeszyty Naukowe Szkoły Głównej Gospodarstwa Wiejskiego. Ekonomika i Organizacja Gospodarki Żywnościowej

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2009

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nr 78

7

Blattin Crystalyx Vitalyx - więcej niż lizawka. Wpływ lizawki Crystalyx Vitalyx na wskaźniki rozrodu krów oraz wydajność i skład mleka

100%

Kowalski Z. M., Wojtowicz T.

Hoduj z Głową - Bydło

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2011

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nr 1

8

Changes in the vigour of spring triticale seeds with sprouting damage during storage

100%

Wojtowicz T., Mos M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2010

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tom 545

The aim of the studies was to determine changes in the vigour of seeds of three spring triticale (x Triticosecale Wittm.) cultivars with various sprouting damages occurring during storage. It was found that the changes in vigour indices are mostly affected by sprouting damage and storage time, as well as by the interaction of these factors. The greatest significant reduction in the length of leaves and roots and the decrease in their dry weight, ranging from 25% to 90% as compared to the control, were observed in the most sprouted seeds. Seeds stored for eight months were characterized by the lowest electroconductivity values, which ranged from 117 to 157 μS·cm-1·g-1, and by the greatest respiration intensity. After 20 months of storing an increased, by 50%, outflow of electrolytes and decreased, in proportion to the seed damage degree, respiration intensity were observed. The correlation between seed respiration intensity and exudate electroconductivity, determined at that time, proved to be significant (r = -0.616*).

9

Matrix matalloproteases regulate the extent of synaptic plasticity and spike potentiation in hippocampal CA3 association network

100%

Wojtowicz T., Mozrzymas J. W.

Acta Neurobiologiae Experimentalis

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2012

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tom 72

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nr 2

Matrix metalloproteinases (MMPs) activity support spatial and associative learning in vivo however the underlying mechanisms are poorly understood. It has been shown that MMPs inhibition impairs the maintenance of long-term potentiation (LTP) in hippocampal Sch-CA1 synapses and in mfCA3 pathways. However, it is not known whether MMPs activity influences the scaling of postsynaptic neuronal responses (population spikes, PS) typically associated with synaptic LTP. Therefore, we investigated the plasticity of evoked synaptic transmission (input) and resulting PS (output) in CA3 associative network by simultaneous double electrode extracellular field potentials recording. We found that highfrequency-induced (4 × 100 Hz) LTP of EPSPs and PS was significantly impaired in the presence of broad spectrum MMPs inhibitor FN439 while the EPSP-to-Spike (E-S) curves were right-shifted indicating that less neurons were recruited to fire for a given input. Additionally, these effects could be mimicked by application of MMP-3 inhibitor (NNGH). MMPs inhibitors did not affect input–output function of the CA3 neurons or various forms of short term-plasticity. In conclusion, MMPs and particularly MMP-3 may play an important role in the maintenance of E-S potentiation in the CA3 network and thereby may regulate formation of memory traces in this hippocampal region. Supported by IP2010_047870 grant.

10

The effect of sprouting damage on the yield of winter triticale seeds

100%

Mos M., Wojtowicz T.

Acta Agraria et Silvestria. Series Agraria

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2002-2003

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tom 41

During the period of three successive years, in microplot experiments, the effects of sprouting damage of winter triticale seeds on yield, number of spikes, weight of 1,000 seeds and yield of seeds per spike were investigated. The significance of variability sources was determined on the basis of a test carried out according to the mixed model in which years were the random factor and the degrees of sprouting damage and cultivars were the constant factors The results obtained point to a significant decrease in the number of spikes in plants developed from the most sprouted seeds and, in consequence, to a decrease in yield in unfavourable years.

11

Fitosocjologia w łąkarstwie dziś i w przyszłości

100%

Wojtowicz T., Mos B. M.

Łąkarstwo w Polsce

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2009

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tom 12

12

Culture conditions severely alter the proportions between GABA-and glutamate-evoked currents in hippocampal pyramidal neurons

81%

Szczot M., Wojtowicz T., Mozrzymas J.

