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For pretreating waste-activated sludge, the combined alkali and microwave pretreatment is a promising hybrid method. To make this pretreatment more economical, moderate conditions were applied for central composite design of the experiments. A quadratic model was established to describe the influence of NaOH dosage and microwave processing time on methane production. The optimal condition was 0.12 g NaOH/g TS treatment for 24 h and 240 W microwave treatment for 10 min. Either single or hybrid pretreatment can significantly accelerate the hydrolysis, but the degree of degradation increases positively as correlated with treatment intensity. Comparing to untreated sludge, the methane yields of microwave-, alkali-, and combined-treated increased to 1.9, 3.4, and 4.6 times, respectively. The SCOD removal rates of the three kinds of treated sludge were 20.6%, 23.0%, and 36.5%, respectively. The single microwave pretreatment efficiently broke the sludge flocs and promoted the release of biodegradable organics as well as nutrients, but there was no advantage in methane production. The existence of NaOH not only eliminated the inhibition caused by microwave, but also improved the degree of degradation.
Marine transportation is the most important transport mode of in the international trade, but the maritime supply chain is facing with many risks. At present, most of the researches on the risk of the maritime supply chain focus on the risk identification and risk management, and barely carry on the quantitative analysis of the logical structure of each influencing factor. This paper uses the interpretative structure model to analysis the maritime supply chain risk system. On the basis of comprehensive literature analysis and expert opinion, this paper puts forward 16 factors of maritime supply chain risk system. Using the interpretative structure model to construct maritime supply chain risk system, and then optimize the model. The model analyzes the structure of the maritime supply chain risk system and its forming process, and provides a scientific basis for the controlling the maritime supply chain risk, and puts forward some corresponding suggestions for the prevention and control the maritime supply chain risk
Port as one of the key hubs of international logistics, which has become the main part and the base of global logistics management. The port enterprises, plays an important role in the global supply chain. However, due to the lack of understanding in port supply chain management, coordination between the port enterprises, the integration of business process is not perfect, the lack of information sharing between various organizations, ports enterprises usually failed to fully play its positive role. Based on this, the paper makes the port enterprises as the research object, and introduces the excellent performance mode into the port enterprises. In order to study the port enterprises how to carry out effective quality management, and formation the coordination and integration of upstream and downstream of enterprises, so as to realize the competitive advantage in port logistics
Background: MicroRNAs play important roles in regulation of the cardiovascular system. The purpose of this study was to investigate microRNA-320 (miR-320) expression in myocardial ischemia-reperfusion (I/R) injury and the roles of miR-320 in cardiomyocyte apoptosis by targeting AKIP1 (A kinase interacting protein 1). Methods: The level of miR-320 was detected using quantitative real-time polymerase chain reaction (qRT-PCR), and cardiomyocyte apoptosis was detected via terminal dUTP nick end-labeling assay. Cardiomyocyte apoptosis and the mitochondrial membrane potential were evaluated via flow cytometry. Bioinformatics tools were used to identify the target gene of miR-320. The expression levels of AKIP1 mRNA and protein were detected via qRT-PCR and Western blot, respectively. Results: Both the level of miR-320 and the rate of cardiomyocyte apoptosis were substantially higher in the I/R group and H9c2 cells subjected to H/R than in the corresponding controls. Overexpression of miR-320 significantly promoted cardiomyocyte apoptosis and increased the loss of the mitochondrial membrane potential, whereas downregulation of miR-320 had an opposite effect. Luciferase reporter assay showed that miR-320 directly targets AKIP1. Moreover, knock down and overexpression of AKIP1 had similar effects on the H9c2 cells subjected to H/R. Conclusions: miR-320 plays an important role in regulating cardiomyocyte apoptosis induced by I/R injury by targeting AKIP1 and inducing the mitochondrial apoptotic pathway.
Port shoreline resources are the basis of port and shipping development, and its assessment method has become one of the hot issues in port research. On the basis of constructing a reasonable index evaluation system, this paper constructs the fuzzy evaluation matrix based on the triangular fuzzy analytic hierarchy process and constructs the fuzzy evaluation matrix by using the fuzzy comprehensive evaluation method, and obtains the maximum membership degree of the port shoreline resources. Compared with the traditional port shoreline Resource evaluation methods, the new one got more advantages in objective and quantitative. Finally, Combined with the Nanjing section of the Yangtze River as a case for verification, the results show that the model can accurately solve the problem of resource evaluation of port shoreline
A common approach to increasing biogas production is enhancing the hydrolysis of wheat straw by removing the lignin-using chemicals. However, partial organic matter will also dissolve into chemical solutions during pretreatment, which leads to the loss of organic matter for the anaerobic digestion process. The enzymatic hydrolysis of wheat straw and loss of total organic carbon increased with NaOH concentration and pretreatment time. The results suggest that when evaluating a pretreatment method, not just the hydrolysis but the loss of organic matter should be taken into consideration.
