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Wplyw inhibitorow ukladu renina-angiotensyna na obraz morfologiczny trzustki w eksperymentalnym przewleklym zapaleniu tego narzadu

100%

Madro A., Czechowska G., Korolczuk A., Celinski K., Slomka M., Prozorow-Krol B., Korobowicz E.

Medycyna Weterynaryjna

2008

tom 64

nr 01

97-100

Chronic pancreatitis (CP) is a progressive disease, in which the exocrine function of the gland is gradually lost and fibrosis develops due to repeated episodes of acute pancreatitis. The detection of the renin-angiotensin system (RAS) brings us closer to understanding the pathogenesis of CP. It has been observed that the use of RAS inhibitors reduces hepatic fibrosis. The aim of the study was to examine the effects of RAS inhibitors on fibrotic processes in the course of experimental chronic pancreatitis induced by dibutyltin dichloride (DD). Chronic pancreatitis was induced by administering dibutyltin dichloride to the femoral vein and, simultaneously, captopril, losartan and enalapril which were administered intraperitoneally. The rats were decapitated after 60 days and pancreas tissue was collected for histopathology examination. No pathological changes were observed in the control group. Rats treated by dibutyltin dichloride displayed features of focal inflammatory infiltration, ductal lumen dilatation, fibrosis in the periductal spaces and slight interstitial fibrosis. Animals treated by RAS inhibitor displayed less severe inflammatory changes and fibrosis - particularly those rats treated by enalapril. The findings suggest that enalapril most effectively inhibits inflammatory changes and fibrosis.

RAS inhibitors decrease apoptosis of acinar cells and increase elimination of pancreatic stellate cells after in the course of experimental chronic pancreatitis induced by dibutyltin dichloride

100%

Madro A., Korolczuk A., Czechowska G., Celinski K., Slomka M., Prozorow-Krol B., Korobowicz E.

Journal of Physiology and Pharmacology. Supplement

2008

tom 59

nr 2

Chronic pancreatitis (CP) is a progressive disease, in which the exocrine function of the gland is gradually lost and fibrosis develops due to repeated episodes of acute pancreatitis. The aim of the study was to investigate the effects of RAS inhibitors on the apoptosis of acinar cells and pancreatic stellate cells (PSCs) elimination in experimental CP induced by dibutyltin dichloride (DBTC). CP was induced by administration of DBTC to the femoral vein. Simultaneously captopril, losartan, enalapril and lisinopril were administered intraperitoneally. The rats were decapitated after 60 days and tissue of pancreas was collected. In rats treated by DBTC the features of inflammatory infiltration, ductal lumen dilatation, fibrosis were found. Strong reactivity with capsase2L and clusterin-ß antibodies was observed in areas of fibrosis. In animals treated with RAS inhibitors inflammatory changes and fibrosis were less severe. In groups of rats treated with DBTC and RAS inhibitors immunoreactivity of capsase2L and clusterin-ß was weak. Positive immunostaining against smooth muscle actine and desmin was observed in the elongated cells (PSC-s). This reaction was weak in groups of rat treated with DBTC and RAS inhibitors. Treatment of CP rats with RAS inhibitors alleviate apoptosis of pancreatic acinar cells and induces PSCs elimination.

Effects of adenosine receptor agonists and antagonists on cytochrome P450 extinction in the liver of rats in the course of acute pancreatitis

84%

Celinski K., Madro A., Czechowska G., Slomka M., Prozorow-Krol B., Biskup W., Malm A., Juda M., Wielosz M.

Medycyna Weterynaryjna

2007

tom 63

nr 05

528-531

In the course of acute pancreatitis the liver is an organ that is especially exposed to damage. The presence of adenosine receptors was observed in the whole digestive system. The aim of the experiment was to define the correlation between the extinction of cytochrome P450 in the liver of rats and adenosine receptor agonists and antagonists in the course of necrotizing acute pancreatitis. The experiments were carried out on Wistar male rats weighing 250 g. Acute pancreatitis was induced injecting 5% sodium taurocholate to the biliary-pancreatic duct. Prior to the induction of acute pancreatitis the animals were injected intraperitoneally with selective agonists and antagonists: CGS 21680 (selective A2 agonist), 3 mg/kg, ZM 241385 (selective A2a antagonist), 3 mg/kg, DPCPX (A1 antagonist), 1 mg/kg, 1.3-Dipropyl-8-phenylxantine (selective A1 antagonist), 3 mg/kg, IB-MECA (A3 agonist), 0.75 mg/kg. The determinations were performed in hepatic microsomes obtained according to Guegenrichs method Cytochrome P450 extinction was determined by Matsubars technique. The results obtained reveal statistically significantly decreased cytochrome P450 extinction after sodium taurocholate administration. Decreased levels of extinction were also observed after combined administration of sodium taurocholate + Phenylxantine and sodium taurocholate + ZM. The level of IB-MECA remained unchanged in comparison to the controls. However DPCPX and CGS administration increased the extinction of cytochrome P450. The diverse influence of adenosine receptor agonists and antagonists used in the experiment on cytochrom P450 extinction seems to modify the course of the inflammatory process after using 5% sodium taurocholate.

Ograniczanie wyników

2 Medycyna Weterynaryjna

1 Journal of Physiology and Pharmacology. Supplement

3 Celinski K.

3 Czechowska G.

3 Madro A.

3 Prozorow-Krol B.

3 Slomka M.

2 Korobowicz E.

2 Korolczuk A.

1 Biskup W.

1 Juda M.

1 Malm A.

1 Wielosz M.

2 2008

1 2007

Wyniki wyszukiwania

Wplyw inhibitorow ukladu renina-angiotensyna na obraz morfologiczny trzustki w eksperymentalnym przewleklym zapaleniu tego narzadu

RAS inhibitors decrease apoptosis of acinar cells and increase elimination of pancreatic stellate cells after in the course of experimental chronic pancreatitis induced by dibutyltin dichloride

Effects of adenosine receptor agonists and antagonists on cytochrome P450 extinction in the liver of rats in the course of acute pancreatitis