This investigation aimed to study the effect on fertility parameters of one or two artificial inseminations (AI) and the administration (carazalol) before insemination of β-adrenoceptor blocker in Awassi sheep. A total of 144 Awassi sheep with their oestrous synchronized were divided into 4 groups. Group A1 had a single artificial insemination applied at 52 hours after (the sponge was removed), group A2 had a single artifical insemination applied while for preinsemination 0.01 mg/kg carazalol were given i.v. Group B1 had artificial insemination appled twice at 52 and 60 hours. B2 group had artificial insemination appled twice at 52 and 60 hours and for preinsemination 0.01 mg/kg carazalol were given i.v. As a results, the ratios of not observed oestrus after 25 days were 55.6%, 61.1%, 63.9% and 69.4% in groups A1, A2, B1 and B2 respectively. The ratios of pregnancy were diagnosed ultrasonographically after the 30th day of artificial insemination as 47.2%, 50.0%, 52.8% and 61.1% in groups A1, A2, B1 and B2 respectively. The ratios of birth by means of artificial insemination were 41.7%, 47.2%, 47.2% and 55% in groups A1, A2, B1 and B2 respectively. The degree of fertility was found to be higher in the group in which the sheep applied artificial insemination twice with 8 hours intervals and administrated β-adrenoceptor blocker preinsemination. However, the difference between groups was not significant (p>0.05).