An in vitro cumulative gas production technique was used to evaluate the nutritional characteristics of different botanical fractions of mature maize stover (variety Kexiang No.11). Whole maize stover was separated into eight fractions: leaf blade, leaf sheath, whole stem, upper stem, lower stem, cob, husk, and tassel. The results showed that there were significant (P<0.05) variations among different botanical fractions in in vitro gas parameters (e.g., the theoretical maximum volume of gas production, the rate constant of gas production, and fermentation lag time). Husk had the highest theoretical maximum gas production and rate constant, while leaf sheath had the longest lag time. The hemicellulose content was positively related (P<0.05) with cumulative gas production at 24, 36 and 48 h of incubation and with the theoretical maximum gas production. The hemicellulose-to-crude-protein ratio was positively related (P<0.05) to the rate constant of gas production. In vitro gas production showed a negative but not significant (P>0.05) relationship with acid detergent fibre (ADF) and neutral detergent soluble (NDS) contents of the morphological fractions.
Five types of kitchen waste (KW) from China – including hop pot (HP), fast food (FF), Hebei cuisine (HC), university canteen (UC), and other mixed KW (Other) – were investigated as feedstock for potential biogas and methane production. The biodegradability of KW was measured using batch anaerobic digestion (AD) tests and feedstock at an inoculum ratio (F/I) of 0.5. Gompertz and Cone models were used to determine the kinetic parameters of KW degradation, biogas, and methane production. Results showed that HP had the highest lag phase time of 5.46 days. Methane production varies with different sources of KW. HP had the highest methane yield of 363.9 mL/g-VSadded as compared to a sample of FF (334.8 mL/g-VSadded), other ( 278.5 mL/g-VSadded), UC (239.2 mL/g-VSadded), and HC (236.0 mL/g-VSadded). The biodegradability of KW ranged from 39.5% to 50.4%. During the AD process a certain amount of floating brown particles (FBP) were formed, which may be the main inhibiting factor of methane production. Analysis of ¹³C NMR and FTIR revealed that the main component of FBP was calcium stearate. The formation mechanism of calcium stearate may contribute to the relatively high lipid content (18.6% to 30.9%) of the KW sample, which subsequently resulted in over-accumulation of long-chain fatty acids (LCFAs) and reaction with Ca²⁺. Using lipid-rich substrates as feedstock may be an efficient approach to adding Ca²⁺ artificially for reducing the inhibition of LCFAs.
The main aim of this study was to screen and identify cadmium-resistant fungus and to characterize its cadmium adsorption. A cadmium-resistant strain (CN35) was isolated from cadmium-polluted paddy soil. Based on morphlogical characteristics, internal transcribed spacers region and β-tubulin gene sequence phylogenesis analysis, the strain was preliminarily identified to be Penicillium sp. This strain was resistant to Cd at 45 mM with Cd adsorption rate up to 83.56%, and also resistant to other heavy metals such as Pb, Zn, and Cu. When Cd²⁺ concentration ranged from 2 to 5 mM, the fungal colony changed from yellow/green to red. The colony morphology was also affected by Cd²⁺ concentrations with protuberances forming on the colony surface at 20 mM. The strain CN35 was found to grow well at pH 4 to 8 at between 24ºC and 37ºC, and the optimal growth conditions were established to be at pH 4 and 30ºC. Fermented liquid of the strain is neither disease-causing nor inhibitory to rice seedling emergence, but rather improves rice seedling and root growth and enhances rice detoxification ability under Cd stress. Thus, the Cd-resistant fungus CN35 has the potential to treat Cd-polluted rice paddies.
The regional carbon budget of the climatic transition zone may be very sensitive to climate change and increasing atmospheric CO2 concentrations. This study simulated the carbon cycles under these changes using process-based ecosystem models. The Integrated Biosphere Simulator (IBIS), a Dynamic Global Vegetation Model (DGVM), was used to evaluate the impacts of climate change and CO2 fertilization on net primary production (NPP), net ecosystem production (NEP), and the vegetation structure of terrestrial ecosystems in Zhejiang province (area 101,800 km2, mainly covered by subtropical evergreen forest and warm-temperate evergreen broadleaf forest) which is located in the subtropical climate area of China. Two general circulation models (HADCM3 and CGCM3) representing four IPCC climate change scenarios (HC3AA, HC3GG, CGCM-sresa2, and CGCM-sresb1) were used as climate inputs for IBIS. Results show that simulated historical biomass and NPP are consistent with field and other modelled data, which makes the analysis of future carbon budget reliable. The results indicate that NPP over the entire Zhejiang province was about 55 Mt C yr-1 during the last half of the 21st century. An NPP increase of about 24 Mt C by the end of the 21st century was estimated with the combined effects of increasing CO2 and climate change. A slight NPP increase of about 5 Mt C was estimated under the climate change alone scenario. Forests in Zhejiang are currently acting as a carbon sink with an average NEP of about 2.5 Mt C yr–1. NEP will increase to about 5 Mt C yr–1 by the end of the 21st century with the increasing atmospheric CO2 concentration and climate change. However, climate change alone will reduce the forest carbon sequestration of Zhejiang’s forests. Future climate warming will substantially change the vegetation cover types; warm-temperate evergreen broadleaf forest will be gradually substituted by subtropical evergreen forest. An increasing CO2 concentration will have little contribution to vegetation changes. Simulated NPP shows geographic patterns consistent with temperature to a certain extent, and precipitation is not the limiting factor for forest NPP in the subtropical climate conditions. There is no close relationship between the spatial pattern of NEP and climate condition.
