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Statistical analysis of the associations between phenolic monoterpenes and molecular markers, AFLPs and SAMPLs in the spice plant Oregano

100%

Azizi A., Ardalani H., Honermeier B.

Herba Polonica

2016

tom 62

nr 2

Introduction: Molecular markers are the examples of the contribution of genome technology to medicinal plant breeding through marker-assisted selection (MAS) for pharmaceutical quality. Objective: Forty-two accessions of Origanum vulgare L. originating from Europe were evaluated to detect genomic and chemotypic polymorphisms and to discover possible associations between them. Methods: A total of 477 molecular polymorphisms including 214 AFLP (Amplified Fragment Length Polymorphism) and 263 SAMPL (Selectively Amplified Microsatellite Polymorphic Loci) were used for genotyping. Components in the essential oils were identified and quantified by gas chromatography (GC) and two major compounds (two economically important monoterpenes: carvacrol and thymol) were investigated. Results: Based on results, a relatively high correlation between chemotypic patterns and genetic markers was identified. Associations between traits of interest for essential oils (carvacrol and thymol content) and genetic markers were tested using five statistical methods including three General Linear Model (GLM) and two unified Mixed Linear Model (MLM) approaches. Significant associations were found for 3 AFLP and 20 SAMPL with three key traits including essential oil yield, carvacrol and thymol content. Conclusion: These associations can constitute a useful starting point for marker-assisted selection. Therefore, the results provide the basis for molecular breeding of O. vulgare for pharmaceutical purposes.

Rumen fermentation of lignocellulosic biomass from wheat straw and date leaf inoculated with bacteria isolated from termite gut

76%

Azizi A., Maia M. R. G., Fonseca A. J. M., Sharifi A., Fazaeli H., Cabrita A. R. J.

Journal of Animal and Feed Sciences

2018

tom 27

nr 3

This experiment evaluated the effects of the inoculation of lignocellulosic biomass from wheat straw (LBWS) and date (Phoenix dactylifera L.) leaf (LBDL) with bacteria (Bacillus licheniformis, Ochrobactrum intermedium and Microbacterium paludicola) with lignocellulose-degrading potential isolated from termite gut on the nutritive value of these substrates. Inoculation with B. licheniformis and M. paludicola had a significant effect on chemical composition (organic matter, crude protein and neutral detergent fibre (NDF) contents) of LBWS whereas for LBDL bacterial treatments only tended to affect protein and NDF contents. LBWS inoculated with B. licheniformis and M. paludicola promoted a lower in vitro gas production from soluble fractions, while all bacterial treatments lowered gas production from insoluble but fermentable fractions of LBDL. Bacterial treatments differently affected the nutritive value of LBWS and LBDL – the effects were more marked for LBWS and with the inoculation with B. licheniformis and M. paludicola. None of the bacteria degraded lignin after three weeks of inoculation. More research is needed to evaluate longer bacterial treatments and different bacterial strains.

Ograniczanie wyników

1 Herba Polonica

1 Journal of Animal and Feed Sciences

2 Azizi A.

1 Ardalani H.

1 Cabrita A. R. J.

1 Fazaeli H.

1 Fonseca A. J. M.

1 Honermeier B.

1 Maia M. R. G.

1 Sharifi A.

1 2018

1 2016

Wyniki wyszukiwania

Statistical analysis of the associations between phenolic monoterpenes and molecular markers, AFLPs and SAMPLs in the spice plant Oregano

Rumen fermentation of lignocellulosic biomass from wheat straw and date leaf inoculated with bacteria isolated from termite gut