The microbial community of the air in the Ciechocinek resort was analyzed. Measurement sites were located in the surroundings of inhalators (graduation towers and the “Grzyb” fountain), in the recreation areas (promenade, parks), and urban areas. Microbiological analyses included enumeration of mesophilic bacteria, actinomycetes, mannitol-positive staphylococci, and hemolytic bacteria. The analyses were conducted in accordance with Polish Standard PN89/Z-04111/02. It was concluded that the abundance of bacteria was affected by meteorological conditions (temperature) and the intensity of tourism. The lowest numbers of the investigated types of bacteria were found around the open inhalators.
Prokaryotes from different phylogenetic groups were studied in surface microlayer (SM, up to 100 μm) and subsurface water (SW – 20 cm) in a eutrophic lake over three months (July, August, and October). The abundance of prokaryotes was determined by epifluorescence microscopy after DAPI staining, and phylogenetic diversity was determined by fluorescence in situ hybridization (FISH) with group-specific, fluorescently labeled oligonucleotide probes. In SW bacteria made up most of the entire community of DAPI-stained microorganisms (54-69%) and in SM bacteria made up only 33-44% of DAPI-stained microorganisms. Archaea corresponded to a small fraction of both bacterioneuston and bacterioplankton. The counts of Archaea and bacteria were significantly higher in SW than in SM. Among all proteobacteria included in the research, γ-proteobacteria represented the most abundant fraction: 42-72% in SM and 39-61% in SW. Statistical analysis revealed that the abundance of γ-proteobacteria is positively correlated with temperature and with dissolved oxygen. β-proteobacteria were the least abundant fraction.
Our study attempts to determine the influence of bacterial reduction on sulphate concentrations in groundwaters. A buried valley in a southeastern section of the Gwda River drainage basin was selected for fieldwork near the towns of Wysoka and Bądecz. Deep aquifers in the area are known for their high sulphate concentrations that result from pyrite oxidation by the Thiobacillus denitrificans bacteria. Isotope compositions of SO₄²⁻ ions show ³⁴S enrichment at lower sulphate concentrations, which might indicate bacterial reduction processes. Theoretical curves of SO₄²⁻ isotope composition and ion concentration change, drawn using a Rayleigh formula and the results of the determination of δ¹⁸O (SO₄²⁻) and δ³⁴S (SO₄²⁻) suggest widely varying intensities of the sulphate reduction processes. It can be estimated that bacterial reduction eliminated between 30% and in excess of 50% of the initial concentrations of SO₄²⁻ ions in individual cases.
The research performed pertained to the microflora living in the air of the Ciechocinek health resort. The investigation carried out from May 2005 to April 2006 embraced the marking of the number of the bacteria (mesophilic b., hemolytic b., mannitolo-positive staphylococci, actinomycetes) and fungi on the ground of the entire health resort. The evaluation of the microbiological state of the air was done in compliance with PN86/Z-04111/02 and PN86/ /Z-04111/03. It was established that, on the ground lying within the zone of influence of the open inhalatoriums, the number of investigated microbes had been lower than in the remaining sections of the health resort.
This study was conducted in the Ciechocinek, which is the largest lowland health resort in Poland. Measurement sites were located around open inhalators, in the recreation areas, and in typical urban areas. The survey consisted of enumeration of molds, according to Polish Standard (Ochrona czystości powietrza... PN89/Z-04111/03), and their identification. September was the month with the highest abundance of molds; their concentrations were highest in stations located in the urban section of Ciechocinek, with the lowest around the open inhalators. Molds of the Cladosporium genus were the most common in the tested air, particularly in summer.
Metabolic activity of heterotrophic bacteria isolated from lakes Stęgwica, Jeziorak and Jasne were investigated. A humic substances of commercial preparation by Aldrich-Chemie, as well as natural humic substances, fulvic and humin acids isolated from lake Stegwica were used as a source of energy. The results indicate that 70-80% of bacteria strains are able to utilise the humus matter. The greatest metabolic activity was recorded in bacteria which were supported by natural humic substances isolated from lake Stęgwica; whereas the lowest was noted in the presence of humin acids.
This paper presents the results of resarch on impact of humic substances (HS) on bacterioplancton in eutrophic Lake Jeziorak (north-eastern Poland). In cultures of natural bacteria from the lake water, enriched with 0, 5, 10, 25 and 50 mg dm⁻³ of HS (natural HS – isolated from lake water or commercial HS – sodium salt of humic substance; Sigma-Aldrich), were determined the following parameters: total number of bacteria (TNB), number of heterotrophic bacteria (CFU), bacterial production (BP) (measured with [³H]methyl thymidine method) and general activity of esterases. Natural HS had a more positive impact on bacterial growth, bacterial production and activity of esterases than the commercial preparation HS. The highest TNB and CFU was observed when the water was enriched with 25 mg dm⁻³ of natural HS. All concentrations of the natural HS stimulated bacterial production. The activity of hydrolytic enzymes increased with rising concentrations of natural HS.