Acta Neurobiologiae Experimentalis

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2009

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tom 69

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nr 3

Cultured hippocampal cells are commonly used as a convenient model but non-physiological conditions (impaired homeostasis, lack of glial cells etc.) persisting over long period of time raise concerns. Acute brain slices are believed to better maintain the physiological features but are often problematic for technical reasons (e.g. complicated access to neurons, attenuated spread of pharmaceutics). Non-physiological conditions in cell cultures during maturation of GABAergic and glutamatergic systems may alter expression of GABAA and glutamate receptors affecting thus the excitation to inhibition balance. Proportion between GABA and glutamate receptors may be evaluated by determining ratio between amplitudes of current responses to saturating agonist concentrations. We have recorded current responses to ultrafast applications of saturating GABA and glutamate concentrations in hippocampal cell cultures (9–15DIV) and in brain slices from CA1 pyramidal neurons of P19–P23 rats. For cell cultures, GABAergic and glutamergic currents ratio was 2.34 ± 0.45 (n=12) while for the brain slices it was only 0.24 ± 0.02 (n=5). These results provide evidence that non-physiological conditions in cell cultures may cause a dramatic change in expression pattern of GABAA and glutamate receptors. The underlying mechanisms are not known, but we may hypothesize that a homeostatic modulation due accumulation of neurotransmitters in culture medium could be involved. Support: grant no. 070231/Z/03/Z.

13

Impact of matrix metalloprotease inhibitor NNGH on long-term plasticity of NMDARs-mediated EPSPs in mouse CA1 hippocampal region

81%

Wojtowicz T., Brzdak P., Mozrzymas J. W.

Acta Neurobiologiae Experimentalis

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2014

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tom 74

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nr 3

The matrix metalloproteinases (MMPs) play a key role in synaptic plasticity, learning and memory. However, the underlying mechanisms remain poorly understood. Here we studied the role of MMPs inhibitor NNGH (10 µM) in shaping NMDAR-mediated component of synaptic plasticity by recording field potentials in Sch-CA1 hippocampal synapses in mice (P30-P45) brain slices. NMDAR-mediated EPSPs were isolated with AMPA/kainate receptors antagonist DNQX (20 µM) in Mg2+-free solutions. We found that NNGH completely abolished NMDAR-mediated LTP (178.7±12% vs. 75.8±2.9% of baseline at 1 hour post 4×100 Hz stimulation, n=6, P<0.05). The role of specific MMPs in this process is currently investigated. In conclusion, MMPs regulate NMDAR-mediated LTP and most likely postsynaptic calcium influx during long-term neuronal plasticity. Support: Ministry of Science and Higher Education grant NN401541540.

14

Synaptic plasticity at basal and apical dendrites of hippocampal neurons engage unique intracellular cascades and matrix metalloproteases subtypes

81%

Wojtowicz T., Brzdak P., Wojcicka O., Mozrzymas J. W.

Acta Neurobiologiae Experimentalis

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2017

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tom 77

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nr Suppl.1

Cognitive processes such as learning and memory require functional modifications within neural circuits which involve reorganization of existing synaptic connections and modulation of its strength. In addition, neurons can significantly enhance information storage capacity by scaling dendritic and somatic excitability (e.g. EPSP-to-spike potentiation). Proteolysis of extracellular matrix constituents and membrane proteins by matrix metalloproteases (MMP) has recently emerged as a key element in these processes. We identified NMDARs as a target for MMP-3 but not MMP-2/9 immediately following LTP induction. We next applied confocal imaging for nuclear cFos protein in brain slices fixed immediately following electrophysiology studies and Ca2+ imaging for somatodendritic NMDAR-mediated Ca2+ waves. We concluded that long-term hippocampal E-S potentiation limited to stratum radiatum inputs required MMP-3 activity in a narrow time window following enhanced neuronal activity that promotes NMDAR-mediated postsynaptic Ca2+ entry and activation of downstream signaling cascades leading to immediate early genes transcription. Most recently we discovered that in striking contrast to apical dendrites, synaptic plasticity induced at basal dendrites was insensitive to a wide range of broad and subtype specific MMP inhibitors. Thus, stratum radiatum synapses required MMP-3, alpha 5-integrins or protease-activated receptor 1 (PAR-1) and PKC kinase activity for modulation of NMDARs function, unlike stratum oriens synapses. FINANCIAL SUPPORT: National Science Center grant no. SONATA/2014/13/D/NZ4/03045.

15

Analysis of morpho-anatomical stem properties determining its mechanical strength in selected rye cultivars

81%

Wojtowicz T., Grabowska-Joachimiak A., Zielinski A.

International Agrophysics

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2020

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tom 34

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nr 1

16

Role of dopamine D1\D5 receptors in modulating synaptic plasticity at basal and apical dendritic trees of pyramidal neurons in mouse hippocampus

81%

Wojcicka O., Mozrzymas J. W., Wojtowicz T.