A two-phase pressurized biofilm (TPPB) system, including a continuously stirred tank reactor (CSTR) and a pressurized biofilm anaerobic reactor (PBAR), was used to produce high calorific biogas without additional upgrading equipment. The influence of organic loading rate (OLR) on biogas and methane production performance was investigated. Three different OLR levels (4, 5, and 6 g-COD/L/d) were applied to the PBAR in sequence. The headspace pressure of PBAR was controlled at 1.0 MPa. Biogas production, gas composition, and process stability parameters were measured. Results found that the highest methane yield of 332.8 mL/g-COD was obtained at OLR of 5 g-COD/L/d. As compared to approximately 90% methane concentration at OLR of 3.1 g-COD/L/d, the methane content in produced biogas was only 76% at OLR of 5.0 g-COD/L/d. The pH value in the pressurized reactor has an important impact on the quality of produced biogas. Further study should focus on the solution strategies of maintaining suitable pH under higher pressure and higher OLR.
Hypoxic-Ischemic Encephalopathy (HIE) is one of the most recognized causes of neurological deficits in children. Cerebral blood flow (CBF) reductions, as seen with HIE, resulting in neuronal injury have not been evaluated in real-time. Photoacoustic Tomography (PAT) is a form of optical imaging which can detect cerebral hemodynamic alterations in a non- invasive, non-ionizing fashion via changes in hemoglobin optical absorption. Further, this technology has the potential to capture cerebral blood volume (CBV) fluctuations and perhaps CBF changes in real-time. We hypothesized that PAT can detect a reduction in cerebral hemoglobin optical absorption, and therefore CBF, in a neonatal model of hypoxia-ischemia. To investigate, P7 rats underwent right carotid artery ligation and exposure to 8% oxygen for 60 minutes while imaged with PAT every 20 minutes. Cerebral hemodynamic alterations, as measured by mean optical absorption (MOA), were calculated as a change from baseline. Global and regional MOA was analyzed using a linear mixed model. Global MOA was reduced within the right hemisphere as compared to the left during hypoxia. Regional differences in MOA were detected between the left and right sides for the middle and posterior cortical regions. Injury was confirmed using immunohistochemistry. We conclude that a reduction in global and regional MOA, and hence CBF, could be identified by PAT in a neonatal rat model of HIE. This is the first study described in the literature utilizing a neonatal rat model of HIE to demonstrate in vivo alterations in cerebral hemodynamics in a non-invasive and near real-time fashion.
The relationship between litter decomposition and forest succession in addition to the influence of climate variables on the rate of litter decomposition in forest ecosystems are poorly understood. In this study, the effects of forest successional stages, climate, and litter quality on litter decomposition rates were investigated in five sites located in China. The selected sites cover 29 degrees of latitude from 18° N to 47° N and spans more than 5,000 km in length along a temperature gradient that transverses across eastern China. This zonal gradient includes five climate zones from temperate to subtropical to tropical zones. Forest types include broad-leaved Korean pine, deciduous broad-leaved, evergreen broad-leaved, monsoon evergreen broad-leaved, and tropical rain forests. The North-South Transect of Eastern China (NSTEC) is one of fifteen international standard transects setup by Global Change and Terrestrial Ecosystems (GCTE). NSTEC is a key component of the International Geosphere-Biosphere Programme (IGBP). The litterbag method was used in this study to determine mass loss and annual decomposition rates of eight tree species (Pinus massoniana Lamb., Cunninghamia lanceolata (Lamb.) Hook., Schima superba Gardn. et Champ., Cinnamomum camphora (L.) Presl., Cyclobalanopsis glauca (Thunb.) Oerst., C. gracilis (Rehd. et Wils.) Cheng et T. Hong, Michelia chapensis Dandy, and Castanopsis eyeri (Champ.) Tutch. Through a timeframe starting in May, 2006, and ending in May, 2008. Litterbags 15 x15 cm and 0.5 x 1.0 mm mesh were filled with 10 g of leaf litter collected from the subtropical forest region and then placed onto the forest floor in triplicate samples for each eight species in all five sites. Three litterbags per species were retrieved from each of the five sites at two month intervals during the two year experimental period. Results suggest that species litter in the climax stage (C. glauca, C. gracilis, and M. chapensis) tended to decompose faster than those in the pioneer stage (P. massoniana and C. lanceolata). Initial phosphorous (P) and nitrogen (N) concentrations of leaf litter were the most critical variables of litter quality in relation to the impact on the rate of litter decomposition. Litter decomposition at different successional stages was found to be directly related to climatic variables such as mean annual precipitation (MAP) and mean annual temperature (MAT). MAP and initial P and N concentrations could therefore be considered good indicators of rates of decomposition.
