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1

Przebudowa drzewostanow w Nadlesnictwie Jamy

100%

Tarnawski A.

Przegląd Leśniczy

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2000

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tom 10

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nr 01

12-14

2

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Immunoblot analysis of immune response to cell surface antigens of different Helicobacter pylori strains

81%

Karczewska E., Wyle F., Tarnawski A.

Journal of Physiology and Pharmacology

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1996

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tom 47

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nr 1

3

Helicobacter pylori toxin inhibits growth and proliferation of cultured gastric cells-Kato III

81%

Chang K., Fujiwara Y., Wyle F., Tarnawski A.

Journal of Physiology and Pharmacology

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1993

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tom 44

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nr 1

4

Direct protective action of epidermal growth factor on isolated gastric mucosal surface epithelial cells

81%

Ishikawa T., Fukuda T., Sugiyama M., Tarnawski A.

Journal of Physiology and Pharmacology

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1992

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tom 43

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nr 4

Epidermal growth factor (EGF) protects gastric mucosa against acute injury produced by a variety of damaging agents, but the mechanism of its protective action is not clear. Since the surface epithelial cells (SEC) are important component of gastric mucosal defence, we studied whether EGF may directly protect isolated gastric SEC against ethanol injury in vitro, in condition independent of systemic factors and whether endogenous prostaglandins may play a role in EGF’s protective action. The isolated SEC from rat gastric mucosa were preincubated in medium only, or medium containing 0.0001-10.0 µg/ml of h-rEGF for 15 minutes, and incubated with 8% ethanol for 1 hour. In another study the above experiment was repeated but cells were pretreated with 10⁻⁴ or 10⁻⁵ M indomethacin before EGF treatment. The cell viability was assessed by fast green exclusion test. Incubation of SEC with 8% ethanol significantly reduced SEC cell viability to 50 ₋⁺ 2%: EGF 0.1 or 1.0/µg/ml significantly reduced ethanol induced damage (cell viability 59 ₋⁺ 3 and 62₋⁺ 3% respectively). Pretreatment with 10⁻⁴ M indomethacin (the dose which does not affect SEC viability but inhibit PGE₂ and PGI₂ generation), significantly reduced protective action of EGF against 8% ethanol injury. EGF 1.0 and 10.0 µg/ml alone without ethanol increased PGE₂ and 6 keto PGF₁α generation by SEC. These studies demonstrated: 1) EGF is able to protect gastric surface epithelial cells directly without mediation by systemic factors. 2) EGF induced protection of SEC may in part be mediated by prostaglandins.

5

Expression of serum response factor in normal rat gastric mucosa

81%

Chai J., Baatar D., Moon W., Tarnawski A.

Journal of Physiology and Pharmacology

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2002

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tom 53

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nr 2

Serum response factor (SRF) is a transcription factor that is involved in cell proliferation, muscle and neuron development and maintenance. Its expression in gastric mucosa remains unknown. In this study we demonstrated that SRF is expressed in normal rat gastric tissue as two isoforms and localized mainly to smooth muscle cells of muscularis mucosae and its extensions into the lamina propria, to muscularis propria, and musculature of the vascular system. To a lesser extent, SRF is also expressed in the gastric epithelium of the mucosal neck area (proliferative zone) and in the endothelial cells of microvessels. These data suggest that the main role of SRF in normal gastric tissue is to maintain muscular support and contraction and possibly epithelial regeneration.

6

Effect of secretin on antipyrine elimination during extracorporeal perfusion of the isolated guinea pig liver

71%

Mach T., Goszcz A., Bogdal J., Barylak H., Tarnawski A.

Acta Physiologica Polonica

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1978

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tom 29

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nr 3

7

Histochemistry and ultrastructure of gastric submucosal vasculature in portal hypertensive rats

71%

Sarfeh L. J., Tarnawski A., Stachura J., Hajduczek A., Bui H. X.

Folia Histochemica et Cytobiologica

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1988

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tom 26

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nr 3

8

Inhibitory effects of indomethacin on growth and proliferation of gastric carcinoma cells Kato III

71%

Fujiwara Y., Tarnawski A., Fujiwara K., Arakawa T., Kobayashi K.

