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1

Mykoplazmowe zapalenia gruczołu mlekowego u krów

100%
Szacawa E., Bednarek D.
Weterynaria w Terenie
|
2018
|
tom 12
|
nr 3
2

Ważne czynniki zakaźne układu oddechowego bydła

100%
Szacawa E., Bednarek D.
Weterynaria w Terenie
|
2019
|
tom 13
|
nr 4
3

Aktualna sytuacja epizootyczna zakażeń Mycoplasma bovis u bydła w kraju oraz badania nad ich profilaktyką

81%
Dudek K., Bednarek D., Szacawa E.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2015
|
tom 10
|
nr 2 Monografia
4

Antybiotykooporność i mechanizmy jej powstawania w przypadku zakażeń Mycoplasma bovis u bydła

81%
Szacawa E., Horecka A., Bednarek D., Niemczuk K.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 07
Mycoplasma bovis is the smallest known bacterium that does not have a cell wall. It is therefore resistant to some antibiotics that inhibit the synthesis of the cell structure. Little is known about the mechanisms of antibiotic resistance in M. bovis, since it has no plasmids and there are insufficient data about the role of the biofilm formation by these bacteria. Previous studies have shown that the development of antibiotic resistance is due to gene mutations. Antibiotics generally considered as effective against M. bovis infection are macrolides, fluoroquinolones, tetracyclines, lincosamides, aminoglycosides, and chloramphenicol. Several recent studies, however, indicate that the efficacy of tetracyclines, macrolides, and lincosamides has diminished. Increased resistance to erythromycin, spectinomycin, and tilmicosin, antibiotics commonly used in the treatment of M. bovis infections, has also been reported. Among field strains of M. bovis no resistance or rare resistance has only been observed for enrofloxacin, florfenicol, tylosin, and tulathromycin. Considering the rapidly growing antibiotic resistance of the isolated strains of M. bovis, it is necessary to search for alternative compounds that could effectively inhibit these bacteria.
5

Screening of the Cervidae family in Poland for Mycoplasma species

81%
Dudek K., Bednarek D., Szacawa E., Ayling R. D.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 4
6

Immunomodulatory effect of Mycoplasma bovis in experimentally infected calves

81%
Dudek K., Bednarek D., Ayling R. D., Szacawa E.
Bulletin of the Veterinary Institute in Puławy
|
2013
|
tom 57
|
nr 4
The effect of three different field isolates of Mycoplasma bovis on selected immunological parameters in experimentally infected calves was studied. Calves were kept separately in 4 experimental groups, and animals of 3 groups were infected intratracheally with one of the three selected isolates of M. bovis. The control group was inoculated intra-tracheally with sterile physiological saline. Nasal swabs and blood samples were collected just before the calf inoculation, then daily for seven days, and then weekly for another three weeks. The presence of M bovis antigen, M. bovis antibodies, total protein, gamma globulins, IgA, IgM, IgG, acute phase proteins (haptoglobulin and serum amyloid A), as well as interferon-γ and interleukin-4 concentrations were determined. M. bovis was detected intermittently during the study in the infected groups from day 1, whilst the control group remained free of the pathogen. M. bovis antibodies were detected in some of infected animals in the second, third, and fourth week arter infection. The stimulation and/or immunological suppression varied between the M bovis isolates used for the inoculation. All M. bovis isolates induced a rise of APP and gamma globulin concentrations in infected calves. However, in this study the mucosal immune response appeared to be down-regulated, which was expressed with a general lack of IgA stimulation.
7

Evaluation of immune response in seropositive cattle for Mycoplasma bovis

81%
Dudek K., Bednarek D., Szacawa E.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
The aim of the study was to evaluate the changes of selected immunological parameters in seropositive cattle infected with Mycoplasma bovis. The study was performed on two age groups of cattle, i.e. young (n=139) and adult (n=21) animals. Within the each age group, two subgroups composed of seropositive and seronegative animals for M. bovis infection were formed. The serum samples were collected and the following immunological parameters were determined: Mycoplasma bovis antibodies, total protein, γ-globulins, and selected acute phase proteins - haptoglobin and serum amyloid A. In M. bovis seropositive cattle, the distinct increase in all examined parameters in both age groups was observed as compared with the controls. The results indicated the significant stimulation of immune response in the affected cattle and the performed assays confirmed the real possibility for their use in the screening diagnosis of the bacterial infections.
8

Immunoprofilaktyka swoista zakażeń M. bovis u bydła - wstępne badania nad opracowaniem doświadczalnej szczepionki

81%
Dudek K., Szacawa E., Bednarek D.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2014
|
tom 09
|
nr 2 Monografia
9

Stimulating effect of Mycoplasma bovis infection on proinflammatory response in infected cattle