The aim of the present study was to determine microbiological air quality in the Ciechocinek health resort and to identify the most common mesophilic bacteria present in the air. Sampling sites were located in the surroundings of inhalators (graduation towers and the “Grzyb” fountain), in recreation areas (promenade, parks), and urban areas. Samples were obtained by the impaction method in a Merck MAS-100 microbial airsampler. Enumeration of mesophilic bacteria was conducted according to the Polish Standard (PN86/Z- 04111/02). Bacterial strains were identified with appropriate API tests (bioMérieux). Due to the specific microclimate and low levels of microbiological air pollution, Ciechocinek can be considered an excellent upper respiratory tract spa. The lowest numbers of the investigated types of bacteria were found around the open inhalators. In the Ciechocinek area bacteria commonly occurring in air prevailed. Gram-positive bacilli (mainly Bacillus genus) were the most common among the isolated bacteria (53.8% of all strains), and Gram-positive cocci (Staphylococcus, Micrococcus) also were abundant (28%). The least abundant were Gram-negative rods (Pseudomonas, Serratia). Potentially pathogenic species (Pseudomonas aeruginosa, Enterococcus faecium, Aeromonas hydrofila) were found sporadically.
The range of solar radiation reaching the air-water inter-phase, medium wave UV radiation, i.e. UVB 290–320 nm and UVA 320–400 nm, is of the highest biological importance due to its harmful effects. Radiation within this range causes DNA damage (lethal effect) or limits the growth of organisms by inhibiting enzyme synthesis, reducing active transport, or by inducing mutations. The studies were carried out in 2007 based on samples water collected from surface microlayer (SM) (up to 150 μm) and subsurface water (SSW) (25 cm) of pelagic zone of eutrophic lake. The representative collection of bacterial strains was isolated from collected samples. The following are measured: the DNA and cellular protein synthesis activity, respiration activity of the bacteria and activity of hydrolytic enzymes in control cultures, subjected to UVB radiation (applied dose 100 mW cm⁻²) and with and without humic substances (HS) (final concentration 100 mg L⁻¹) playing role of compounds potentially protective from UV radiation. UVB irradiation had the strongest inhibiting impact on production of DNA in bacterial cells (12–23% of that in non-irradiated samples). UVB radiation also inhibits the synthesis of cellular protein (27–43% of that in non-irradiated samples) and bacterial respiration activity (44–48%). UVB radiation had by far the lowest impact on the activity of hydrolytic enzymes. HS may function as a protective agent against UV radiation only in DNA synthesis. No significant differences in response to UVB were found between planktonic and neustonic bacteria.
The present study was aimed at determining the relationship between the operation of municipal landfills and the fluctuations of bacterial indicators of the sanitary quality of soil. Soil samples were collected at monthly intervals from May 2010 to April 2011 at ten sampling sites located on the premises and in vicinity of the reclaimed Municipal Landfill Site (MLS) and the new Municipal Solid Waste Management Plant in Toruń (WMP). Bacteriological analyses (the titre of coliforms, the titre of Clostridium perfringens, and the presence of Salmonella) were conducted according to the Microbiological and parasitological guidelines for the assessment of the sanitary quality of soil issued by the Institute of Rural Health in Lublin. The results obtained led to distinguishing three zones of impact of the facilities on soil. Good sanitary quality of soil was determined 100 m east of the edges of the landfill cells.
The present study was aimed at evaluating the number of bacteria and mould fungi in the indoor and outdoor environment of Toruń University Library. The sampling sites were located in the rooms serving the functions typical of libraries (i.e. in the Main Reading Room, Current Periodicals Reading Room, Collections Conservation Laboratory, Old Prints Storeroom, in rooms serving other (non-library) functions (i.e. main hall, cafeteria, and toilet) as well as outside the library building. The analyses reveal that the concentrations of bacterial as well as fungal aerosols estimated with the use of the impaction method ranged between 101-103 CFU·m-3, which corresponds to the concentrations normally observed in areas of this kind. Evaluation of the hygienic condition of the studied areas was based on the criteria for microbiological cleanliness in interiors submitted by the European Commission in 1993. According to this classification, the air was considered to be heavily or moderately contaminated with bacteria, while the air contamination with mould fungi was described as low or moderate. The air in the Old Prints Storeroom was considered the least contaminated with microbial aerosol.
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