Journal of Physiology and Pharmacology

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1993

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tom 44

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nr 2

9

Epidermal growth factor [EGF] expression in human salivary glands. An immunohistochemical study

71%

Dubiel B., Mytar B., Tarnawski A., Zembala M., Stachura J.

Journal of Physiology and Pharmacology

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1992

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tom 43

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nr 1

Epidermal growth factor (EGF) is a biologically active peptide involved in differentiation, growth, regeneration and repair of human and animal tissues. Quantitative biochemical studies showed in man the highest concentration of EGF in the parotid gland. The aim of the present study was to define EGF immunolocalization in the individual segments of the human major salivary glands (salivon). The material consisted of sections obtained from the surgically removed salivary glands: parotid, submaxillary and sublingual. Immunohistochemical studies were performed by PAP method using monoclonal antibody against human epidermal growth factor. EGF expression was found almost exclusively in the efferent pathways of the salivary glands, mostly in the intercalated ducts and Pflüger salivary tubules. These segments of the salivon are most developed in the parotid gland in which the staining was stronger than in other salivary glands.

10

Comparison of prostaglandin and cimetidine in protection of isolated gastric glands against indomethacin injury

62%

Brzozowski T., Tarnawski A., Hollander D., Sekhon S., Krause W. J., Gergely H.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 5

75-88

Prostaglandins can protect the in vivo gastric mucosa against necrosis produced by a variety noxious agents. Cimetidine has also been shown to have protective properties in humans and in some models of experimental injury. Whether prostaglandins or cimetidine may protect gastric mucosal cells directly in the absence of systemic factors remains controversial. In the present study, the potential protective actions of prostaglandin and cimetidine against indomethacin injury were assessed in isolated rat gastric glands. Gastric glands were pre-incubated in oxygenated medium with either placebo, 16,16 dimethyl prostaglandin E2 (dm PGE2) or cimetidine and incubated at 37°C in medium containing 0.5 mg/ml of indomethacin for 2, 4 and 6 hrs. Cell injury and protection was assessed by the Fast Green exclusion test (viability test), leakage of lactate dehydrogenase (LDH) into the medium, and by scanning and transmission electron microscopy. In addition, the generation of PGE2 by the gland cells was determined using RIA assay. Indomethacin by itself significantly reduced the viability of gastric glands, increased LDH release into the medium and produced prominent ultrastructural damage. In contrast to cimetidine, co-incubation of gastric glands with dm PGE2 added to indomethacin, significantly reduced indomethacin-induced injury, increased the number of viable cells, reduced LDH leakage and diminished the extent of ultrastructural damage. The dose of indomethacin (5 µg/ml) which significantly inhibited the generation of PGE2 (up to 90% inhibition) had no effect on cell viability nor LDH release. We conclude that 1) exogenous PGE2 exerts a potent protective activity in vitro which is independent on neural, vascular and hormonal factors; 2) inhibition of endogenous PGs may not the primary mechanism in the deleterious action of indomethacin against damage to gastric glandular cells and 3) indomethacin can exert a direct cytotoxic effect on the mucosal cells in gastric glands.

11

Isolation of morphologically and functionally intact gastric mucosal microvessels rapid communication

61%

Tarnawski A., Arakawa T., Sekhon S., Ichikawa Y., Szabo I., Sarfeh I. J.

Journal of Physiology and Pharmacology

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2000

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tom 51

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nr 1

Ograniczanie wyników

Przebudowa drzewostanow w Nadlesnictwie Jamy

Immunoblot analysis of immune response to cell surface antigens of different Helicobacter pylori strains

Helicobacter pylori toxin inhibits growth and proliferation of cultured gastric cells-Kato III

Direct protective action of epidermal growth factor on isolated gastric mucosal surface epithelial cells

Expression of serum response factor in normal rat gastric mucosa

Effect of secretin on antipyrine elimination during extracorporeal perfusion of the isolated guinea pig liver

Histochemistry and ultrastructure of gastric submucosal vasculature in portal hypertensive rats

Inhibitory effects of indomethacin on growth and proliferation of gastric carcinoma cells Kato III

Epidermal growth factor [EGF] expression in human salivary glands. An immunohistochemical study

Comparison of prostaglandin and cimetidine in protection of isolated gastric glands against indomethacin injury

Isolation of morphologically and functionally intact gastric mucosal microvessels rapid communication