Habitat quality for many wildlife populations has a spatial component related to the arrangement of habitat elements across large geographic areas. With remote sensing and GIS technology, this paper presents an approach to calculate Habitat Suitability Index (HSI) for Giant Pandas to evaluate the habitat quality. In this paper, a buffer of a given distance (30 km or more) to the Giant Panda distribution area estimated in three national surveys (1974, 1989 and 2002), which is located in Sichuan, Gansu and Shanxi provinces in western China, was used as the study area. In order to study different species group’s habitat quality, the study area is divided into five parts: the Qinling mountain systems, located in the southeast in Shanxi province, the Minshan mountain systems, located in the south in Gansu province and northwest in Sichuan province, the Qionglai mountain systems, the Xiangling mountain systems and the Liangshan mountain systems, located in the west of Sichuan province, conforming to the five big Giant Panda species groups. Three physical environmental factors (elevation, slope and aspect), one ecological factor (vegetation distribution) and several human-influence factors (distances to highways, general roads, inhabitants and rural areas) are selected as the influence factors to calculate HSI. Each factor was reclassified by grid-cell (30 × 30 m per cell) to the suitability index scale from 0 to 1 based on habitat affinities before final calculation. After analyzing the HSI values on the most Giant Panda distribution area, 0.0144 was considered as the threshold habitat quality. Then, HSI was calculated for five mountain systems for three periods conforming to three national surveys (1974, 1989 and 2002). Several benefits to the approach can be highlighted. Firstly, HSI can be used as the standard to evaluate the quality of Giant Panda habitat. Secondly, by using HSI maps from 1974, 1989 and 2002, we can see that the Giant Panda habitat was the largest in 1974, and was then reduced much before 1989. However, by 2002, it had recovered to some extent, which conforms to the habitat data from the three national surveys. Thirdly, the habitat changes in the five mountain systems examined in the study are different. Finally, nature reserves play an important role in the protection of Giant Panda habitat; there are more suitable habitats in nature reserves than non-protected areas.
Cycads are an ancient lineage of plants that originated in the Permian, which are vital to the interpretation of plant ecology. The evidence in the fossil records indicates that the morphological and anatomical features of cycads are remarkably similar to the extant taxa, which has been instrumental in our understanding the connections between the early origins of seed plants and their present-day counterparts. The cycad ecosystem is an important vegetation type throughout geological time. Research on the ecological function of the cycad plays a significant role in the study of evolutionary ecology. In this study, we investigated the biomass, productivity and total carbon storage (total of vegetation, litter, and soil carbon) of cycad (Cycas panzhihuaensis L. Zhou et S.Y. Yang) ecosystems in the National C. panzhihuaensis Reserve of China (latitude 26°37′, longitude 101°35′, at 1635 m altitude) by applying the site-standard tree sampling harvest.Cycads are considered to be rare and endangered species, and are in the list of key protected wild plants in the world. The National C. panzhihuaensis Reserve is in Southwestern China, which area approximately 1358 ha, growing approximately 20 000 C. panzhihuaensis individuals. 20 sample plots, each 5 × 5 m were established in the spring of 2006. The mean height of cycads within the stand was 0.44 m and the mean basal diameter was 23.2 cm. The biomass and productivity data for other communities was compiled from references published over the past 20 years throughout China. The biomass and productivity of cycad ecosystems (8.102 ± 6.880 t C ha⁻¹ and 1.183 ± 0.975 t C ha⁻¹ yr⁻¹, respectively) are smaller than tree fern (Alsophila spinulosa (Wall. ex Hook.) R. M. Tryon) or gymnosperm (Pinaceae, Cupressaceae or Taxodiaceae for representative) ecosystems. The community biomass of Pinaceae-, Cupressaceae- or Taxodiaceae-dominated ecosystems are 6.8, 5.4, and 5.3 times larger than the cycad ecosystem, respectively. The productivity of each is 2.3, 2.8 and 3.8 times larger than the cycad ecosystem. Cycad is an ancient dioecious plant. However, the results show that the differences between the biomass of male and female cycads, as well as the productivity, are not significant.
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