Acta Neurobiologiae Experimentalis

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2017

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tom 77

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nr Suppl.1

INTRODUCTION: Deciphering cellular mechanisms of neuronal circuits plasticity remains of key importance in understanding learning and memory. In the hippocampus, CA1 pyramidal neurons receive different amount of excitatory and modulatory dopaminergic inputs to basal and apical dendritic trees. This layer‑specific differential inputs may play a key role in encoding the respective memory traces. However the locus‑specific mechanism of synaptic plasticity remains poorly understood. AIM(S): The aim of the study was to identify to what extent dopamine receptors could regulate AMPARs and NMDARs function in long-term synaptic plasticity within basal and apical dendrites of pyramidal neurons. METHOD(S): We used combination of electrophysiological recordings (fEPSPs) and pharmacological treatment in acute hippocampal slices of adult P45-60 C57BL/6 male mice. RESULTS: High frequency stimulation (HFS, 4×100 Hz every 10 s) of afferent fibers within basal dendrites (stratum oriens, SO) led to significantly larger long‑term potentiation (LTP) of AMPARs-mediated fEPSPs (LTPAMPA) compared to apical dendrites (stratum radiatum, SR). When slices were incubated with dopamine D1/D5 receptors agonist (SKF‑38393 hydrochloride), LTPAMPA was significantly upregulated at SO but not SR synapses. However, in both projections bath applied NMDARs-antagonist (APV) completely abolished LTPAMPA indicating that NMDAR are indispensable in both SR and SO. We next pharmacologically isolated NMDAR‑mediated synaptic field‑potentials and found that D1/D5Rs agonist potentiated these signals in SO but not SR. We next found that priming of D1/D5Rs with its agonist occluded further potentiation of NMDAR function upon HFS. Moreover, application of D1/D5Rs antagonist (SCH23390) prevented gain in NMDAR function at SO, an effect never observed at SR synapses. CONCLUSIONS: Synaptic NMDARs located at basal and apical dendritic trees are differentially modulated by D1/ D5Rs. Dopamine-mediated modulation of NMDARs function could locus‑specific gain in AMPARs function at SO. FINANCIAL SUPPORT: National Science Center SONATA/2014/13/D/NZ4/03045.

17

Impact of metaloproteases on synaptic and E+S plasticity in the hippocampal mosy fiber–CA3 and AC path ways

81%

Morrzymas J. W., Wiera G., Wojtowicz T.

Acta Neurobiologiae Experimentalis

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2013

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tom 73

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nr Suppl.1

Learning and synaptic plasticity is known to involve activity of metalloproteinases (MMPs). Recently, we investigated impact of MMPs on mossy fiber-CA3 (mf-CA3) projection, where LTP is NMDAR-independent and presynaptic, and showed that MMP blockade disrupted LTP maintenance. Using in situ zymography we showed that LTP induction enhances gelatinases activity. Moreover, by means of gelatin zymography, immunohistochemistry and immunofluorescent staining we have shown that this was due to de novo synthesis and activation of MMP-9 (but not MMP-2). Next we found that manipulations in endogenous MMP-9 affected LTP in the mf-CA3 projection. Intriguingly, in slices from both MMP-9 KO and overexperssing rodents, LTP maintenance was nearly abolished. In KO group, LTP could be rescued by administration of active MMP-9. This suggests that LTP maintenance in mf-CA3 pathway requires fine-tuned MMP-9 activity. Neuronal plasticity may involve also changes in excitability and we addressed this issue in associational/commissural synapses formed between CA3 pyramidal neurons. We found that MMPs (MMP-3 and gelatinases) inhibition significantly reduced EPSP-to-spike (E-S) and spike coherence upon LTP indution. Altogether, we show that MMPs play a crucial role in controlling various facets of neuronal plasticity in the mf-CA3 hippocampal projection. Support: NCN grant NN401541540.

18

GABAergic and glutamatergic currents in hippocampal slices and neuronal cultures show profound differences: a clue to a potent homeostatic modulation

81%

Szczot M., Wojtowicz T., Mozrzymas J. W.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 4

501-506

Acute hippocampal slices and primary neuronal cultures are often used with a tacit assumption that basic characteristics of the two models closely resemble each other. The use of the cell cultures, however, may raise controversies because of non-physiological conditions resulting from e.g. glial cells deficit, random neuronal sprouting, lack of specificity in the synaptic connections, impaired homeostasis, etc. Importantly, alteration in neuronal environment, especially when occurring over a prolonged period of time, may give rise to a profound homeostatic modulation. In the present study we have compared the properties of GABAergic and glutamatergic (non-NMDA) currents in pyramidal neurons from hippocampal slices and neuronal cell culture. We show that, most strikingly, amplitude ratio of currents elicited by ultrafast applications of saturating GABA and glutamate was nearly one order of magnitude larger in cultured neurons than that in slices. Miniature IPSCs and EPSCs also showed substantial differences between these two models. In particular, mEPSC amplitudes were larger and more frequent in cultured neurons but their time duration was longer in slices. Miniature IPSCs did not show differences in amplitude when comparing slices and cultures but their time duration was faster and occurrence more frequent in slices. In conclusion, we provide evidence that expression pattern of GABAA and glutamate receptors as well as synaptic current properties in the neuronal cell culture show profound differences with respect to that in the physiological conditions.

