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In this study, different types of cereal grains (maize, wheat, triticale), and their respective distillers dried grains with solubles (DDGS: DDGSM, DDGSW, DDGST) were used. Their effects on parameters expressing nutritional value for ruminants were assessed. The chemical composition of the DDGS was significantly differed (P<0.01), similarly as that of the cereal grains. The DDGS from maize was the highest in fat (103.5 g. kg-1 DM) but the lowest in crude protein (CP) (291.9 g. kg-1 DM) and ADF (159.9 g. kg-1 DM). All DDGS types had similar energy values (ME approx. 12.5 MJ. kg-1 DM, NEL approx. 7.5 MJ. kg-1 DM and NEV approx. 7.6 MJ. kg-1 DM). The DDGS were three times higher in PDIN values than the original grains (P<0.01). DDGSM had lower in situ effective CP degradability (46.5%) than DDGSW (58.5%) and DDGST (76.8%), but the highest and most stable intestinal digestibility (ID) of rumen undegraded CP (94.3%). The most variable intestinal digestibility was found in DDGSW samples (79.6%-92.2%). The acid detergent insoluble CP (ADICP) content (% from total CP) was significantly higher in wheat and triticale DDGS than in the original grains. There were significant plant effects on fat, ME, NEV, ADICP (P<0.01), ADF, and NADIP (P<0.05). We can conclude that all types of DDGS are a good source of CP and energy for ruminants.
The effect of Enterococcus faecium CCM7420 (EF) – enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.
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