81%
Dudek K., Bednarek D., Szacawa E.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
The aim of the study was to evaluate the alternations in the concentration of proinflammatory arachidonic acid metabolites in cattle infected with Mycoplasma bovis. The study was performed on two groups of cattle, differing in aged, i.e. calves (n=56) and adult animals (n=19). Additionally, two subgroups were selected among them: seropositive and seronegative for Mycoplasma bovis infection. The presence of serum specific antibodies against Mycoplasma bovis and the concentration of selected inflammatory parameters, such as prostaglandins (PGE₂, PGF₂α), leukotrien B₄, and tromboxan B₂ were determined. An increase in the concentration of the greater part of arachidonic acid cascade metabolites in cattle seropositive for Mycoplasma bovis, in both age groups was observed in comparison with the seronegative animals, except leukotrien B₄ in adult animals. The obtained results indicate the stimulating effect of Mycoplasma infection on proinflammatory response in infected cattle.
10

Biotropina - zastosowanie w immunopotencjalizacji odporności u cieląt

81%
Bednarek D., Dudek K., Szacawa E.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2014
|
tom 09
|
nr 2 Monografia
11

Mycoplasma mycoides subsp. Mycoides small colony variant and Mycoplasma agalactiae antibodies in ruminants in Poland

81%
Dudek K., Bednarek D., Szacawa E.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 4
The aim of the study was to determine Mycoplasma mycoides subsp. mycoides small colony variant (MmmSC) and Mycoplasma agalactiae antibodies in ruminants from different provinces/regions of Poland. Eight hundred and ten bovine serum samples were examined for MmmSC antibodies by the use of competitive ELISA (c-ELISA) and complement fixation test (CFT). ELISA was also used for M agalactiae antibody detection in 951 serum samples of sheep and goats. The first screening serological examination of MmmSC antibodies using c-ELISA revealed two (0.25%) positive and 135 (16.92%) doubtful results. The second examination revealed only 52 doubtful results, whereas the rest samples were negative. To compare, the final confirmatory examination by CFT gave 100% of seronegative results. The examination performed in small ruminants demonstrated only one doubtful result, which was finally defined as negative following the second ELISA, whereas the remaining samples were negative. To conclude, the present serological study showed the lack of infections in Polish domestic ruminant caused by two mycoplasmas.
12

Zakażenia mykoplazmowe istotny problem w utrzymaniu prawidłowego poziomu dobrostanu bydła

81%
Dudek K., Szacawa E., Bednarek D.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2017
|
tom 12
|
nr 2 Monografia
13
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Screening for Mollicutes microorganisms in perinatal calf mortality cases in Polish dairy herds

71%
Szacawa E., Jawor P., Dudek K., Bednarek D., Stefaniak T.
Polish Journal of Veterinary Sciences
|
2018
|
tom 21
|
nr 3
14

Mycoplasma bovis infections and co-infections with other Mycoplasma spp. with different clinical manifestations in affected cattle herds in eastern region of Poland

61%
Szacawa E., Niemczuk K., Dudek K., Bednarek D., Rosales R., Ayling R.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 3
The aim of the study was to evaluate the presence of Mycoplasma bovis infection and co-infections with other Mycoplasma spp. infections in cattle. The tested population was one in the eastern region of Poland containing 66 dairy cows and 23 calves showing different clinical signs and suffering from pneumonia, mastitis, and arthritis. The incidence of M bovis in co-infections with other Mycoplasma spp. was examined using serological traditional mycoplasma culture methods, and the molecular methods - PCR and polymerase chain reaction/denaturing gradient gel electrophoresis (PCR/DGGE). The PCR/DGGE method for detecting Mycoplasma spp. in cattle was used for the first time in Poland. The seroprevalence of M. bovis in the affected cattle herds in the eastern region of Poland was 47.8% in calves and 19.7% in dairy cows. The direct detection and identification of M. bovis from nasopharyngeal swabs by PCR revealed that 56.5% of calves were positive, but all of the dairy cows were negative. The PCR/DGGE identified eight (34.8%) instances of M. arginini and eight (26.1%) instances of M. bovirhinis co-infecting with M. bovis in ten calves. The seroprevalence of M. bovis in the tested population was 33.7%. Any future attempts to control mycoplasma infections require an insight into the current epidemiological situation of M. bovis infection and its relationship to other mycoplasmas in causing clinical disease in cattle. Using these diagnostic methods we have demonstrated that mycoplasmal infections are often caused by multiple species of Mycoplasma and not just the primary M. bovis pathogen.
15
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A serological and molecular study on the occurrence of mycoplasmas in European bison (Bison bonasus) from two areas of Eastern Poland

61%
Dudek K., Bednarek D., Szacawa E., Ayling R. D., Krzysiak M. K., Marczuk J.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
|
nr 4
European bison (Bison bonasus) from two different areas of Eastern Poland showing gross pathology possibly associated with mycoplasma infections were tested for ruminant Mycoplasma species using serological and molecular methods. Fifty-five samples, blood or tissue were collected from 28 animals during 2013-2014. Six sera were positive for Mycoplasma bovis. The ELISA and complement fixation test for Mycoplasma mycoides subsp. mycoides gave a few weak reactions, but were negative by immunoblotting and molecular methods.
16

Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

61%
Szacawa E., Szymanska-Czerwinska M., Niemczuk K., Dudek K., Wozniakowski G., Bednarek D.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 4
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