19

Correlations and path analysis of the components of Festuca pratensis seed yield

81%

Wojtowicz T., Binek A., Mos M.

Łąkarstwo w Polsce

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2009

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tom 12

Seed yield and yield components were investigated using single meadow fescue plants (Festuca pratensis Huds.) grown in widely spaced rows. Plants of four cultivars (Cykada, Skawa, Skiba and Skra), entered into the EU catalogue, made the representative sample. On the basis of observations and measurements of 20 plants from two harvest dates and three vegetation seasons, the number of days till heading, number of panicles, seed weight per panicle, degree of panicle axis maturity, weight of fallen seeds, the shedding being induced under laboratory conditions, and gross seed yield. For these traits mean values, coefficients of variation, simple correlation coefficients and Wright’s path coefficients were calculated. Over the period of the three-year experiments the greatest variation was found for seed weight per panicle, seed yield per plant and number of panicles, especially in the first harvest year. From among the investigated traits, seed yield per plant was most correlated with the number of panicles per plant (r = 0.480** to r = 0.829**) and seed weight per panicle (r = 0.336* to r = 0.820**). As the number of panicles per plant increased, the weight of fallen seeds increased as well (r = 0.432** to r = 0.620**). The path analysis confirmed the finding that the number of panicles per plant, increasing in successive years, determined the seed yield by 12–23%, 40–46% and 63–74% respectively. The greatest direct effects of the number of panicles and seed weight per panicle on seed yield were observed in the second harvest year.

20

Próba zastosowania komputerowej analizy obrazu w ocenie imbibicji i wigoru ziarna pszenżyta jarego

81%

Mos M., Wiwart M., Wojtowicz T.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2002

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tom 488

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nr 1

Badano proces imbibicji ziarniaków pszenżyta jarego odmiany Gabo przechowywanych uprzednio przez rok, dwa i trzy lata. W ciągu 14 godzin imbibicji dokonywano pomiarów masy oraz powierzchni, obwodu, długości i szerokosci rzutu obrazu ziarniaków. Dodatkowo wykonano jeszcze takie same pomiary w 24 i 26 godzinie pęcznienia. Uzyskane wyniki świadczą o bardzo silnej korelacji między masą pęczniejących ziarniaków a cechami geometrycznymi ich obrazu, zwłaszcza powierzchnią. Wskazuje to na możliwość zastosowania cyfrowej analizy obrazu w badaniach imbibicji ziarna zbóż.

Ograniczanie wyników

Wigor ziarniaków porośniętych w kłosach u odmian pszenżyta ozimego

Wplyw temperatury na kielkowanie wybranych odmian pszenzyta jarego

The effect of sprouting damage on the vigour of spring triticale seeds

Erythropoietin affects GABAergic transmission in hippocampal neurons in vitro

Wpływ stopnia dojrzałości wiech na wartość siewną nasion Festuca pratensis

Wykorzystanie analizy wskaźnikowej w ocenie zdolności kredytowej przedsiębiorstwa – szanse i zagrożenia

Blattin Crystalyx Vitalyx - więcej niż lizawka. Wpływ lizawki Crystalyx Vitalyx na wskaźniki rozrodu krów oraz wydajność i skład mleka

Changes in the vigour of spring triticale seeds with sprouting damage during storage

Matrix matalloproteases regulate the extent of synaptic plasticity and spike potentiation in hippocampal CA3 association network

The effect of sprouting damage on the yield of winter triticale seeds

Fitosocjologia w łąkarstwie dziś i w przyszłości

Culture conditions severely alter the proportions between GABA-and glutamate-evoked currents in hippocampal pyramidal neurons

Impact of matrix metalloprotease inhibitor NNGH on long-term plasticity of NMDARs-mediated EPSPs in mouse CA1 hippocampal region

Synaptic plasticity at basal and apical dendrites of hippocampal neurons engage unique intracellular cascades and matrix metalloproteases subtypes

Analysis of morpho-anatomical stem properties determining its mechanical strength in selected rye cultivars

Role of dopamine D1\D5 receptors in modulating synaptic plasticity at basal and apical dendritic trees of pyramidal neurons in mouse hippocampus

Impact of metaloproteases on synaptic and E+S plasticity in the hippocampal mosy fiber–CA3 and AC path ways

GABAergic and glutamatergic currents in hippocampal slices and neuronal cultures show profound differences: a clue to a potent homeostatic modulation

Correlations and path analysis of the components of Festuca pratensis seed yield

Próba zastosowania komputerowej analizy obrazu w ocenie imbibicji i wigoru ziarna pszenżyta jarego